Fluorine-18 labelled Ruppert-Prakash reagent ([ 18 F]Me 3 SiCF 3) for the synthesis of 18 F-trifluoromethylated compounds

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This text describes the primary synthesis and utility of fluorine-18 labelled Ruppert-Prakash reagent [18F]Me3SiCF3. [18F]Me3SiCF3 was synthesized from [18F]fluoroform with radiochemical yields of 85-95% and radiochemical purities of >95% inside 20 minutes. 18F-trifluoromethylated compounds had been efficiently ready by response of [18F]Me3SiCF3 with benzaldehydes, acetophenones and benzophenones.
Intact part II steroid metabolites have poor product ion mass spectra beneath collision induced dissociation (CID) situations. Due to this fact, we current herein the liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/(MS)) habits of intact part II metabolites of oxosteroids after derivatization.
Based mostly on the truth that Girard’s reagent T (GRT), as derivatization reagent, was each handy and environment friendly by way of the enhancement within the ionization effectivity and the manufacturing of diagnostic product ions associated to the steroid moiety, the latter was ideally chosen between methoxamine and hydroxylamine upon the mannequin compounds of androsterone glucuronide and androsterone sulfate.
16 totally different glucuronide and 29 sulfate conjugated metabolites of anabolic androgenic steroids (AAS), out there both as pure reference supplies, or synthesized/extracted from administration research, had been derivatized with GRT and their product ion spectra are offered. Product ion spectra embrace in all instances excessive variety of product ions that in some instances are attribute for sure constructions of the steroid spine.
Extra particularly, preliminary outcomes have proven main variations in fragmentation sample for 17α/17β-isomers of the sulfate-conjugates, however restricted differentiation for 17α/17β-isomers of glucuronide-conjugates and for 3α/3β- and 5α/5β-stereoisomers of each sulfate and glucuronide conjugates. Additional to the suggestion of the present work, utility on mesterolone administration research confirmed -according to the WADA TD2015IDCR- the presence of seven intact part II metabolites, one glucuronide and 6 sulfates with use of LC-ESI-MS/(MS).

Dedication of nitrofuran metabolites in honey utilizing a brand new derivatization reagent, magnetic solid-phase extraction and LC-MS/MS

On this research, 5-nitro-2-furaldehyde (5-NFA) was proposed as a brand new derivatizing agent for nitrofuran metabolites. It reacts with nitrofuran metabolites producing the guardian nitrofurans (furazolidone, furaltadone, nitrofurantoin, and nitrofurazone). Magnetic hypercrosslinked polystyrene (HCP/Fe3O4) was first used for magnetic stable part extraction (MSPE) clean-up earlier than the dedication of nitrofuran metabolite derivatives in honey by way of LC-MS/MS.
Primary parameters affecting the derivatization and MSPE effectivity had been investigated intimately and the optimum situations had been discovered. The tactic was validated utilizing honey spiked with the 4 metabolites at 1, 2 and 200 μg kg-1. Recoveries of >85% had been achieved for the all analytes. The matrix calibration curve was fitted with the correlation coefficient (R2) > 0.99 within the vary of 1-200 μg kg-1.
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Precision values expressed as relative commonplace deviation (RSD) had been <12% and <15% for intra-day and inter-day precision, respectively. The boundaries of detection (LODs) for the nitrofuran metabolites had been of 0.1-0.Three μg kg-1 and the bounds of quantitation (LOQs) had been of 0.3-1.Zero μg kg-1. The proposed LC-MS/MS technique was utilized to the evaluation of actual honey samples.

Peptide Bond Formation of Amino Acids by Transient Masking with Silylating Reagents

A one-pot peptide bond-forming response has been developed utilizing unprotected amino acids and peptides. Two totally different silylating reagents, HSi[OCH(CF3)2]3 and MTBSTFA, are instrumental for the profitable implementation of this strategy, getting used for the activation and transient masking of unprotected amino acids and peptides at C-termini and N-termini, respectively.
Moreover, CsF and imidazole are used as catalysts, activating HSi[OCH(CF3)2]3 and in addition accelerating chemoselective silylation. This technique is flexible because it tolerates facet chains that bear a variety of useful teams, whereas offering as much as >99% yields of corresponding peptides with none racemization or polymerization.

Hydrothermal Crystallization of Bismuth Oxychlorides (BiOCl) Utilizing Completely different Form Management Reagents

Bismuth oxychloride photocatalysts had been obtained utilizing solvothermal synthesis and totally different components (CTAB-cetyltrimethylammonium bromide, CTAC-cetyltrimethylammonium chloride, PVP-polyvinylpyrrolidone, SDS-sodium dodecylsulphate, U-urea and TU-thiourea). The impact of the beforehand talked about compounds was analyzed making use of structural (main crystallite measurement, crystal part composition, and so forth.), morphological (particle geometry), optical (band hole vitality) parameters, floor associated properties (floor atoms’ oxidation states), and the resulted photocatalytic exercise.
A powerful dependency was discovered between the floor pressure of the synthesis options and the general morpho-structural parameters. The primary discovering was that the traits of the semiconductors could be tuned by modifying the floor pressure of the synthesis combination. It was noticed after the photocatalytic degradation, that the white semiconductor turned to gray. Moreover, we tried to clarify the grey shade of BiOCl catalysts after the photocatalytic decompositions by Raman and XPS research.

Difluorocarbene-Mediated Cascade Cyclization: The Multifunctional Function of Ruppert-Prakash Reagent

A difluorocarbene-mediated cascade cyclization response for fast entry to gem-difluorinated 3-coumaranone derivatives was developed. The difluorocarbene acts as a bipolar CF2 constructing block, which permits a homologation cyclization course of by way of sequentially reacting with the phenolate and the ester group on the identical substrate.

FBS, Transfection Optimized

F0640-050 500ml
EUR 902.4

EL Transfection Reagent

20-abx098880
  • EUR 460.80
  • EUR 644.40
  • 0.75 ml
  • 1.5 ml

LP4K Transfection Reagent

LP4K 1.0 ml / vial
EUR 364.8
Description: Lipid based transfection reagent for large plasmid and multiple plasmid transfection in both adhesive and suspenstion cell types.

PureFection Transfection Reagent

LV750A-1 1 ml
EUR 430.8

ExFect2000 Transfection Reagent

T202-01 0.5 ml
EUR 272.4

ExFect2000 Transfection Reagent

T202-02 1 ml
EUR 379.2

ExFect2000 Transfection Reagent

T202-03 5 ml
EUR 1262.4

HighGene transfection reagent

RM09014 1000μl
EUR 324

Convoy? Transfection Reagent

1110-1ml each
EUR 409.2

CryoProtX Mix Reagents 1.5 mL

M-MDSR-61 1.5 ml ml
EUR 37
Description: CryoProtX Mix Reagents 1.5 mL

Set of 10 Biolipidure Reagents

Biolipidure-set 10mLx10
EUR 1820.4
Description: Set of 10 Biolipidure Reagents, whose applications include Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Lentifectin (TM) Transfection Reagent

DNAF003 1ml
EUR 334.57

Sapphire Insect Transfection Reagent

ABP-BVD-10003 75ul, 25 transfections Ask for price

iMFectin DNA Transfection Reagent

I7100-100 100ul
EUR 176.4

iMFectin DNA Transfection Reagent

I7100-101 1ml
EUR 530.4

iMFectin DNA Transfection Reagent

I7100-105 5x1ml
EUR 2247.6

iMFectin DNA Transfection Reagent

I7100-120 20ml
EUR 8144.4

Protoplast Solution for Transfection

GR103052 10 mL
EUR 142.8

Calcium Phosphate Transfection Kit

GR103070 100 preps
EUR 262.8

PureFection™ Transfection reagent

LV750A-5 5 ml
EUR 1639.2

GeneGlide? DNA Transfection Reagent

M1080-1000 each
EUR 636

GeneGlide? DNA Transfection Reagent

M1080-300 each
EUR 322.8

GeneGlide? DNA Transfection Reagent

M1080-500 each
EUR 433.2

GeneGlide? siRNA Transfection Reagent

M1081-1000 each
EUR 534

GeneGlide? siRNA Transfection Reagent

M1081-300 each
EUR 318

GeneGlide? siRNA Transfection Reagent

M1081-500 each
EUR 364.8

Bradford(Coomassie) Protein Assay Plus Reagents

P7201-050 450ml
EUR 225.6

Free Chlorine Reagents 0-5mg/L

WAT1028 EACH
EUR 35.11

CryoProtX Mix Eco Reagents 1.5 mL

M-MDSR-61-ECO 1.5 ml ml
EUR 37
Description: CryoProtX Mix Eco Reagents 1.5 mL

Basic Cytotoxicity Test Kits

CT17001 125 Tests
EUR 490.8

Basic Cytotoxicity Test Kits

CT17002 250 Tests
EUR 780

Total Cytotoxicity Test Kits

CT17003 125 Tests
EUR 615.6

Total Cytotoxicity Test Kits

CT17004 250 Tests
EUR 975.6

Necrosis vs Apoptosis Kits

KF17371 50-100 Tests
EUR 522

Necrosis vs Apoptosis Kits

KF17372 100-200 Tests
EUR 921.6

Pipette Stand Starter Kits

P7700-S1 1 PC
EUR 609.4

Pipette Stand Starter Kits

P7700-S2 1 PC
EUR 631.32

T&A Cloning Kits

FYC001-20P 1 vial Ask for price

Sealed Vials Calibration Kits

2659505 EACH
EUR 814.6

Carcass Sponge Sampling Kits

SWA1038 PK95
EUR 332.88

Exo-Fect Exosome Transfection Kit

EXFT10A-1 10 reactions
EUR 351.6

Exo-Fect Exosome Transfection Kit

EXFT20A-1 20 reactions
EUR 586.8

iMFectin Poly DNA Transfection Reagent

I7200-100 100ul
EUR 176.4

iMFectin Poly DNA Transfection Reagent

I7200-101 1ml
EUR 530.4

iMFectin Poly DNA Transfection Reagent

I7200-105 5x1ml
EUR 2247.6

iMFectin Poly DNA Transfection Reagent

I7200-120 20ml
EUR 8144.4

iMFectin 2000 DNA Transfection Reagent

I7300-101 1ml Ask for price

iMFectin 2000 DNA Transfection Reagent

I7300-105 5x1ml Ask for price

Phosphate-buffered Sucrose for Transfection

GR103050 1 L
EUR 142.8

Plant Electroporation Buffer for Transfection

GR103051 1 L
EUR 142.8

Transfectamine™ mRNA Transfection Reagent

60030 500 ul
EUR 203

MitoPT™ TMRE & TMRM Kits

KF17358 500 Test
EUR 574.8

MitoPT™ TMRE & TMRM Kits

KF17359 500 Test
EUR 574.8

HISTAR DETECTION SYSTEM Kits (OKSA11259)

OKSA11259 50 Tests
EUR 678
Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity:

HISTAR DETECTION SYSTEM Kits (OKSA11260)

OKSA11260 150 Tests
EUR 1602
Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity:

HISTAR DETECTION SYSTEM Kits (OKSA11261)

OKSA11261 500 Tests
EUR 2422.8
Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity:

Rat Neural Stem Cell Kits

PC37123 1 X 10(6) *minimum order 6 vials*
EUR 1148.4

Transfection-ready Cas9 SmartNuclease AAVS1 gRNA

CAS520A-1 10 ug
EUR 404.4

Exo-Fect siRNA/miRNA Transfection Kit

EXFT200A-1 20 reactions
EUR 574.8

RNAfection, RNA transfection reagent 50 ul

MR750A-1 50 ul
EUR 252

T-Pro P-Fect Transfection Reagent

JT97-N005M 1.0ml/vial
EUR 266.4

Transfection Collection™ : PDE4D Transient Pack

79286 500 rxns.
EUR 845
Description: The PDE4D Transient Pack is designed to provide the tools necessary for transiently transfecting and for screening inhibitors of PDE4D7 in cultured HEK293 cells._x000D_The assay is based on transfecting cells with the CRE luciferase reporter. CRE reporter contains the firefly luciferase gene under the control of cAMP response element (CRE). Elevation of intracellular cAMP activates CRE binding protein (CREB) to bind CRE and induce the expression of luciferase. Forskolin is commonly used to raise the intracellular level of cAMP in cell physiology studies. When cells transiently transfected with CRE reporter are activated by forskolin, the intracellular level of cAMP is upregulated, which induces the expression of CRE luciferase reporter._x000D_However, when cells are co-transfected with PDE4D7 expression vector and CRE reporter, the level of forskolin-induced cAMP is reduced, resulting in lower expression level of luciferase. When cells are treated with PDE4D inhibitor to inhibit PDE4D7 activity, cAMP level is restored, resulting in higher luciferase activity. The PDE4D Transient Pack contains transfection-ready CRE luciferase reporter. This reporter contains transfection-ready vectors for PDE4D7 expression and a CRE vector containing firefly luciferase as a cAMP pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control._x000D_It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells. The key to the PDE4D Cell-Based Activity Reporter Detection Valuepack is the CRE luciferase reporter vector, which is a cAMP/PKA Cell Signaling Pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized cAMP response elements (CRE) located upstream of a minimal promoter. The vector is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The pack also includes the PDE4D7 expression vector, and the adenylate cyclase activator, forskolin. Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 and HeLa cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep._x000D_Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293 or HeLa, but an alternate cell culture medium may be required for optimal cell growth

CellQuanti-Blue Cell Viability Assay Kits

CQBL-05K 5000
EUR 265.2
Description: Homogeneous assay for cell viability, proliferation, cytotoxcity, high-throµghput screening for anticancer agents. Fluorimetric method (530nm/590nm). Kit size: 5000 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C.

CellQuanti-Blue Cell Viability Assay Kits

CQBL-10K 10000
EUR 408
Description: Homogeneous assay for cell viability, proliferation, cytotoxcity, high-throµghput screening for anticancer agents. Fluorimetric method (530nm/590nm). Kit size: 10000 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C.

CellQuanti-MTT Cell Viability Assay Kits

CQMT-500 500
EUR 252
Description: Colorimetric (570nm) assay for cell viability, proliferation, cytotoxcity, HTS for anticancer agents. Kit size: 500 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4, -20°C.

MITOCHONDRIAL MEMBRANE POTENTIAL KIT Kits (OKSA11298)

OKSA11298 500 Tests
EUR 1147.2
Description: Description of target: MITOCHONDRIAL MEMBRANE POTENTIAL KIT ;Species reactivity: Human;Application: FC, IF;Assay info: ;Sensitivity:

MITOCHONDRIAL MEMBRANE POTENTIAL KIT Kits (OKSA11300)

OKSA11300 500 Tests
EUR 1147.2
Description: Description of target: MITOCHONDRIAL MEMBRANE POTENTIAL KIT ;Species reactivity: Human;Application: FC, IF;Assay info: ;Sensitivity:

Amersham ECL Western Blotting Detection Reagents for 4000cm2 membrane

RPN2106 EACH
EUR 314.64

Amersham ECL Western Blotting Detection Reagents for 1000cm2 membrane

RPN2109 EACH
EUR 158.46

Amersham ECL Western Blotting Detection Reagents for 6000cm2 membrane

RPN2134 1KIT
EUR 482.22

Amersham ECL Western Blotting Detection Reagents for 2000cm2 membrane

RPN2209 EACH
EUR 245.1

Transfection-ready Cas9 SmartNuclease mRNA (Eukaryotic version)

CAS500A-1 20 ug
EUR 534

50ml TC Tubes, Conical, 440 units/box

04-5540150 440 units/box
EUR 85.2

GMP IL4, 50µg

04-GMP-HU-IL4-50UG 50µg
EUR 579.6
Description: Recombinant Human IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton. The rHuIL-4 is purified by proprietary chromatographic techniques.

rHu IL 2 , 3MIU

04-RHIL2-02F01 1 vial
EUR 298.8
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.

rHu IL 2 , 3MIU , Lot 200908F02

04-RHIL2-08F02 1 vial
EUR 298.8
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.

PRE-GMP rHu GM-CSF, Molgramostim-Leukoma

04-RHUGM-CSF-7A10 300 µg
EUR 462
Description: Recombinant human GM-CSF produced in E.coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids, two pairs of disulfide bonds and having a molecular mass of approximately 14.5kD.

Monkeypox Virus Real Time PCR Kit

ZD-0076-01 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids. The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long. The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of the Monkeypox Virus DNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channel 530nm with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the 560nm fluorescence of the internal control (IC). An external positive control defined as 1×10^7 copies/ml is supplied which allow the determination of the gene load.

Monkeypox Virus Real Time PCR Kit

ZD-0076-02 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids.The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long.The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of theMonkeypox VirusDNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channelFAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load.

Mouse Anti TNP Immunotoxicity

198-TNPG-1 100 µL
EUR 469.2

Exo-Check Exosome Antibody Array

322-EXO-FBS-50A-1 100 µg
EUR 469.2

1-Step Polymorphs, Human Cell Separation

71-AN221725 1
EUR 238.8

QuantiChrom Hemoglobin Assay Kit

65-DIHB-250 250 tests
EUR 473

Porcine Parvovirus Antibody Elisa Test Kit

767-LSY-30009 192 Wells/kit
EUR 382

Rye Agar A

765-M1854-500G 500 g
EUR 82

Rye Agar B

765-M1855-500G 500 g
EUR 93

SDS-Blue™ - Coomassie based solution for protein staining in SDS-PAGE

04-GSB
  • EUR 144.00
  • EUR 96.00
  • 1L
  • 500mL
Description: SDS-Blue™ is an innovative patented formula, based on Coomassie blue, that comes in a convenient ready to use format for staining proteins in SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis). The formulation of SDS-Blue™ provides numerous advantages compared to the classic Coomassie staining or to other similar protein stains. SDS-Blue™ provides higher sensitivity, virtually no background and eliminates the need for destaining of the gel due to its high specificity and affinity to bind to protein only. Not only does SDS-Blue™ yield clear and sharp bands, but it also contains no methanol and acetic acid, making it non-hazardous, safe to handle and friendly to the environment when disposed of. Two other advantages that make SDS-Blue™ the better option is that it is not light sensitive and can be stored at ambient temperature for 24 months. And this provides a considerable convenience, especially to laboratories that need and keep big amount of protein staining solutions – no more jammed refrigerators, you can keep SDS-Blue™ wherever it is most convenient for You!

Gentodenz

19-DENZ-500 500 g
EUR 416

EasyNat Quattro

UC0104 4-module system
EUR 12000

EasyNat Octo

UC0108 8-module system
EUR 22000

Malachite Green Phosphate Assay kit

65-POMG-25H 2500 tests
EUR 333

Multiplex ELISA Kits for IL1α, IL1β, IL6, IL8, GMCSF, INF-γ, MCAF and TNFα, human

LF-EK61000 1×96T
EUR 1752

JBScreen Membrane 1-4 - All 4 kits

M-CS-309 4 Kits
EUR 1109
Description: JBScreen Membrane 1-4 - All 4 kits

Blocking Buffer 4 (Multiple Epigenetics Assay Kits)

52100 50 ml
EUR 95
Description: This Blocking Buffer 4 is optimized for minimizing background in various chemiluminescent methyltransferase, demethylase, and TET assays.

Blocking Buffer 1 (Various Epigenetics Assay Kits)

79556 50 ml
EUR 100
Description: This Blocking Buffer 1 is optimized for minimizing background in various BPS Bioscience assay kits. The BPS Bioscience Blocking Buffer improves S/B ratio of HMTs NSD2 and SetDB2 ELISAs with respect to competitor blocking buffer by both decreasing background (0% Activity control, no enzyme) and increasing assay signal (100% Activity control, enzyme without inhibitor).

Blocking Buffer 2 (BPS Immunotherapy Assay Kits)

79728 50 ml
EUR 110

Blocking Buffer 3 (Multiple PARP Assay Kits)

79743 25 ml x 2
EUR 105
Description: This Blocking Buffer 3 is optimized for minimizing background in multiple PARP BPS Bioscience Assay Kits. _x000D__x000D_

Streptavidin-HRP (For PARP & Cytokine Assay Kits)

80611 100 µl
EUR 100
Description: Streptavidin-HRP is useful in select BPS Bioscience assay kits.

100 x DEAE-dextran Stock Solution for Transfection

GR103049 10 x 10 mL
EUR 142.8

T-Pro Nonliposomal Transfection Reagent I (NTR I)

JT97-N001M 1.0ml/vial
EUR 224.4

T-Pro Nonliposomal Transfection Reagent II (NTR II)

JT97-N002M 1.0ml/vial
EUR 244.8

T-Pro Nonliposomal Transfection Reagent III (NTR III)

JT97-N006M 1.0ml/vial
EUR 266.4

Nori® Porcine 3-Plex ELISA Kits-Interferons

GR224009 96-well
EUR 603.6

Nori® Porcine 4-Plex ELISA Kits-Immunoglobulins

GR224017 96-well
EUR 654

Nori® Porcine 6-Plex ELISA Kits-BMP

GR224025 96-well
EUR 754.8

Nori® Porcine 6-Plex ELISA Kits-CCL

GR224026 96-well
EUR 754.8

Nori® Porcine 6-Plex ELISA Kits-MMP

GR224027 96-well
EUR 754.8

Nori® Porcine 6-Plex ELISA Kits-TLR

GR224028 96-well
EUR 754.8

Nori® Porcine 6-Plex ELISA Kits-Hormones

GR224029 96-well
EUR 754.8

Nori® Porcine 6-Plex ELISA Kits-SAA

GR224030 96-well
EUR 754.8

Nori® Porcine 6-Plex ELISA Kits-S100

GR224031 96-well
EUR 754.8

Nori® Porcine 6-Plex ELISA Kits-CXCL

GR224032 96-well
EUR 754.8

Nori® Equine 3-Plex ELISA Kits-Interferons

GR225009 96-well
EUR 603.6

Nori® Equine 4-Plex ELISA Kits-Immunoglobulins

GR225017 96-well
EUR 654

Nori® Equine 6-Plex ELISA Kits-BMP

GR225025 96-well
EUR 754.8

Nori® Equine 6-Plex ELISA Kits-CCL

GR225026 96-well
EUR 754.8

Nori® Equine 6-Plex ELISA Kits-MMP

GR225027 96-well
EUR 754.8

Nori® Equine 6-Plex ELISA Kits-TLR

GR225028 96-well
EUR 754.8

Nori® Equine 6-Plex ELISA Kits-Hormones

GR225029 96-well
EUR 754.8

Nori® Equine 6-Plex ELISA Kits-SAA

GR225030 96-well
EUR 754.8

Nori® Equine 6-Plex ELISA Kits-S100

GR225031 96-well
EUR 754.8

Nori® Equine 6-Plex ELISA Kits-CXCL

GR225032 96-well
EUR 754.8

Nori® Canine 3-Plex ELISA Kits-Interferons

GR226009 96-well
EUR 603.6

Nori® Canine 4-Plex ELISA Kits-Immunoglobulins

GR226017 96-well
EUR 654

Nori® Canine 6-Plex ELISA Kits-BMP

GR226025 96-well
EUR 754.8

Nori® Canine 6-Plex ELISA Kits-CCL

GR226026 96-well
EUR 754.8

Nori® Canine 6-Plex ELISA Kits-MMP

GR226027 96-well
EUR 754.8

Nori® Canine 6-Plex ELISA Kits-TLR

GR226028 96-well
EUR 754.8

Nori® Canine 6-Plex ELISA Kits-Hormones

GR226029 96-well
EUR 754.8

Nori® Canine 6-Plex ELISA Kits-SAA

GR226030 96-well
EUR 754.8

Nori® Canine 6-Plex ELISA Kits-S100

GR226031 96-well
EUR 754.8

Nori® Canine 6-Plex ELISA Kits-CXCL

GR226032 96-well
EUR 754.8

Nori® Bovine 3-Plex ELISA Kits-Interferons

GR228009 96-well
EUR 603.6

Nori® Bovine 4-Plex ELISA Kits-Immunoglobulins

GR228017 96-well
EUR 654

Nori® Bovine 6-Plex ELISA Kits-BMP

GR228025 96-well
EUR 754.8

Nori® Bovine 6-Plex ELISA Kits-CCL

GR228026 96-well
EUR 754.8

Nori® Bovine 6-Plex ELISA Kits-MMP

GR228027 96-well
EUR 754.8

Nori® Bovine 6-Plex ELISA Kits-TLR

GR228028 96-well
EUR 754.8

Nori® Bovine 6-Plex ELISA Kits-Hormones

GR228029 96-well
EUR 754.8

Nori® Bovine 6-Plex ELISA Kits-SAA

GR228030 96-well
EUR 754.8

Nori® Bovine 6-Plex ELISA Kits-S100

GR228031 96-well
EUR 754.8

Nori® Bovine 6-Plex ELISA Kits-CXCL

GR228032 96-well
EUR 754.8

Precellys 2mL Lysing Kits - Soft tissue Starter box

HOM1060 PK40
EUR 194.94

Precellys 2mL Lysing Kits - Micro-organism Starter box

HOM1062 PK40
EUR 194.94

Transfection-ready Cas9 SmartNickase mRNA (Eukaryotic Nickase mutant version)

CAS504A-1 20 ug
EUR 534

NHEJ Based HeLa Requires I-Sce1 Transfection (50ug DNA)

DR5001-HNHela-Sce 50ug DNA
EUR 3174

NHEJ Based HeLa Requires I-Sce1 Transfection (100ug DNA)

DR5001-HNHela-Sce2 100ug DNA
EUR 3486

NHEJ Based HeLa Requires I-Sce1 Transfection (250ug DNA)

DR5001-HNHela-Sce3 250ug DNA
EUR 4110

Transfection Collection™ : AP1 Transient Pack (JNK Signaling Pathway)

79266 500 rxns.
EUR 715
Description: The AP1 Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the JNK signaling pathway and the transcriptional activity of AP1 in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a JNK pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the AP1 Transient Pack is the AP1 luciferase reporter vector. This reporter contains the firefly luciferase gene under the control of multimerized AP1 responsive elements located upstream of a minimal promoter. The AP1 reporter is premixed with a constitutively-expressing Renilla luciferase vector that serves as an internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively- expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 and HeLa cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. _x000D_*Note: the kit may be used with other cell lines than HEK293 or HeLa, but an alternate cell culture medium may be required for optimal cell growth

Transfection Collection™ : Myc Transient Pack (Myc Signaling Pathway)

79284 500 rxns.
EUR 750
Description: The Myc Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the Myc signaling pathway in cultured HCT116 cells. The Myc Transient Pack contains transfection-ready vectors containing firefly luciferase as a Myc pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HCT116 cells._x000D_.The key to the Myc Transient Pack is the expression vectors for c-Myc and Myc luciferase reporter vector. Inside the cells, c-Myc will bind to Max, translocate to the nucleus, and induce expression of the Myc luciferase reporter vector. This reporter contains the firefly luciferase gene under the control of multimerized Myc responsive elements located upstream of a minimal promoter. The Myc reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. This negative control is critical for determining pathway-specific effects and background luciferase activity. An "empty" expression vector without the c-Myc gene is also provided as an additional negative control._x000D_Additionally, the pack includes cell culture medium that has been optimized for use with HCT116 cells*. HCT116 is a human colon cancer cell line with a mutated β -catenin which leads to the accumulation of β-catenin and constitutive activation of the Myc signaling pathway. This medium includes 10% fetal bovine serum, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HCT116, but an alternate cell culture medium may be required for optimal cell growth. For HEK293 cells, we recommend using BPS Medium 1 (BPS Bioscience, #79259).

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)

CAS510A-KIT 1 Kit
EUR 966

Mast/stem Cell Growth Factor Receptor Kit (Kits) Antibody

20-abx116620
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Rabbit ANTI 14-3-3 ISOFORM PANEL Kits (OKSA11258)

OKSA11258 1 u
EUR 1483.2
Description: Description of target: RABBIT ANTI 14-3-3 ISOFORM PANEL;Species reactivity: Sheep;Application: ELISA, IHC-FFPE, WB;Assay info: ;Sensitivity:

Nori® Porcine 6-Plex ELISA Kits-Growth Factors

GR224024 96-well
EUR 754.8

Nori® Porcine 6-Plex ELISA Kits-Inflammation Panel

GR224033 96-well
EUR 754.8

Nori® Porcine 6-Plex ELISA Kits-Cardiac Panel

GR224034 96-well
EUR 754.8

Nori® Equine 6-Plex ELISA Kits-Growth Factors

GR225024 96-well
EUR 754.8

Nori® Equine 6-Plex ELISA Kits-Inflammation Panel

GR225033 96-well
EUR 754.8

Nori® Equine 6-Plex ELISA Kits-Cardiac Panel

GR225034 96-well
EUR 754.8

Nori® Canine 6-Plex ELISA Kits-Growth Factors

GR226024 96-well
EUR 754.8

Nori® Canine 6-Plex ELISA Kits-Inflammation Panel

GR226033 96-well
EUR 754.8

Nori® Canine 6-Plex ELISA Kits-Cardiac Panel

GR226034 96-well
EUR 754.8

Nori® Bovine 6-Plex ELISA Kits-Growth Factors

GR228024 96-well
EUR 754.8

Nori® Bovine 6-Plex ELISA Kits-Inflammation Panel

GR228033 96-well
EUR 754.8

Nori® Bovine 6-Plex ELISA Kits-Cardiac Panel

GR228034 96-well
EUR 754.8

TruStrip WBV Tubulin protein quantitation & Western Blot Validation Kit (contains actin strips, primary antibodies and Western reagents 100 strips or 10 miniblot)

WBTUB100-K 1 Kit
EUR 634.8

HR based Kit in HeLa Requiring CRISPR Transfection (50ug DNA)

DR3000-HRHeLa-CPR 50ug DNA
EUR 3298.8

HR based Kit in HeLa Requiring CRISPR Transfection (100ug DNA)

DR3000-HRHeLa-CPR2 100ug DNA
EUR 3610.8

HR based Kit in HeLa Requiring CRISPR Transfection (250ug DNA)

DR3000-HRHeLa-CPR3 250ug DNA
EUR 4234.8

"N" SARS-CoV-2 Nucleocapsid mRNA (10 µg, Transfection-ready)

CVD19-420A-1 10 µg
EUR 328

"N" SARS-CoV-2 Nucleocapsid mRNA (20 µg, Transfection-ready)

CVD19-425A-1 20 µg
EUR 468

The Transfection Collection™ : GAL4 Transient Pack Glucocorticoid Receptor Pathway

79265 500 rxns.
EUR 715
Description: The GAL4 Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the glucocorticoid signaling pathway in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a glucocorticoid pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the GAL4 Transient Pack is the expression vector for the glucocorticoid receptor ligand binding domain that is fused to the DNA binding domain (DBD) of GAL4 (GAL4 DBD-GR). This fusion construct activates firefly luciferase expression under the control of a multimerized GAL4 upstream activation sequence (UAS). This allows for specific detection of glucocorticoid-induced activation of the glucocorticoid receptor without the need for individual transcriptional targets and with low cross-reactivity for other nuclear receptor pathways. The GAL4/UAS reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway-specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth.

Transfection Collection™ : SRE Transient Pack (MAPK/ERK Signaling Pathway)

79271 500 rxns.
EUR 750
Description: The SRE Reporter Kit Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the ERK signaling pathway and the transcriptional activity of SRE in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a MAPK/ERK pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the SRE Transient Pack is the SRE luciferase reporter vector, which is an ERK pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized SRE responsive elements located upstream of a minimal promoter. The SRE reporter is premixed with a constitutively-expressing Renilla luciferase vector that serves as an internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively- expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_* Other mammalian cell lines may also be used, but an alternate cell culture medium may be required for optimal cell growth.

Transfection Collection™ : SBE Transient Pack (TGFβ/SMAD Signaling Pathway)

79272 500 rxns.
EUR 750
Description: The SBE Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of TGFβ/SMAD signaling pathway in the cultured cells. The kit contains transfection-ready vectors containing firefly luciferase as a TGFβ/SMAD pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the SBE Transient Pack is the SBE luciferase reporter vector, which is a TGFβ pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized SBE responsive element located upstream of a minimal promoter. The SBE reporter is premixed with constitutively expressing Renilla luciferase vector that serves as internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. _x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth.

Transfection Collection™ : Notch1/CSL Transient Pack (Notch Signaling Pathway)

79285 500 rxns.
EUR 715
Description: The Notch1/CSL Transient Packis designed to provide the tools necessary for transiently transfecting and monitoring the activity of the Notch signaling pathway in HEK293 cultured cells. The kit contains transfection-ready vectors containing firefly luciferase as a Notch pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the Notch1/CSL Transient Pack is the expression vector for NOTCH1 that has a deletion of the entire extracellular domain (Notch1DE). Inside the cells, the NOTCH1 DE can be cleaved by γ-secretase and active NOTCH1 NICD is released into the nucleus. The kit also contains CSL (CBF1/RBP-Jk) luciferase reporter vector, which is a Notch pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized CSL responsive elements upstream of a minimal promoter. The CSL (CBF1/RBP-Jk) reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. _x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth,

cAMP Biotrak(TM) EIA; (Non-Acetylation Protocol; Lysis Reagents not Included); Cytiva; RPN2251

GERPN2251 EACH
EUR 551.76

Nori® Porcine 3-Plex ELISA Kits-IL1A-IL8-TNFA

GR224001 96-well
EUR 603.6

Nori® Porcine 3-Plex ELISA Kits-IL1B-IL8-TNFA

GR224002 96-well
EUR 603.6

Nori® Porcine 3-Plex ELISA Kits-IL1B-IL6-TNFA

GR224003 96-well
EUR 603.6

Nori® Porcine 3-Plex ELISA Kits-IL1B-IL10-TNFA

GR224004 96-well
EUR 603.6

Nori® Porcine 3-Plex ELISA Kits-IL6-IL8-TNFA

GR224005 96-well
EUR 603.6

Nori® Porcine 3-Plex ELISA Kits-IL6-IL10-TNFA

GR224006 96-well
EUR 603.6

Nori® Porcine 3-Plex ELISA Kits-IL1B-IL2-IL4

GR224008 96-well
EUR 603.6

Nori® Equine 3-Plex ELISA Kits-IL1A-IL8-TNFA

GR225001 96-well
EUR 603.6

Nori® Equine 3-Plex ELISA Kits-IL1B-IL8-TNFA

GR225002 96-well
EUR 603.6

Nori® Equine 3-Plex ELISA Kits-IL1B-IL6-TNFA

GR225003 96-well
EUR 603.6

Nori® Equine 3-Plex ELISA Kits-IL1B-IL10-TNFA

GR225004 96-well
EUR 603.6

Nori® Equine 3-Plex ELISA Kits-IL6-IL8-TNFA

GR225005 96-well
EUR 603.6

Nori® Equine 3-Plex ELISA Kits-IL6-IL10-TNFA

GR225006 96-well
EUR 603.6

Nori® Equine 3-Plex ELISA Kits-IL1B-IL2-IL4

GR225008 96-well
EUR 603.6

Nori® Canine 3-Plex ELISA Kits-IL1A-IL8-TNFA

GR226001 96-well
EUR 603.6

Nori® Canine 3-Plex ELISA Kits-IL1B-IL8-TNFA

GR226002 96-well
EUR 603.6

Nori® Canine 3-Plex ELISA Kits-IL1B-IL6-TNFA

GR226003 96-well
EUR 603.6

Nori® Canine 3-Plex ELISA Kits-IL1B-IL10-TNFA

GR226004 96-well
EUR 603.6

Nori® Canine 3-Plex ELISA Kits-IL6-IL8-TNFA

GR226005 96-well
EUR 603.6

Nori® Canine 3-Plex ELISA Kits-IL6-IL10-TNFA

GR226006 96-well
EUR 603.6
The potential utility of this artificial strategy is demonstrated by a late-stage modification of diethylstilbestrol. Mechanistic research revealed the a number of essential roles performed by the Ruppert-Prakash reagent.

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Monoclonal Antibodies
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