This text describes the primary synthesis and utility of fluorine-18 labelled Ruppert-Prakash reagent [18F]Me3SiCF3. [18F]Me3SiCF3 was synthesized from [18F]fluoroform with radiochemical yields of 85-95% and radiochemical purities of >95% inside 20 minutes. 18F-trifluoromethylated compounds had been efficiently ready by response of [18F]Me3SiCF3 with benzaldehydes, acetophenones and benzophenones.
Intact part II steroid metabolites have poor product ion mass spectra beneath collision induced dissociation (CID) situations. Due to this fact, we current herein the liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/(MS)) habits of intact part II metabolites of oxosteroids after derivatization.
Based mostly on the truth that Girard’s reagent T (GRT), as derivatization reagent, was each handy and environment friendly by way of the enhancement within the ionization effectivity and the manufacturing of diagnostic product ions associated to the steroid moiety, the latter was ideally chosen between methoxamine and hydroxylamine upon the mannequin compounds of androsterone glucuronide and androsterone sulfate.
16 totally different glucuronide and 29 sulfate conjugated metabolites of anabolic androgenic steroids (AAS), out there both as pure reference supplies, or synthesized/extracted from administration research, had been derivatized with GRT and their product ion spectra are offered. Product ion spectra embrace in all instances excessive variety of product ions that in some instances are attribute for sure constructions of the steroid spine.
Extra particularly, preliminary outcomes have proven main variations in fragmentation sample for 17α/17β-isomers of the sulfate-conjugates, however restricted differentiation for 17α/17β-isomers of glucuronide-conjugates and for 3α/3β- and 5α/5β-stereoisomers of each sulfate and glucuronide conjugates. Additional to the suggestion of the present work, utility on mesterolone administration research confirmed -according to the WADA TD2015IDCR- the presence of seven intact part II metabolites, one glucuronide and 6 sulfates with use of LC-ESI-MS/(MS).
Dedication of nitrofuran metabolites in honey utilizing a brand new derivatization reagent, magnetic solid-phase extraction and LC-MS/MS
On this research, 5-nitro-2-furaldehyde (5-NFA) was proposed as a brand new derivatizing agent for nitrofuran metabolites. It reacts with nitrofuran metabolites producing the guardian nitrofurans (furazolidone, furaltadone, nitrofurantoin, and nitrofurazone). Magnetic hypercrosslinked polystyrene (HCP/Fe3O4) was first used for magnetic stable part extraction (MSPE) clean-up earlier than the dedication of nitrofuran metabolite derivatives in honey by way of LC-MS/MS.
Primary parameters affecting the derivatization and MSPE effectivity had been investigated intimately and the optimum situations had been discovered. The tactic was validated utilizing honey spiked with the 4 metabolites at 1, 2 and 200 μg kg-1. Recoveries of >85% had been achieved for the all analytes. The matrix calibration curve was fitted with the correlation coefficient (R2) > 0.99 within the vary of 1-200 μg kg-1.
Precision values expressed as relative commonplace deviation (RSD) had been <12% and <15% for intra-day and inter-day precision, respectively. The boundaries of detection (LODs) for the nitrofuran metabolites had been of 0.1-0.Three μg kg-1 and the bounds of quantitation (LOQs) had been of 0.3-1.Zero μg kg-1. The proposed LC-MS/MS technique was utilized to the evaluation of actual honey samples.
Peptide Bond Formation of Amino Acids by Transient Masking with Silylating Reagents
A one-pot peptide bond-forming response has been developed utilizing unprotected amino acids and peptides. Two totally different silylating reagents, HSi[OCH(CF3)2]3 and MTBSTFA, are instrumental for the profitable implementation of this strategy, getting used for the activation and transient masking of unprotected amino acids and peptides at C-termini and N-termini, respectively.
Moreover, CsF and imidazole are used as catalysts, activating HSi[OCH(CF3)2]3 and in addition accelerating chemoselective silylation. This technique is flexible because it tolerates facet chains that bear a variety of useful teams, whereas offering as much as >99% yields of corresponding peptides with none racemization or polymerization.
Hydrothermal Crystallization of Bismuth Oxychlorides (BiOCl) Utilizing Completely different Form Management Reagents
Bismuth oxychloride photocatalysts had been obtained utilizing solvothermal synthesis and totally different components (CTAB-cetyltrimethylammonium bromide, CTAC-cetyltrimethylammonium chloride, PVP-polyvinylpyrrolidone, SDS-sodium dodecylsulphate, U-urea and TU-thiourea). The impact of the beforehand talked about compounds was analyzed making use of structural (main crystallite measurement, crystal part composition, and so forth.), morphological (particle geometry), optical (band hole vitality) parameters, floor associated properties (floor atoms’ oxidation states), and the resulted photocatalytic exercise.
A powerful dependency was discovered between the floor pressure of the synthesis options and the general morpho-structural parameters. The primary discovering was that the traits of the semiconductors could be tuned by modifying the floor pressure of the synthesis combination. It was noticed after the photocatalytic degradation, that the white semiconductor turned to gray. Moreover, we tried to clarify the grey shade of BiOCl catalysts after the photocatalytic decompositions by Raman and XPS research.
Difluorocarbene-Mediated Cascade Cyclization: The Multifunctional Function of Ruppert-Prakash Reagent
A difluorocarbene-mediated cascade cyclization response for fast entry to gem-difluorinated 3-coumaranone derivatives was developed. The difluorocarbene acts as a bipolar CF2 constructing block, which permits a homologation cyclization course of by way of sequentially reacting with the phenolate and the ester group on the identical substrate.
 FBS, Transfection Optimized |
|
F0640-050 |
GenDepot |
500ml |
EUR 902.4 |
 EL Transfection Reagent |
|
20-abx098880 |
Abbexa |
|
|
|
|
 LP4K Transfection Reagent |
|
LP4K |
GenTarget |
1.0 ml / vial |
EUR 364.8 |
|
Description: Lipid based transfection reagent for large plasmid and multiple plasmid transfection in both adhesive and suspenstion cell types. |
 PureFection Transfection Reagent |
|
LV750A-1 |
SBI |
1 ml |
EUR 430.8 |
|
|
 ExFect2000 Transfection Reagent |
|
T202-01 |
Vazyme |
0.5 ml |
EUR 272.4 |
ExFect2000 Transfection Reagent |
|
T202-02 |
Vazyme |
1 ml |
EUR 379.2 |
ExFect2000 Transfection Reagent |
|
T202-03 |
Vazyme |
5 ml |
EUR 1262.4 |
 HighGene transfection reagent |
|
RM09014 |
Abclonal |
1000μl |
EUR 324 |
 Convoy? Transfection Reagent |
|
1110-1ml |
ACTGene |
each |
EUR 409.2 |
 CryoProtX Mix Reagents 1.5 mL |
|
M-MDSR-61 |
MiTeGen |
1.5 ml ml |
EUR 37 |
|
|
|
Description: CryoProtX Mix Reagents 1.5 mL |
 Set of 10 Biolipidure Reagents |
|
Biolipidure-set |
AS ONE International |
10mLx10 |
EUR 1820.4 |
|
|
|
Description: Set of 10 Biolipidure Reagents, whose applications include Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
 Transfection Reagent) Lentifectin (TM) Transfection Reagent |
|
DNAF003 |
Bio Basic |
1ml |
EUR 334.57 |
|
|
 Sapphire Insect Transfection Reagent |
|
ABP-BVD-10003 |
Allele Biotech |
75ul, 25 transfections |
Ask for price |
|
|
 iMFectin DNA Transfection Reagent |
|
I7100-100 |
GenDepot |
100ul |
EUR 176.4 |
iMFectin DNA Transfection Reagent |
|
I7100-101 |
GenDepot |
1ml |
EUR 530.4 |
iMFectin DNA Transfection Reagent |
|
I7100-105 |
GenDepot |
5x1ml |
EUR 2247.6 |
iMFectin DNA Transfection Reagent |
|
I7100-120 |
GenDepot |
20ml |
EUR 8144.4 |
 PureFection™ Transfection reagent |
|
LV750A-5 |
SBI |
5 ml |
EUR 1639.2 |
|
|
 GeneGlide? DNA Transfection Reagent |
|
M1080-1000 |
Biovision |
each |
EUR 636 |
GeneGlide? DNA Transfection Reagent |
|
M1080-300 |
Biovision |
each |
EUR 322.8 |
GeneGlide? DNA Transfection Reagent |
|
M1080-500 |
Biovision |
each |
EUR 433.2 |
 GeneGlide? siRNA Transfection Reagent |
|
M1081-1000 |
Biovision |
each |
EUR 534 |
GeneGlide? siRNA Transfection Reagent |
|
M1081-300 |
Biovision |
each |
EUR 318 |
GeneGlide? siRNA Transfection Reagent |
|
M1081-500 |
Biovision |
each |
EUR 364.8 |
 Protein Assay Plus Reagents) Bradford(Coomassie) Protein Assay Plus Reagents |
|
P7201-050 |
GenDepot |
450ml |
EUR 225.6 |
 CryoProtX Mix Eco Reagents 1.5 mL |
|
M-MDSR-61-ECO |
MiTeGen |
1.5 ml ml |
EUR 37 |
|
|
|
Description: CryoProtX Mix Eco Reagents 1.5 mL |
 Basic Cytotoxicity Test Kits |
|
CT17001 |
Neuromics |
125 Tests |
EUR 490.8 |
Basic Cytotoxicity Test Kits |
|
CT17002 |
Neuromics |
250 Tests |
EUR 780 |
 Total Cytotoxicity Test Kits |
|
CT17003 |
Neuromics |
125 Tests |
EUR 615.6 |
Total Cytotoxicity Test Kits |
|
CT17004 |
Neuromics |
250 Tests |
EUR 975.6 |
 Necrosis vs Apoptosis Kits |
|
KF17371 |
Neuromics |
50-100 Tests |
EUR 522 |
Necrosis vs Apoptosis Kits |
|
KF17372 |
Neuromics |
100-200 Tests |
EUR 921.6 |
 Exo-Fect Exosome Transfection Kit |
|
EXFT10A-1 |
SBI |
10 reactions |
EUR 351.6 |
|
|
Exo-Fect Exosome Transfection Kit |
|
EXFT20A-1 |
SBI |
20 reactions |
EUR 586.8 |
|
|
 iMFectin Poly DNA Transfection Reagent |
|
I7200-100 |
GenDepot |
100ul |
EUR 176.4 |
iMFectin Poly DNA Transfection Reagent |
|
I7200-101 |
GenDepot |
1ml |
EUR 530.4 |
iMFectin Poly DNA Transfection Reagent |
|
I7200-105 |
GenDepot |
5x1ml |
EUR 2247.6 |
iMFectin Poly DNA Transfection Reagent |
|
I7200-120 |
GenDepot |
20ml |
EUR 8144.4 |
 iMFectin 2000 DNA Transfection Reagent |
|
I7300-101 |
GenDepot |
1ml |
Ask for price |
iMFectin 2000 DNA Transfection Reagent |
|
I7300-105 |
GenDepot |
5x1ml |
Ask for price |
 Transfectamine™ mRNA Transfection Reagent |
|
60030 |
AAT Bioquest |
500 ul |
EUR 203 |
 MitoPT™ TMRE & TMRM Kits |
|
KF17358 |
Neuromics |
500 Test |
EUR 574.8 |
MitoPT™ TMRE & TMRM Kits |
|
KF17359 |
Neuromics |
500 Test |
EUR 574.8 |
) HISTAR DETECTION SYSTEM Kits (OKSA11259) |
|
OKSA11259 |
Aviva Systems Biology |
50 Tests |
EUR 678 |
|
Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity: |
) HISTAR DETECTION SYSTEM Kits (OKSA11260) |
|
OKSA11260 |
Aviva Systems Biology |
150 Tests |
EUR 1602 |
|
Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity: |
) HISTAR DETECTION SYSTEM Kits (OKSA11261) |
|
OKSA11261 |
Aviva Systems Biology |
500 Tests |
EUR 2422.8 |
|
Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity: |
 Rat Neural Stem Cell Kits |
|
PC37123 |
Neuromics |
1 X 10(6) *minimum order 6 vials* |
EUR 1148.4 |
 Transfection-ready Cas9 SmartNuclease AAVS1 gRNA |
|
CAS520A-1 |
SBI |
10 ug |
EUR 404.4 |
|
|
 Exo-Fect siRNA/miRNA Transfection Kit |
|
EXFT200A-1 |
SBI |
20 reactions |
EUR 574.8 |
 RNAfection, RNA transfection reagent 50 ul |
|
MR750A-1 |
SBI |
50 ul |
EUR 252 |
|
|
 Transfection Collection™ : PDE4D Transient Pack |
|
79286 |
BPS Bioscience |
500 rxns. |
EUR 845 |
|
Description: The PDE4D Transient Pack is designed to provide the tools necessary for transiently transfecting and for screening inhibitors of PDE4D7 in cultured HEK293 cells._x000D_The assay is based on transfecting cells with the CRE luciferase reporter. CRE reporter contains the firefly luciferase gene under the control of cAMP response element (CRE). Elevation of intracellular cAMP activates CRE binding protein (CREB) to bind CRE and induce the expression of luciferase. Forskolin is commonly used to raise the intracellular level of cAMP in cell physiology studies. When cells transiently transfected with CRE reporter are activated by forskolin, the intracellular level of cAMP is upregulated, which induces the expression of CRE luciferase reporter._x000D_However, when cells are co-transfected with PDE4D7 expression vector and CRE reporter, the level of forskolin-induced cAMP is reduced, resulting in lower expression level of luciferase. When cells are treated with PDE4D inhibitor to inhibit PDE4D7 activity, cAMP level is restored, resulting in higher luciferase activity. The PDE4D Transient Pack contains transfection-ready CRE luciferase reporter. This reporter contains transfection-ready vectors for PDE4D7 expression and a CRE vector containing firefly luciferase as a cAMP pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control._x000D_It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells. The key to the PDE4D Cell-Based Activity Reporter Detection Valuepack is the CRE luciferase reporter vector, which is a cAMP/PKA Cell Signaling Pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized cAMP response elements (CRE) located upstream of a minimal promoter. The vector is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The pack also includes the PDE4D7 expression vector, and the adenylate cyclase activator, forskolin. Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 and HeLa cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep._x000D_Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293 or HeLa, but an alternate cell culture medium may be required for optimal cell growth |
 CellQuanti-Blue Cell Viability Assay Kits |
|
CQBL-05K |
BioAssay Systems |
5000 |
EUR 265.2 |
|
Description: Homogeneous assay for cell viability, proliferation, cytotoxcity, high-throµghput screening for anticancer agents. Fluorimetric method (530nm/590nm). Kit size: 5000 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C. |
CellQuanti-Blue Cell Viability Assay Kits |
|
CQBL-10K |
BioAssay Systems |
10000 |
EUR 408 |
|
Description: Homogeneous assay for cell viability, proliferation, cytotoxcity, high-throµghput screening for anticancer agents. Fluorimetric method (530nm/590nm). Kit size: 10000 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C. |
 CellQuanti-MTT Cell Viability Assay Kits |
|
CQMT-500 |
BioAssay Systems |
500 |
EUR 252 |
|
Description: Colorimetric (570nm) assay for cell viability, proliferation, cytotoxcity, HTS for anticancer agents. Kit size: 500 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4, -20°C. |
) MITOCHONDRIAL MEMBRANE POTENTIAL KIT Kits (OKSA11298) |
|
OKSA11298 |
Aviva Systems Biology |
500 Tests |
EUR 1147.2 |
|
Description: Description of target: MITOCHONDRIAL MEMBRANE POTENTIAL KIT ;Species reactivity: Human;Application: FC, IF;Assay info: ;Sensitivity: |
) MITOCHONDRIAL MEMBRANE POTENTIAL KIT Kits (OKSA11300) |
|
OKSA11300 |
Aviva Systems Biology |
500 Tests |
EUR 1147.2 |
|
Description: Description of target: MITOCHONDRIAL MEMBRANE POTENTIAL KIT ;Species reactivity: Human;Application: FC, IF;Assay info: ;Sensitivity: |
) Transfection-ready Cas9 SmartNuclease mRNA (Eukaryotic version) |
|
CAS500A-1 |
SBI |
20 ug |
EUR 534 |
|
|
 50ml TC Tubes, Conical, 440 units/box |
|
04-5540150 |
Gentaur Genprice |
440 units/box |
EUR 85.2 |
 GMP IL4, 50µg |
|
04-GMP-HU-IL4-50UG |
Gentaur Genprice |
50µg |
EUR 579.6 |
|
Description: Recombinant Human IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton. The rHuIL-4 is purified by proprietary chromatographic techniques. |
 rHu IL 2 , 3MIU |
|
04-RHIL2-02F01 |
Gentaur Genprice |
1 vial |
EUR 298.8 |
|
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated. |
 rHu IL 2 , 3MIU , Lot 200908F02 |
|
04-RHIL2-08F02 |
Gentaur Genprice |
1 vial |
EUR 298.8 |
|
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated. |
 PRE-GMP rHu GM-CSF, Molgramostim-Leukoma |
|
04-RHUGM-CSF-7A10 |
Gentaur Genprice |
300 µg |
EUR 462 |
|
Description: Recombinant human GM-CSF produced in E.coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids, two pairs of disulfide bonds and having a molecular mass of approximately 14.5kD. |
 Monkeypox Virus Real Time PCR Kit |
|
ZD-0076-01 |
Gentaur Genprice |
25 tests/kit |
Ask for price |
|
|
|
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids. The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long. The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of the Monkeypox Virus DNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channel 530nm with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the 560nm fluorescence of the internal control (IC). An external positive control defined as 1×10^7 copies/ml is supplied which allow the determination of the gene load. |
Monkeypox Virus Real Time PCR Kit |
|
ZD-0076-02 |
Gentaur Genprice |
25 tests/kit |
Ask for price |
|
|
|
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids.The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long.The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of theMonkeypox VirusDNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channelFAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load. |
 Exo-Check Exosome Antibody Array |
|
322-EXO-FBS-50A-1 |
Gentaur Genprice |
100 µg |
EUR 469.2 |
 1-Step Polymorphs, Human Cell Separation |
|
71-AN221725 |
Gentaur Genprice |
1 |
EUR 238.8 |
 Porcine Parvovirus Antibody Elisa Test Kit |
|
767-LSY-30009 |
Gentaur Genprice |
192 Wells/kit |
EUR 382 |
 SDS-Blue™ - Coomassie based solution for protein staining in SDS-PAGE |
|
04-GSB |
Gentaur Genprice |
|
|
|
|
|
Description: SDS-Blue™ is an innovative patented formula, based on Coomassie blue, that comes in a convenient ready to use format for staining proteins in SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis). The formulation of SDS-Blue™ provides numerous advantages compared to the classic Coomassie staining or to other similar protein stains. SDS-Blue™ provides higher sensitivity, virtually no background and eliminates the need for destaining of the gel due to its high specificity and affinity to bind to protein only. Not only does SDS-Blue™ yield clear and sharp bands, but it also contains no methanol and acetic acid, making it non-hazardous, safe to handle and friendly to the environment when disposed of. Two other advantages that make SDS-Blue™ the better option is that it is not light sensitive and can be stored at ambient temperature for 24 months. And this provides a considerable convenience, especially to laboratories that need and keep big amount of protein staining solutions – no more jammed refrigerators, you can keep SDS-Blue™ wherever it is most convenient for You! |
 Malachite Green Phosphate Assay kit |
|
65-POMG-25H |
Gentaur Genprice |
2500 tests |
EUR 333 |
 Multiplex ELISA Kits for IL1α, IL1β, IL6, IL8, GMCSF, INF-γ, MCAF and TNFα, human |
|
LF-EK61000 |
Abfrontier |
1×96T |
EUR 1752 |
 JBScreen Membrane 1-4 - All 4 kits |
|
M-CS-309 |
MiTeGen |
4 Kits |
EUR 1109 |
|
|
|
Description: JBScreen Membrane 1-4 - All 4 kits |
) Blocking Buffer 4 (Multiple Epigenetics Assay Kits) |
|
52100 |
BPS Bioscience |
50 ml |
EUR 95 |
|
Description: This Blocking Buffer 4 is optimized for minimizing background in various chemiluminescent methyltransferase, demethylase, and TET assays. |
) Blocking Buffer 1 (Various Epigenetics Assay Kits) |
|
79556 |
BPS Bioscience |
50 ml |
EUR 100 |
|
Description: This Blocking Buffer 1 is optimized for minimizing background in various BPS Bioscience assay kits. The BPS Bioscience Blocking Buffer improves S/B ratio of HMTs NSD2 and SetDB2 ELISAs with respect to competitor blocking buffer by both decreasing background (0% Activity control, no enzyme) and increasing assay signal (100% Activity control, enzyme without inhibitor). |
) Blocking Buffer 2 (BPS Immunotherapy Assay Kits) |
|
79728 |
BPS Bioscience |
50 ml |
EUR 110 |
) Blocking Buffer 3 (Multiple PARP Assay Kits) |
|
79743 |
BPS Bioscience |
25 ml x 2 |
EUR 105 |
|
Description: This Blocking Buffer 3 is optimized for minimizing background in multiple PARP BPS Bioscience Assay Kits. _x000D__x000D_ |
) Streptavidin-HRP (For PARP & Cytokine Assay Kits) |
|
80611 |
BPS Bioscience |
100 µl |
EUR 100 |
|
Description: Streptavidin-HRP is useful in select BPS Bioscience assay kits. |
 100 x DEAE-dextran Stock Solution for Transfection |
|
GR103049 |
Genorise Scientific |
10 x 10 mL |
EUR 142.8 |
) T-Pro Nonliposomal Transfection Reagent I (NTR I) |
|
JT97-N001M |
T-Pro Biotechnology |
1.0ml/vial |
EUR 224.4 |
) T-Pro Nonliposomal Transfection Reagent II (NTR II) |
|
JT97-N002M |
T-Pro Biotechnology |
1.0ml/vial |
EUR 244.8 |
) T-Pro Nonliposomal Transfection Reagent III (NTR III) |
|
JT97-N006M |
T-Pro Biotechnology |
1.0ml/vial |
EUR 266.4 |
) Transfection-ready Cas9 SmartNickase mRNA (Eukaryotic Nickase mutant version) |
|
CAS504A-1 |
SBI |
20 ug |
EUR 534 |
|
|
) NHEJ Based HeLa Requires I-Sce1 Transfection (50ug DNA) |
|
DR5001-HNHela-Sce |
TopoGen |
50ug DNA |
EUR 3174 |
) NHEJ Based HeLa Requires I-Sce1 Transfection (100ug DNA) |
|
DR5001-HNHela-Sce2 |
TopoGen |
100ug DNA |
EUR 3486 |
) NHEJ Based HeLa Requires I-Sce1 Transfection (250ug DNA) |
|
DR5001-HNHela-Sce3 |
TopoGen |
250ug DNA |
EUR 4110 |
) Transfection Collection™ : AP1 Transient Pack (JNK Signaling Pathway) |
|
79266 |
BPS Bioscience |
500 rxns. |
EUR 715 |
|
Description: The AP1 Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the JNK signaling pathway and the transcriptional activity of AP1 in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a JNK pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the AP1 Transient Pack is the AP1 luciferase reporter vector. This reporter contains the firefly luciferase gene under the control of multimerized AP1 responsive elements located upstream of a minimal promoter. The AP1 reporter is premixed with a constitutively-expressing Renilla luciferase vector that serves as an internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively- expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 and HeLa cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. _x000D_*Note: the kit may be used with other cell lines than HEK293 or HeLa, but an alternate cell culture medium may be required for optimal cell growth |
) Transfection Collection™ : Myc Transient Pack (Myc Signaling Pathway) |
|
79284 |
BPS Bioscience |
500 rxns. |
EUR 750 |
|
Description: The Myc Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the Myc signaling pathway in cultured HCT116 cells. The Myc Transient Pack contains transfection-ready vectors containing firefly luciferase as a Myc pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HCT116 cells._x000D_.The key to the Myc Transient Pack is the expression vectors for c-Myc and Myc luciferase reporter vector. Inside the cells, c-Myc will bind to Max, translocate to the nucleus, and induce expression of the Myc luciferase reporter vector. This reporter contains the firefly luciferase gene under the control of multimerized Myc responsive elements located upstream of a minimal promoter. The Myc reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. This negative control is critical for determining pathway-specific effects and background luciferase activity. An "empty" expression vector without the c-Myc gene is also provided as an additional negative control._x000D_Additionally, the pack includes cell culture medium that has been optimized for use with HCT116 cells*. HCT116 is a human colon cancer cell line with a mutated β -catenin which leads to the accumulation of β-catenin and constitutive activation of the Myc signaling pathway. This medium includes 10% fetal bovine serum, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HCT116, but an alternate cell culture medium may be required for optimal cell growth. For HEK293 cells, we recommend using BPS Medium 1 (BPS Bioscience, #79259). |
) T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents) |
|
CAS510A-KIT |
SBI |
1 Kit |
EUR 966 |
|
|
 Antibody) Mast/stem Cell Growth Factor Receptor Kit (Kits) Antibody |
|
20-abx116620 |
Abbexa |
|
|
|
|
) Rabbit ANTI 14-3-3 ISOFORM PANEL Kits (OKSA11258) |
|
OKSA11258 |
Aviva Systems Biology |
1 u |
EUR 1483.2 |
|
Description: Description of target: RABBIT ANTI 14-3-3 ISOFORM PANEL;Species reactivity: Sheep;Application: ELISA, IHC-FFPE, WB;Assay info: ;Sensitivity: |
 Nori® Porcine 6-Plex ELISA Kits-Inflammation Panel |
|
GR224033 |
Genorise Scientific |
96-well |
EUR 754.8 |
 Nori® Equine 6-Plex ELISA Kits-Inflammation Panel |
|
GR225033 |
Genorise Scientific |
96-well |
EUR 754.8 |
 Nori® Canine 6-Plex ELISA Kits-Inflammation Panel |
|
GR226033 |
Genorise Scientific |
96-well |
EUR 754.8 |
 Nori® Bovine 6-Plex ELISA Kits-Inflammation Panel |
|
GR228033 |
Genorise Scientific |
96-well |
EUR 754.8 |
) TruStrip WBV Tubulin protein quantitation & Western Blot Validation Kit (contains actin strips, primary antibodies and Western reagents 100 strips or 10 miniblot) |
|
WBTUB100-K |
Alpha Diagnostics |
1 Kit |
EUR 634.8 |
) HR based Kit in HeLa Requiring CRISPR Transfection (50ug DNA) |
|
DR3000-HRHeLa-CPR |
TopoGen |
50ug DNA |
EUR 3298.8 |
) HR based Kit in HeLa Requiring CRISPR Transfection (100ug DNA) |
|
DR3000-HRHeLa-CPR2 |
TopoGen |
100ug DNA |
EUR 3610.8 |
) HR based Kit in HeLa Requiring CRISPR Transfection (250ug DNA) |
|
DR3000-HRHeLa-CPR3 |
TopoGen |
250ug DNA |
EUR 4234.8 |
 "N" SARS-CoV-2 Nucleocapsid mRNA (10 µg, Transfection-ready) |
|
CVD19-420A-1 |
SBI |
10 µg |
EUR 328 |
"N" SARS-CoV-2 Nucleocapsid mRNA (20 µg, Transfection-ready) |
|
CVD19-425A-1 |
SBI |
20 µg |
EUR 468 |
 The Transfection Collection™ : GAL4 Transient Pack Glucocorticoid Receptor Pathway |
|
79265 |
BPS Bioscience |
500 rxns. |
EUR 715 |
|
Description: The GAL4 Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the glucocorticoid signaling pathway in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a glucocorticoid pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the GAL4 Transient Pack is the expression vector for the glucocorticoid receptor ligand binding domain that is fused to the DNA binding domain (DBD) of GAL4 (GAL4 DBD-GR). This fusion construct activates firefly luciferase expression under the control of a multimerized GAL4 upstream activation sequence (UAS). This allows for specific detection of glucocorticoid-induced activation of the glucocorticoid receptor without the need for individual transcriptional targets and with low cross-reactivity for other nuclear receptor pathways. The GAL4/UAS reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway-specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth. |
) Transfection Collection™ : SRE Transient Pack (MAPK/ERK Signaling Pathway) |
|
79271 |
BPS Bioscience |
500 rxns. |
EUR 750 |
|
Description: The SRE Reporter Kit Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the ERK signaling pathway and the transcriptional activity of SRE in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a MAPK/ERK pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the SRE Transient Pack is the SRE luciferase reporter vector, which is an ERK pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized SRE responsive elements located upstream of a minimal promoter. The SRE reporter is premixed with a constitutively-expressing Renilla luciferase vector that serves as an internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively- expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_* Other mammalian cell lines may also be used, but an alternate cell culture medium may be required for optimal cell growth. |
) Transfection Collection™ : SBE Transient Pack (TGFβ/SMAD Signaling Pathway) |
|
79272 |
BPS Bioscience |
500 rxns. |
EUR 750 |
|
Description: The SBE Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of TGFβ/SMAD signaling pathway in the cultured cells. The kit contains transfection-ready vectors containing firefly luciferase as a TGFβ/SMAD pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the SBE Transient Pack is the SBE luciferase reporter vector, which is a TGFβ pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized SBE responsive element located upstream of a minimal promoter. The SBE reporter is premixed with constitutively expressing Renilla luciferase vector that serves as internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. _x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth. |
) Transfection Collection™ : Notch1/CSL Transient Pack (Notch Signaling Pathway) |
|
79285 |
BPS Bioscience |
500 rxns. |
EUR 715 |
|
Description: The Notch1/CSL Transient Packis designed to provide the tools necessary for transiently transfecting and monitoring the activity of the Notch signaling pathway in HEK293 cultured cells. The kit contains transfection-ready vectors containing firefly luciferase as a Notch pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the Notch1/CSL Transient Pack is the expression vector for NOTCH1 that has a deletion of the entire extracellular domain (Notch1DE). Inside the cells, the NOTCH1 DE can be cleaved by γ-secretase and active NOTCH1 NICD is released into the nucleus. The kit also contains CSL (CBF1/RBP-Jk) luciferase reporter vector, which is a Notch pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized CSL responsive elements upstream of a minimal promoter. The CSL (CBF1/RBP-Jk) reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. _x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth, |
 EIA; (Non-Acetylation Protocol; Lysis Reagents not Included); Cytiva; RPN2251) cAMP Biotrak(TM) EIA; (Non-Acetylation Protocol; Lysis Reagents not Included); Cytiva; RPN2251 |
|
GERPN2251 |
Scientific Laboratory Supplies |
EACH |
EUR 551.76 |
The potential utility of this artificial strategy is demonstrated by a late-stage modification of diethylstilbestrol. Mechanistic research revealed the a number of essential roles performed by the Ruppert-Prakash reagent.
