This text describes the primary synthesis and utility of fluorine-18 labelled Ruppert-Prakash reagent [18F]Me3SiCF3. [18F]Me3SiCF3 was synthesized from [18F]fluoroform with radiochemical yields of 85-95% and radiochemical purities of >95% inside 20 minutes. 18F-trifluoromethylated compounds had been efficiently ready by response of [18F]Me3SiCF3 with benzaldehydes, acetophenones and benzophenones.
Intact part II steroid metabolites have poor product ion mass spectra beneath collision induced dissociation (CID) situations. Due to this fact, we current herein the liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/(MS)) habits of intact part II metabolites of oxosteroids after derivatization.
Based mostly on the truth that Girard’s reagent T (GRT), as derivatization reagent, was each handy and environment friendly by way of the enhancement within the ionization effectivity and the manufacturing of diagnostic product ions associated to the steroid moiety, the latter was ideally chosen between methoxamine and hydroxylamine upon the mannequin compounds of androsterone glucuronide and androsterone sulfate.
16 totally different glucuronide and 29 sulfate conjugated metabolites of anabolic androgenic steroids (AAS), out there both as pure reference supplies, or synthesized/extracted from administration research, had been derivatized with GRT and their product ion spectra are offered. Product ion spectra embrace in all instances excessive variety of product ions that in some instances are attribute for sure constructions of the steroid spine.
Extra particularly, preliminary outcomes have proven main variations in fragmentation sample for 17α/17β-isomers of the sulfate-conjugates, however restricted differentiation for 17α/17β-isomers of glucuronide-conjugates and for 3α/3β- and 5α/5β-stereoisomers of each sulfate and glucuronide conjugates. Additional to the suggestion of the present work, utility on mesterolone administration research confirmed -according to the WADA TD2015IDCR- the presence of seven intact part II metabolites, one glucuronide and 6 sulfates with use of LC-ESI-MS/(MS).
Dedication of nitrofuran metabolites in honey utilizing a brand new derivatization reagent, magnetic solid-phase extraction and LC-MS/MS
On this research, 5-nitro-2-furaldehyde (5-NFA) was proposed as a brand new derivatizing agent for nitrofuran metabolites. It reacts with nitrofuran metabolites producing the guardian nitrofurans (furazolidone, furaltadone, nitrofurantoin, and nitrofurazone). Magnetic hypercrosslinked polystyrene (HCP/Fe3O4) was first used for magnetic stable part extraction (MSPE) clean-up earlier than the dedication of nitrofuran metabolite derivatives in honey by way of LC-MS/MS.
Primary parameters affecting the derivatization and MSPE effectivity had been investigated intimately and the optimum situations had been discovered. The tactic was validated utilizing honey spiked with the 4 metabolites at 1, 2 and 200 μg kg-1. Recoveries of >85% had been achieved for the all analytes. The matrix calibration curve was fitted with the correlation coefficient (R2) > 0.99 within the vary of 1-200 μg kg-1.
Precision values expressed as relative commonplace deviation (RSD) had been <12% and <15% for intra-day and inter-day precision, respectively. The boundaries of detection (LODs) for the nitrofuran metabolites had been of 0.1-0.Three μg kg-1 and the bounds of quantitation (LOQs) had been of 0.3-1.Zero μg kg-1. The proposed LC-MS/MS technique was utilized to the evaluation of actual honey samples.
Peptide Bond Formation of Amino Acids by Transient Masking with Silylating Reagents
A one-pot peptide bond-forming response has been developed utilizing unprotected amino acids and peptides. Two totally different silylating reagents, HSi[OCH(CF3)2]3 and MTBSTFA, are instrumental for the profitable implementation of this strategy, getting used for the activation and transient masking of unprotected amino acids and peptides at C-termini and N-termini, respectively.
Moreover, CsF and imidazole are used as catalysts, activating HSi[OCH(CF3)2]3 and in addition accelerating chemoselective silylation. This technique is flexible because it tolerates facet chains that bear a variety of useful teams, whereas offering as much as >99% yields of corresponding peptides with none racemization or polymerization.
Hydrothermal Crystallization of Bismuth Oxychlorides (BiOCl) Utilizing Completely different Form Management Reagents
Bismuth oxychloride photocatalysts had been obtained utilizing solvothermal synthesis and totally different components (CTAB-cetyltrimethylammonium bromide, CTAC-cetyltrimethylammonium chloride, PVP-polyvinylpyrrolidone, SDS-sodium dodecylsulphate, U-urea and TU-thiourea). The impact of the beforehand talked about compounds was analyzed making use of structural (main crystallite measurement, crystal part composition, and so forth.), morphological (particle geometry), optical (band hole vitality) parameters, floor associated properties (floor atoms’ oxidation states), and the resulted photocatalytic exercise.
A powerful dependency was discovered between the floor pressure of the synthesis options and the general morpho-structural parameters. The primary discovering was that the traits of the semiconductors could be tuned by modifying the floor pressure of the synthesis combination. It was noticed after the photocatalytic degradation, that the white semiconductor turned to gray. Moreover, we tried to clarify the grey shade of BiOCl catalysts after the photocatalytic decompositions by Raman and XPS research.
Difluorocarbene-Mediated Cascade Cyclization: The Multifunctional Function of Ruppert-Prakash Reagent
A difluorocarbene-mediated cascade cyclization response for fast entry to gem-difluorinated 3-coumaranone derivatives was developed. The difluorocarbene acts as a bipolar CF2 constructing block, which permits a homologation cyclization course of by way of sequentially reacting with the phenolate and the ester group on the identical substrate.
GeneGlide? siRNA Transfection Reagent |
M1081-1000 |
Biovision |
each |
EUR 534 |
GeneGlide? siRNA Transfection Reagent |
M1081-300 |
Biovision |
each |
EUR 318 |
GeneGlide? siRNA Transfection Reagent |
M1081-500 |
Biovision |
each |
EUR 364.8 |
GENIUS DNA Transfection Reagent - 1.0 ml |
WB7-1010 |
Westburg |
each |
EUR 244.71 |
Transfection Reagent (1 mL) |
P901 |
101Bio |
1 mL |
EUR 369 |
Transfection Reagent (0.1 mL) |
P901S |
101Bio |
0.1 mL |
EUR 119 |
Convoy? Transfection Reagent |
1110-1ml |
ACTGene |
each |
EUR 409.2 |
Transfection Reagent (in vivo) |
MBS635395-012mL |
MyBiosource |
0.12mL |
EUR 1160 |
Transfection Reagent (in vivo) |
MBS635395-5x012mL |
MyBiosource |
5x0.12mL |
EUR 5075 |
NanoFect Transfection Reagent |
NF100 |
ALSTEM |
1 ml |
EUR 189.63 |
HighGene transfection reagent |
RM09014 |
Abclonal |
1000μl |
EUR 324 |
Transfection Reagent (in vitro) |
MBS635268-1mL |
MyBiosource |
1mL |
EUR 755 |
Transfection Reagent (in vitro) |
MBS635268-5x1mL |
MyBiosource |
5x1mL |
EUR 3240 |
p-Fect, Transfection reagent |
MBS555424-5x75Reactions |
MyBiosource |
5x75Reactions |
EUR 1780 |
p-Fect, Transfection reagent |
MBS555424-75Reactions |
MyBiosource |
75Reactions |
EUR 430 |
Liposomal Transfection Reagent |
abx298031-100g |
Abbexa |
100 µg |
Ask for price |
Liposomal Transfection Reagent |
abx298031-20g |
Abbexa |
20 µg |
EUR 575 |
Liposomal Transfection Reagent |
abx298031-50g |
Abbexa |
50 µg |
EUR 875 |
Susfectin Transfection Reagent |
MBS4160451-1mL |
MyBiosource |
1mL |
EUR 270 |
Susfectin Transfection Reagent |
MBS4160451-5x1mL |
MyBiosource |
5x1mL |
EUR 960 |
GENIUS DNA Transfection Reagent-5 x WB 7-1010 (5x1.0ml) |
WB7-1050 |
Westburg |
each |
EUR 970.1 |
Convoy™ Transfection Reagent |
1110 |
ACTGene |
1ml |
EUR 251.55 |
|
n-Blast Transfection Reagent |
MBS555427-075mL |
MyBiosource |
0.75mL |
EUR 460 |
n-Blast Transfection Reagent |
MBS555427-5x075mL |
MyBiosource |
5x0.75mL |
EUR 1910 |
Transfectamineâ„¢ 5000 Transfection Reagent |
60020-05mL |
AAT Bioquest |
0.5 mL |
EUR 222 |
Description: Transfectamine™ 5000 Transfection Reagent is a powerful and versatile transfection reagent for the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells. |
Transfectamineâ„¢ 5000 Transfection Reagent |
60021-1mL |
AAT Bioquest |
1 mL |
EUR 334 |
Description: Transfectamine™ 5000 Transfection Reagent is a powerful and versatile transfection reagent for the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells. |
Transfectamineâ„¢ 5000 Transfection Reagent |
60022-5mL |
AAT Bioquest |
5 mL |
EUR 846 |
Description: Transfectamine™ 5000 Transfection Reagent is a powerful and versatile transfection reagent for the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells. |
ExFect2000 Transfection Reagent |
T202-01 |
Vazyme |
0.5 ml |
EUR 272.4 |
ExFect2000 Transfection Reagent |
T202-02 |
Vazyme |
1 ml |
EUR 379.2 |
ExFect2000 Transfection Reagent |
T202-03 |
Vazyme |
5 ml |
EUR 1262.4 |
RNAifectin Transfection Reagent |
MBS8580098-1mL |
MyBiosource |
1mL |
EUR 305 |
RNAifectin Transfection Reagent |
MBS8580098-5x1mL |
MyBiosource |
5x1mL |
EUR 1235 |
PureFection Transfection Reagent |
LV750A-1 |
SBI |
1 ml |
EUR 303 |
Lentifectin Transfection Reagent |
MBS8580099-1mL |
MyBiosource |
1mL |
EUR 290 |
Lentifectin Transfection Reagent |
MBS8580099-5x1mL |
MyBiosource |
5x1mL |
EUR 1160 |
DNAfectin 2100 Transfection Reagent |
MBS8580096-1mg |
MyBiosource |
1mg |
EUR 280 |
DNAfectin 2100 Transfection Reagent |
MBS8580096-5x1mg |
MyBiosource |
5x1mg |
EUR 1115 |
Transfectamineâ„¢ mRNA Transfection Reagent |
60030-500ul |
AAT Bioquest |
500 ul |
EUR 203 |
|
Description: Transfectamineâ„¢ mRNA Transfection Reagent is a powerful and versatile transfection reagent designed to introduce a higher amount of mRNA into eukaryotic cells, or more specifically, into animal cells. |
Transfectamineâ„¢ mRNA Transfection Reagent |
60031-5ml |
AAT Bioquest |
5 ml |
EUR 989 |
|
Description: Transfectamineâ„¢ mRNA Transfection Reagent is a powerful and versatile transfection reagent designed to introduce a higher amount of mRNA into eukaryotic cells, or more specifically, into animal cells. |
Susfectin™ Transfection Reagent |
G4000 |
ABM |
1.0 ml |
EUR 195 |
iMFectin DNA Transfection Reagent |
I7100-100 |
GenDepot |
100ul |
EUR 176.4 |
iMFectin DNA Transfection Reagent |
I7100-101 |
GenDepot |
1ml |
EUR 530.4 |
iMFectin DNA Transfection Reagent |
I7100-105 |
GenDepot |
5x1ml |
EUR 2247.6 |
iMFectin DNA Transfection Reagent |
I7100-120 |
GenDepot |
20ml |
EUR 8144.4 |
EnoGeneFec 2000 Transfection Reagent |
MBS8579497-05mL |
MyBiosource |
0.5mL |
EUR 220 |
EnoGeneFec 2000 Transfection Reagent |
MBS8579497-15mL |
MyBiosource |
1.5mL |
EUR 410 |
EnoGeneFec 2000 Transfection Reagent |
MBS8579497-1mL |
MyBiosource |
1mL |
EUR 300 |
EnoGeneFec 2000 Transfection Reagent |
MBS8579497-5x15mL |
MyBiosource |
5x1.5mL |
EUR 1685 |
EnoGeneFec 2100 Transfection Reagent |
MBS8579500-05mL |
MyBiosource |
0.5mL |
EUR 240 |
EnoGeneFec 2100 Transfection Reagent |
MBS8579500-15mL |
MyBiosource |
1.5mL |
EUR 430 |
EnoGeneFec 2100 Transfection Reagent |
MBS8579500-1mL |
MyBiosource |
1mL |
EUR 315 |
EnoGeneFec 2100 Transfection Reagent |
MBS8579500-5x15mL |
MyBiosource |
5x1.5mL |
EUR 1780 |
EnoGeneFec 2200 Transfection Reagent |
MBS8579503-05mL |
MyBiosource |
0.5mL |
EUR 255 |
EnoGeneFec 2200 Transfection Reagent |
MBS8579503-15mL |
MyBiosource |
1.5mL |
EUR 450 |
EnoGeneFec 2200 Transfection Reagent |
MBS8579503-1mL |
MyBiosource |
1mL |
EUR 335 |
EnoGeneFec 2200 Transfection Reagent |
MBS8579503-5x15mL |
MyBiosource |
5x1.5mL |
EUR 1870 |
EnoGeneFec 2300 Transfection Reagent |
MBS8579506-05mL |
MyBiosource |
0.5mL |
EUR 260 |
EnoGeneFec 2300 Transfection Reagent |
MBS8579506-15mL |
MyBiosource |
1.5mL |
EUR 460 |
EnoGeneFec 2300 Transfection Reagent |
MBS8579506-1mL |
MyBiosource |
1mL |
EUR 345 |
EnoGeneFec 2300 Transfection Reagent |
MBS8579506-5x15mL |
MyBiosource |
5x1.5mL |
EUR 1920 |
Lipomaster 2000 Transfection Reagent |
TL201-00-50ul |
Vazyme |
50 μl |
EUR 1.09 |
Lipomaster 2000 Transfection Reagent |
TL201-01-15ml |
Vazyme |
1.5 ml |
EUR 305.75 |
Lipomaster 2000 Transfection Reagent |
TL201-02-415ml |
Vazyme |
4 × 1.5 ml |
EUR 1035.5 |
Lipomaster 3000 Transfection Reagent |
TL301-00-50ul |
Vazyme |
50 μl |
EUR 1.09 |
Lipomaster 3000 Transfection Reagent |
TL301-01-15ml |
Vazyme |
1.5 ml |
EUR 414.2 |
Lipomaster 3000 Transfection Reagent |
TL301-02-315ml |
Vazyme |
3 ×1.5 ml |
EUR 912.33 |
RNAifectin™ Transfection Reagent |
E36G073 |
EnoGene |
1.0 ml (100-500 transfections) |
EUR 228.57 |
Lentifectin (TM) Transfection Reagent |
DNAF003 |
Bio Basic |
1ml |
EUR 334.57 |
|
Lentifectin™ Transfection Reagent |
E36G074 |
EnoGene |
1.0 ml |
EUR 214.29 |
DNAfectin Plus Transfection Reagent |
MBS8580097-1mL |
MyBiosource |
1mL |
EUR 345 |
DNAfectin Plus Transfection Reagent |
MBS8580097-5x1mL |
MyBiosource |
5x1mL |
EUR 1395 |
DNAfectin Plus Transfection Reagent |
MBS4160450-1mL |
MyBiosource |
1mL |
EUR 270 |
DNAfectin Plus Transfection Reagent |
MBS4160450-5x1mL |
MyBiosource |
5x1mL |
EUR 960 |
jetOPTIMUS Transfection Reagent, 1.5mL |
PO101000006 |
Westburg |
each |
EUR 1132.51 |
jetOPTIMUS Transfection Reagent, 0.1mL |
PO101000051 |
Westburg |
each |
EUR 98.1 |
DNAfectin™ 2100 Transfection Reagent |
E36G2100 |
EnoGene |
1.0 mg/1.0 ml |
EUR 200 |
baculoFECTIN II Transfection Reagent 150 ul |
GWB-300101 |
GenWay Biotech |
150 ul |
Ask for price |
i-Fect si RNA Transfection Reagent |
MBS555708-5x075mL |
MyBiosource |
5x0.75mL |
EUR 2475 |
Sapphire Insect Transfection Reagent |
ABP-BVD-10003 |
Allele Biotech |
75ul, 25 transfections |
Ask for price |
|
baculoFECTIN II transfection reagent |
GWB-300106 |
GenWay Biotech |
1 ml |
Ask for price |
|
Non-Liposomal Transfection Reagent |
abx098880-100l |
Abbexa |
100 µl |
EUR 575 |
Non-Liposomal Transfection Reagent |
abx098880-1ml |
Abbexa |
1 ml |
Ask for price |
Non-Liposomal Transfection Reagent |
abx098880-200l |
Abbexa |
200 µl |
EUR 875 |
jetOPTIMUS Transfection Reagent, 0.75mL |
PO101000025 |
Westburg |
each |
EUR 665.99 |
iMFectin 2000 DNA Transfection Reagent |
I7300-101 |
GenDepot |
1ml |
Ask for price |
iMFectin 2000 DNA Transfection Reagent |
I7300-105 |
GenDepot |
5x1ml |
Ask for price |
EnoGeneFec™ 2000 Transfection Reagent |
EGF2000-1000 |
EnoGene |
1000μl |
EUR 220 |
EnoGeneFec™ 2000 Transfection Reagent |
EGF2000-1500 |
EnoGene |
1500μl |
EUR 330 |
EnoGeneFec™ 2000 Transfection Reagent |
EGF2000-500 |
EnoGene |
500μl |
EUR 130 |
EnoGeneFec™ 2100 Transfection Reagent |
EGF2100-1000 |
EnoGene |
1000μl |
EUR 240 |
EnoGeneFec™ 2100 Transfection Reagent |
EGF2100-1500 |
EnoGene |
1500μl |
EUR 350 |
EnoGeneFec™ 2100 Transfection Reagent |
EGF2100-500 |
EnoGene |
500μl |
EUR 150 |
EnoGeneFec™ 2200 Transfection Reagent |
EGF2200-1000 |
EnoGene |
1000μl |
EUR 260 |
EnoGeneFec™ 2200 Transfection Reagent |
EGF2200-1500 |
EnoGene |
1500μl |
EUR 370 |
EnoGeneFec™ 2200 Transfection Reagent |
EGF2200-500 |
EnoGene |
500μl |
EUR 170 |
EnoGeneFec™ 2300 Transfection Reagent |
EGF2300-1000 |
EnoGene |
1000μl |
EUR 270 |
EnoGeneFec™ 2300 Transfection Reagent |
EGF2300-1500 |
EnoGene |
1500μl |
EUR 380 |
EnoGeneFec™ 2300 Transfection Reagent |
EGF2300-500 |
EnoGene |
500μl |
EUR 180 |
PureFection™ Transfection reagent |
LV750A-5 |
SBI |
5 ml |
EUR 1287 |
DNAfectin™ Plus Transfection Reagent |
E36G2500 |
EnoGene |
1.0 ml |
EUR 271.43 |
DNAfectin™ Plus Transfection Reagent |
G2500 |
ABM |
1.0 ml |
EUR 195 |
iMFectin Poly DNA Transfection Reagent |
I7200-100 |
GenDepot |
100ul |
EUR 176.4 |
iMFectin Poly DNA Transfection Reagent |
I7200-101 |
GenDepot |
1ml |
EUR 530.4 |
iMFectin Poly DNA Transfection Reagent |
I7200-105 |
GenDepot |
5x1ml |
EUR 2247.6 |
iMFectin Poly DNA Transfection Reagent |
I7200-120 |
GenDepot |
20ml |
EUR 8144.4 |
QuickShuttle-Basic Transfection Reagent |
E28X0110041 |
EnoGene |
0.8ml |
EUR 266.67 |
NutriCulture EcoTRAX Transfection Reagent |
ETRA1 |
Ecotech Biotechnology |
100 μl |
EUR 86.9 |
Description: NutriCulture EcoTRAX Transfection Reagent is formulated as a powerful transfection reagent that ensures effective and reproducible transfection with low cytotoxicity. After NutriCulture EcoTRAX Transfection Reagent and plasmid or siRNA or microRNA mixed, the NutriCulture EcoTRAX Transfection Reagent protects its cargo from degradation and facilitate efficient delivery into eukaryotic cells. The entire procedure can be completed in 30 minutes. |
NutriCulture EcoTRAX Transfection Reagent |
ETRA3 |
Ecotech Biotechnology |
3x100 μl |
EUR 218.9 |
Description: NutriCulture EcoTRAX Transfection Reagent is formulated as a powerful transfection reagent that ensures effective and reproducible transfection with low cytotoxicity. After NutriCulture EcoTRAX Transfection Reagent and plasmid or siRNA or microRNA mixed, the NutriCulture EcoTRAX Transfection Reagent protects its cargo from degradation and facilitate efficient delivery into eukaryotic cells. The entire procedure can be completed in 30 minutes. |
RNAfection, RNA transfection reagent 50 ul |
MR750A-1 |
SBI |
50 ul |
EUR 157 |
in vivo-jetRNA+ transfection reagent, 1ml |
PO101000122 |
Westburg |
each |
EUR 1503.11 |
QuickShuttle-Enhanced Transfection Reagent |
E28X0110042 |
EnoGene |
0.8ml |
EUR 266.67 |
jetMESSENGER mRNA Transfection Reagent - 0.75 ml |
PO101000005 |
Westburg |
each |
EUR 662.72 |
jetMESSENGER mRNA Transfection Reagent - 0.1 ml |
PO101000056 |
Westburg |
each |
EUR 115.54 |
QuickShuttle-Superfast Transfection Reagent |
E28X0110043 |
EnoGene |
0.8ml |
EUR 266.67 |
QuickShuttle-293(293 cell Transfection Reagent) |
E28X0110044 |
EnoGene |
0.8ml |
EUR 266.67 |
ProFectin. Transfection reagent for insect cells. |
T10 |
AB Vector LLC |
50 ul |
EUR 160 |
|
Description: Transfection |
ProFectin. Transfection reagent for insect cells. |
T20 |
AB Vector LLC |
100 ul |
EUR 295 |
|
Description: Transfection |
SuperKine™ Lipo3.0 Efficient Transfection Reagent |
BMU111-EN-05mL |
Abbkine |
0.5 mL |
EUR 49 |
SuperKine™ Lipo3.0 Efficient Transfection Reagent |
BMU111-EN-15mL |
Abbkine |
1.5 mL |
EUR 99 |
SuperKine™ Lipo3.0 Efficient Transfection Reagent |
BMU111-EN-15mL5 |
Abbkine |
1.5 mL×5 |
EUR 399 |
SuperKine™ Lipo3.0 Efficient Transfection Reagent |
BMU111-EN |
Abbkine |
0.5 mL |
Ask for price |
T-Pro Nonliposomal Transfection Reagent I (NTR I) |
JT97-N001M |
T-Pro Biotechnology |
1.0ml/vial |
EUR 224.4 |
T-Pro Nonliposomal Transfection Reagent II (NTR II) |
JT97-N002M |
T-Pro Biotechnology |
1.0ml/vial |
EUR 244.8 |
QuickShuttle-Hela(Hela cell Transfection Reagent) |
E28X0110045 |
EnoGene |
0.8ml |
EUR 266.67 |
T-Pro Nonliposomal Transfection Reagent III (NTR III) |
JT97-N006M |
T-Pro Biotechnology |
1.0ml/vial |
EUR 266.4 |
QuickShuttle-BHK-21(BHK-21 cell Transfection Reagent) |
E28X0110046 |
EnoGene |
0.8ml |
EUR 266.67 |
pPACK-SPIKE Combo Kit, includes Cat# CVD19-500A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-520A-KIT |
SBI |
1 kit |
EUR 1141 |
pPACK-SPIKE D614G Combo Kit, includes Cat# CVD19-530A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-550A-KIT |
SBI |
1 kit |
EUR 1141 |
pPACK-SPIKE N501Y Combo Kit, includes Cat# CVD19-560A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-575A-KIT |
SBI |
1 kit |
EUR 1141 |
pPACK-SPIKE Alpha Combo Kit, includes Cat# CVD19-590A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-599A-KIT |
SBI |
1 kit |
EUR 1141 |
pPACK-SPIKE Gamma Combo Kit, includes Cat# CVD19-630A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-639A-KIT |
SBI |
1 kit |
EUR 1141 |
pPACK-SPIKE Beta Combo Kit, includes Cat# CVD19-640A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-649A-KIT |
SBI |
1 kit |
EUR 1141 |
pPACK-SPIKE Delta Combo Kit, includes Cat# CVD19-650A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-659A-KIT |
SBI |
1 kit |
EUR 1141 |
pPACK-SPIKE Omicron Combo Kit, includes Cat# CVD19-660A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-669A-KIT |
SBI |
1 kit |
EUR 1162 |
pPACK-SPIKE B.1.429 Combo Kit, includes Cat# CVD19-610A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-619A-KIT |
SBI |
1 kit |
EUR 1141 |
pPACK-SPIKE B.1.525 Combo Kit, includes Cat# CVD19-620A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-629A-KIT |
SBI |
1 kit |
EUR 1141 |
pPACK-SPIKE B.1.351 RBD Mutations Combo Kit, includes Cat# CVD19-580A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-589A-KIT |
SBI |
1 kit |
EUR 1141 |
pPACK-SPIKE B.1.351 S1 Mutations Combo Kit, includes Cat# CVD19-600A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-609A-KIT |
SBI |
1 kit |
EUR 1141 |
Sapphire Baculovirus DNA and Transfection Kit |
ABP-BVD-10002 |
Allele Biotech |
1 kit |
Ask for price |
|
µ-Transfection Kit VI |
K010-0.1 |
BIONTEX |
each |
EUR 628 |
EZ-DNA Reagents |
BS8202 |
Bio Basic |
100preps |
EUR 105.67 |
|
EZ-DNA Reagents |
SK8201 |
Bio Basic |
100preps |
EUR 112.2 |
|
i-Fect Transfection Kit |
MBS555425-075mL |
MyBiosource |
0.75mL |
EUR 645 |
i-Fect Transfection Kit |
MBS555425-5x075mL |
MyBiosource |
5x0.75mL |
EUR 2755 |
293T Transfection Kit (1 mL) |
P902 |
101Bio |
1 kit |
EUR 369 |
293T Transfection Kit (0.2 mL) |
P902S |
101Bio |
- |
EUR 119 |
293T Transfection Kit (1 mL) |
P903 |
101Bio |
1 kit |
EUR 369 |
293T Transfection Kit (0.2 mL) |
P903S |
101Bio |
- |
EUR 119 |
FBS, Transfection Optimized |
F0640-050 |
GenDepot |
500ml |
EUR 902.4 |
µ-Transfection Kit VI FluoR |
K020-0.1 |
BIONTEX |
each |
EUR 720 |
Exo-Fect Exosome Transfection Kit |
EXFT10A-1 |
SBI |
10 reactions |
EUR 238 |
Exo-Fect Exosome Transfection Kit |
EXFT20A-1 |
SBI |
20 reactions |
EUR 429 |
Calcium Phosphate Cell Transfection Kit |
K1028-100 |
ApexBio |
100T |
EUR 24 |
Description: Transfection Kit, Calcium phosphate transfection method |
Calcium Phosphate Cell Transfection Kit |
K1028-200 |
ApexBio |
200T |
EUR 40 |
Description: Transfection Kit, Calcium phosphate transfection method |
Calcium Phasphate Transfection Kit (Small) |
TBS8201 |
Tribioscience |
Kit |
EUR 118 |
Calcium Phasphate Transfection Kit (Large) |
TBS8201-2 |
Tribioscience |
Kit |
EUR 328 |
Exo-Fect siRNA/miRNA Transfection Kit |
EXFT200A-1 |
SBI |
20 reactions |
EUR 412 |
MEXi-TM transfection medium 1 L |
IB2-6011-010 |
Westburg |
each |
EUR 130.8 |
CryoProtX Mix Reagents 1.5 mL |
M-MDSR-61 |
MiTeGen |
1.5 ml ml |
EUR 37 |
Description: CryoProtX Mix Reagents 1.5 mL |
Set of 10 Biolipidure Reagents |
Biolipidure-set |
AS ONE International |
10mLx10 |
EUR 1820.4 |
|
Description: Set of 10 Biolipidure Reagents, whose applications include Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
K4® Transfection System 1 x 200 µl |
T080-0.2 |
BIONTEX |
200 ul |
Ask for price |
K4® Transfection System 1 x 1 ml |
T080-1.0 |
BIONTEX |
1 ml |
EUR 260 |
K4® Transfection System 2 x 1 ml |
T080-2.0 |
BIONTEX |
1 ml |
EUR 491 |
K4® Transfection System 5 x 1 ml |
T080-5.0 |
BIONTEX |
1 m; |
EUR 1155 |
K2® Transfection System 1 x 200 µl |
T060-0.2 |
BIONTEX |
200 ul |
Ask for price |
K2® Transfection System 1 x 750 µl |
T060-0.75 |
BIONTEX |
750 ul |
EUR 231 |
K2® Transfection System 1 x 1.5 ml |
T060-1.0 |
BIONTEX |
1.5 ml |
EUR 393 |
K2® Transfection System 2 x 1.5 ml |
T060-2.0 |
BIONTEX |
1.5ml |
EUR 739 |
K2® Transfection System 5 x 1.5 ml |
T060-5.0 |
BIONTEX |
1.5 ml |
EUR 1733 |
Transfection-ready Cas9 SmartNuclease AAVS1 gRNA |
CAS520A-1 |
SBI |
10 ug |
EUR 281 |
CryoProtX Mix Eco Reagents 1.5 mL |
M-MDSR-61-ECO |
MiTeGen |
1.5 ml ml |
EUR 37 |
Description: CryoProtX Mix Eco Reagents 1.5 mL |
Transfection Collection™ : PDE4D Transient Pack |
79286 |
BPS Bioscience |
500 rxns. |
EUR 845 |
Description: The PDE4D Transient Pack is designed to provide the tools necessary for transiently transfecting and for screening inhibitors of PDE4D7 in cultured HEK293 cells._x000D_The assay is based on transfecting cells with the CRE luciferase reporter. CRE reporter contains the firefly luciferase gene under the control of cAMP response element (CRE). Elevation of intracellular cAMP activates CRE binding protein (CREB) to bind CRE and induce the expression of luciferase. Forskolin is commonly used to raise the intracellular level of cAMP in cell physiology studies. When cells transiently transfected with CRE reporter are activated by forskolin, the intracellular level of cAMP is upregulated, which induces the expression of CRE luciferase reporter._x000D_However, when cells are co-transfected with PDE4D7 expression vector and CRE reporter, the level of forskolin-induced cAMP is reduced, resulting in lower expression level of luciferase. When cells are treated with PDE4D inhibitor to inhibit PDE4D7 activity, cAMP level is restored, resulting in higher luciferase activity. The PDE4D Transient Pack contains transfection-ready CRE luciferase reporter. This reporter contains transfection-ready vectors for PDE4D7 expression and a CRE vector containing firefly luciferase as a cAMP pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control._x000D_It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells. The key to the PDE4D Cell-Based Activity Reporter Detection Valuepack is the CRE luciferase reporter vector, which is a cAMP/PKA Cell Signaling Pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized cAMP response elements (CRE) located upstream of a minimal promoter. The vector is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The pack also includes the PDE4D7 expression vector, and the adenylate cyclase activator, forskolin. Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 and HeLa cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep._x000D_Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293 or HeLa, but an alternate cell culture medium may be required for optimal cell growth |
PeliKine tool set, additional reagents |
M1980 |
Tecan Benelux |
1 unit |
EUR 169.8 |
HR based Kit in HeLa Requiring CRISPR Transfection (100ug DNA) |
DR3000-HRHeLa-CPR2 |
TopoGen |
100ug DNA |
EUR 3610.8 |
HR based Kit in HeLa Requiring CRISPR Transfection (250ug DNA) |
DR3000-HRHeLa-CPR3 |
TopoGen |
250ug DNA |
EUR 4234.8 |
HR based Kit in HeLa Requiring I-Sce1 Transfection (100ug DNA) |
DR3000-HRHeLa-Sce2 |
TopoGen |
100ug DNA |
EUR 3486 |
HR based Kit in HeLa Requiring I-Sce1 Transfection (250ug DNA) |
DR3000-HRHeLa-Sce3 |
TopoGen |
250ug DNA |
EUR 4110 |
NHEJ Based HeLa Requires I-Sce1 Transfection (100ug DNA) |
DR5001-HNHela-Sce2 |
TopoGen |
100ug DNA |
EUR 3486 |
NHEJ Based HeLa Requires I-Sce1 Transfection (250ug DNA) |
DR5001-HNHela-Sce3 |
TopoGen |
250ug DNA |
EUR 4110 |
Detection Reagent A for Dot Blot and Western Blot Kits |
WB-B006 |
PHOENIX PEPTIDE |
30 ml |
EUR 86.4 |
The potential utility of this artificial strategy is demonstrated by a late-stage modification of diethylstilbestrol. Mechanistic research revealed the a number of essential roles performed by the Ruppert-Prakash reagent.