Nippon Easy Tea Co., Ltd.

Reagents and services

Fluorine-18 labelled Ruppert-Prakash reagent ([ 18 F]Me 3 SiCF 3) for the synthesis of 18 F-trifluoromethylated compounds

By Dalton Hamster
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This text describes the primary synthesis and utility of fluorine-18 labelled Ruppert-Prakash reagent [18F]Me3SiCF3. [18F]Me3SiCF3 was synthesized from [18F]fluoroform with radiochemical yields of 85-95% and radiochemical purities of >95% inside 20 minutes. 18F-trifluoromethylated compounds had been efficiently ready by response of [18F]Me3SiCF3 with benzaldehydes, acetophenones and benzophenones.
Intact part II steroid metabolites have poor product ion mass spectra beneath collision induced dissociation (CID) situations. Due to this fact, we current herein the liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/(MS)) habits of intact part II metabolites of oxosteroids after derivatization.
Based mostly on the truth that Girard’s reagent T (GRT), as derivatization reagent, was each handy and environment friendly by way of the enhancement within the ionization effectivity and the manufacturing of diagnostic product ions associated to the steroid moiety, the latter was ideally chosen between methoxamine and hydroxylamine upon the mannequin compounds of androsterone glucuronide and androsterone sulfate.
16 totally different glucuronide and 29 sulfate conjugated metabolites of anabolic androgenic steroids (AAS), out there both as pure reference supplies, or synthesized/extracted from administration research, had been derivatized with GRT and their product ion spectra are offered. Product ion spectra embrace in all instances excessive variety of product ions that in some instances are attribute for sure constructions of the steroid spine.
Extra particularly, preliminary outcomes have proven main variations in fragmentation sample for 17α/17β-isomers of the sulfate-conjugates, however restricted differentiation for 17α/17β-isomers of glucuronide-conjugates and for 3α/3β- and 5α/5β-stereoisomers of each sulfate and glucuronide conjugates. Additional to the suggestion of the present work, utility on mesterolone administration research confirmed -according to the WADA TD2015IDCR- the presence of seven intact part II metabolites, one glucuronide and 6 sulfates with use of LC-ESI-MS/(MS).

Dedication of nitrofuran metabolites in honey utilizing a brand new derivatization reagent, magnetic solid-phase extraction and LC-MS/MS

On this research, 5-nitro-2-furaldehyde (5-NFA) was proposed as a brand new derivatizing agent for nitrofuran metabolites. It reacts with nitrofuran metabolites producing the guardian nitrofurans (furazolidone, furaltadone, nitrofurantoin, and nitrofurazone). Magnetic hypercrosslinked polystyrene (HCP/Fe3O4) was first used for magnetic stable part extraction (MSPE) clean-up earlier than the dedication of nitrofuran metabolite derivatives in honey by way of LC-MS/MS.
Primary parameters affecting the derivatization and MSPE effectivity had been investigated intimately and the optimum situations had been discovered. The tactic was validated utilizing honey spiked with the 4 metabolites at 1, 2 and 200 μg kg-1. Recoveries of >85% had been achieved for the all analytes. The matrix calibration curve was fitted with the correlation coefficient (R2) > 0.99 within the vary of 1-200 μg kg-1.
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Precision values expressed as relative commonplace deviation (RSD) had been <12% and <15% for intra-day and inter-day precision, respectively. The boundaries of detection (LODs) for the nitrofuran metabolites had been of 0.1-0.Three μg kg-1 and the bounds of quantitation (LOQs) had been of 0.3-1.Zero μg kg-1. The proposed LC-MS/MS technique was utilized to the evaluation of actual honey samples.

Peptide Bond Formation of Amino Acids by Transient Masking with Silylating Reagents

A one-pot peptide bond-forming response has been developed utilizing unprotected amino acids and peptides. Two totally different silylating reagents, HSi[OCH(CF3)2]3 and MTBSTFA, are instrumental for the profitable implementation of this strategy, getting used for the activation and transient masking of unprotected amino acids and peptides at C-termini and N-termini, respectively.
Moreover, CsF and imidazole are used as catalysts, activating HSi[OCH(CF3)2]3 and in addition accelerating chemoselective silylation. This technique is flexible because it tolerates facet chains that bear a variety of useful teams, whereas offering as much as >99% yields of corresponding peptides with none racemization or polymerization.

Hydrothermal Crystallization of Bismuth Oxychlorides (BiOCl) Utilizing Completely different Form Management Reagents

Bismuth oxychloride photocatalysts had been obtained utilizing solvothermal synthesis and totally different components (CTAB-cetyltrimethylammonium bromide, CTAC-cetyltrimethylammonium chloride, PVP-polyvinylpyrrolidone, SDS-sodium dodecylsulphate, U-urea and TU-thiourea). The impact of the beforehand talked about compounds was analyzed making use of structural (main crystallite measurement, crystal part composition, and so forth.), morphological (particle geometry), optical (band hole vitality) parameters, floor associated properties (floor atoms’ oxidation states), and the resulted photocatalytic exercise.
A powerful dependency was discovered between the floor pressure of the synthesis options and the general morpho-structural parameters. The primary discovering was that the traits of the semiconductors could be tuned by modifying the floor pressure of the synthesis combination. It was noticed after the photocatalytic degradation, that the white semiconductor turned to gray. Moreover, we tried to clarify the grey shade of BiOCl catalysts after the photocatalytic decompositions by Raman and XPS research.

Difluorocarbene-Mediated Cascade Cyclization: The Multifunctional Function of Ruppert-Prakash Reagent

A difluorocarbene-mediated cascade cyclization response for fast entry to gem-difluorinated 3-coumaranone derivatives was developed. The difluorocarbene acts as a bipolar CF2 constructing block, which permits a homologation cyclization course of by way of sequentially reacting with the phenolate and the ester group on the identical substrate.

LP4K Transfection Reagent
LP4K 1.0 ml / vial
EUR 175
Description: Lipid based transfection reagent for large plasmid and multiple plasmid transfection in both adhesive and suspenstion cell types.

GeneGlide? siRNA Transfection Reagent
M1081-1000 each
EUR 534

GeneGlide? siRNA Transfection Reagent
M1081-300 each
EUR 318

GeneGlide? siRNA Transfection Reagent
M1081-500 each
EUR 364.8

Transfection Reagent (1 mL)
P901 1 mL
EUR 369

Transfection Reagent (0.1 mL)
P901S 0.1 mL
EUR 119

Convoy? Transfection Reagent
1110-1ml each
EUR 409.2

Transfection Reagent (in vivo)
MBS635395-012mL 0.12mL
EUR 1160

Transfection Reagent (in vivo)
MBS635395-5x012mL 5x0.12mL
EUR 5075

NanoFect Transfection Reagent
NF100 1 ml
EUR 189.63

HighGene transfection reagent
RM09014 1000μl
EUR 324

Transfection Reagent (in vitro)
MBS635268-1mL 1mL
EUR 755

Transfection Reagent (in vitro)
MBS635268-5x1mL 5x1mL
EUR 3240

p-Fect, Transfection reagent
MBS555424-5x75Reactions 5x75Reactions
EUR 1780

p-Fect, Transfection reagent
MBS555424-75Reactions 75Reactions
EUR 430

Liposomal Transfection Reagent
abx298031-100g 100 µg Ask for price

Liposomal Transfection Reagent
abx298031-20g 20 µg
EUR 575

Liposomal Transfection Reagent
abx298031-50g 50 µg
EUR 875

Susfectin Transfection Reagent
MBS4160451-1mL 1mL
EUR 270

Susfectin Transfection Reagent
MBS4160451-5x1mL 5x1mL
EUR 960

Convoy™ Transfection Reagent
1110 1ml
EUR 251.55

n-Blast Transfection Reagent
MBS555427-075mL 0.75mL
EUR 460

n-Blast Transfection Reagent
MBS555427-5x075mL 5x0.75mL
EUR 1910

Transfectamineâ„¢ 5000 Transfection Reagent
60020-05mL 0.5 mL
EUR 222
Description: Transfectamine™ 5000 Transfection Reagent is a powerful and versatile transfection reagent for the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells.

Transfectamineâ„¢ 5000 Transfection Reagent
60021-1mL 1 mL
EUR 334
Description: Transfectamine™ 5000 Transfection Reagent is a powerful and versatile transfection reagent for the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells.

Transfectamineâ„¢ 5000 Transfection Reagent
60022-5mL 5 mL
EUR 846
Description: Transfectamine™ 5000 Transfection Reagent is a powerful and versatile transfection reagent for the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells.

RNAifectin Transfection Reagent
MBS8580098-1mL 1mL
EUR 305

RNAifectin Transfection Reagent
MBS8580098-5x1mL 5x1mL
EUR 1235

ExFect2000 Transfection Reagent
T202-01 0.5 ml
EUR 272.4

ExFect2000 Transfection Reagent
T202-02 1 ml
EUR 379.2

ExFect2000 Transfection Reagent
T202-03 5 ml
EUR 1262.4

Lentifectin Transfection Reagent
MBS8580099-1mL 1mL
EUR 290

Lentifectin Transfection Reagent
MBS8580099-5x1mL 5x1mL
EUR 1160

UltraTRAXTM Transfection Reagent
MB508-0100 100 µL Ask for price

UltraTRAXTM Transfection Reagent
MB508-1000 1000 µL Ask for price

DNAfectin 2100 Transfection Reagent
MBS8580096-1mg 1mg
EUR 280

DNAfectin 2100 Transfection Reagent
MBS8580096-5x1mg 5x1mg
EUR 1115

Transfectamineâ„¢ mRNA Transfection Reagent
60030-500ul 500 ul
EUR 203
Description: Transfectamineâ„¢ mRNA Transfection Reagent is a powerful and versatile transfection reagent designed to introduce a higher amount of mRNA into eukaryotic cells, or more specifically, into animal cells.

Transfectamineâ„¢ mRNA Transfection Reagent
60031-5ml 5 ml
EUR 989
Description: Transfectamineâ„¢ mRNA Transfection Reagent is a powerful and versatile transfection reagent designed to introduce a higher amount of mRNA into eukaryotic cells, or more specifically, into animal cells.

iMFectin DNA Transfection Reagent
I7100-100 100ul
EUR 176.4

iMFectin DNA Transfection Reagent
I7100-101 1ml
EUR 310

iMFectin DNA Transfection Reagent
I7100-105 5x1ml
EUR 1408

iMFectin DNA Transfection Reagent
I7100-120 20ml
EUR 8144.4

Susfectin™ Transfection Reagent
G4000 1.0 ml
EUR 195

EnoGeneFec 2000 Transfection Reagent
MBS8579497-05mL 0.5mL
EUR 220

EnoGeneFec 2000 Transfection Reagent
MBS8579497-15mL 1.5mL
EUR 410

EnoGeneFec 2000 Transfection Reagent
MBS8579497-1mL 1mL
EUR 300

EnoGeneFec 2000 Transfection Reagent
MBS8579497-5x15mL 5x1.5mL
EUR 1685

EnoGeneFec 2100 Transfection Reagent
MBS8579500-05mL 0.5mL
EUR 240

EnoGeneFec 2100 Transfection Reagent
MBS8579500-15mL 1.5mL
EUR 430

EnoGeneFec 2100 Transfection Reagent
MBS8579500-1mL 1mL
EUR 315

EnoGeneFec 2100 Transfection Reagent
MBS8579500-5x15mL 5x1.5mL
EUR 1780

EnoGeneFec 2200 Transfection Reagent
MBS8579503-05mL 0.5mL
EUR 255

EnoGeneFec 2200 Transfection Reagent
MBS8579503-15mL 1.5mL
EUR 450

EnoGeneFec 2200 Transfection Reagent
MBS8579503-1mL 1mL
EUR 335

EnoGeneFec 2200 Transfection Reagent
MBS8579503-5x15mL 5x1.5mL
EUR 1870

EnoGeneFec 2300 Transfection Reagent
MBS8579506-05mL 0.5mL
EUR 260

EnoGeneFec 2300 Transfection Reagent
MBS8579506-15mL 1.5mL
EUR 460

EnoGeneFec 2300 Transfection Reagent
MBS8579506-1mL 1mL
EUR 345

EnoGeneFec 2300 Transfection Reagent
MBS8579506-5x15mL 5x1.5mL
EUR 1920

Lipomaster 2000 Transfection Reagent
TL201-00-50ul 50 μl
EUR 1.09

Lipomaster 2000 Transfection Reagent
TL201-01-15ml 1.5 ml
EUR 305.75

Lipomaster 2000 Transfection Reagent
TL201-02-415ml 4 × 1.5 ml
EUR 1035.5

Lipomaster 3000 Transfection Reagent
TL301-00-50ul 50 μl
EUR 1.09

Lipomaster 3000 Transfection Reagent
TL301-01-15ml 1.5 ml
EUR 414.2

Lipomaster 3000 Transfection Reagent
TL301-02-315ml 3 ×1.5 ml
EUR 912.33

RNAifectin™ Transfection Reagent
E36G073 1.0 ml (100-500 transfections)
EUR 228.57

Lentifectin (TM) Transfection Reagent
DNAF003 1ml
EUR 334.57

Lentifectin™ Transfection Reagent
E36G074 1.0 ml
EUR 214.29

DNAfectin Plus Transfection Reagent
MBS4160450-1mL 1mL
EUR 270

DNAfectin Plus Transfection Reagent
MBS4160450-5x1mL 5x1mL
EUR 960

DNAfectin Plus Transfection Reagent
MBS8580097-1mL 1mL
EUR 345

DNAfectin Plus Transfection Reagent
MBS8580097-5x1mL 5x1mL
EUR 1395

MAXgene® GMP Transfection Reagent
26406-1 1L Ask for price

DNAfectin™ 2100 Transfection Reagent
E36G2100 1.0 mg/1.0 ml
EUR 200

baculoFECTIN II Transfection Reagent 150 ul
GWB-300101 150 ul Ask for price

i-Fect si RNA Transfection Reagent
MBS555708-5x075mL 5x0.75mL
EUR 2475

Sapphire Insect Transfection Reagent
ABP-BVD-10003 75ul, 25 transfections Ask for price

Non-Liposomal Transfection Reagent
abx098880-100l 100 µl
EUR 575

Non-Liposomal Transfection Reagent
abx098880-1ml 1 ml Ask for price

Non-Liposomal Transfection Reagent
abx098880-200l 200 µl
EUR 875

baculoFECTIN II transfection reagent
GWB-300106 1 ml Ask for price

iMFectin 2000 DNA Transfection Reagent
I7300-101 1ml Ask for price

iMFectin 2000 DNA Transfection Reagent
I7300-105 5x1ml Ask for price

Transporter 5® Transfection Reagent
26008-1A 1ml
EUR 74.52

Transporter 5® Transfection Reagent
26008-5 5ml
EUR 345.6

Transporter 5® Transfection Reagent
26008-50 50ml
EUR 1391.04

EnoGeneFec™ 2000 Transfection Reagent
EGF2000-1000 1000μl
EUR 220

EnoGeneFec™ 2000 Transfection Reagent
EGF2000-1500 1500μl
EUR 330

EnoGeneFec™ 2000 Transfection Reagent
EGF2000-500 500μl
EUR 130

EnoGeneFec™ 2100 Transfection Reagent
EGF2100-1000 1000μl
EUR 240

EnoGeneFec™ 2100 Transfection Reagent
EGF2100-1500 1500μl
EUR 350

EnoGeneFec™ 2100 Transfection Reagent
EGF2100-500 500μl
EUR 150

EnoGeneFec™ 2200 Transfection Reagent
EGF2200-1000 1000μl
EUR 260

EnoGeneFec™ 2200 Transfection Reagent
EGF2200-1500 1500μl
EUR 370

EnoGeneFec™ 2200 Transfection Reagent
EGF2200-500 500μl
EUR 170

EnoGeneFec™ 2300 Transfection Reagent
EGF2300-1000 1000μl
EUR 270

EnoGeneFec™ 2300 Transfection Reagent
EGF2300-1500 1500μl
EUR 380

EnoGeneFec™ 2300 Transfection Reagent
EGF2300-500 500μl
EUR 180

T-Pro P-Fect Transfection Reagent
JT97-N005M 1.0ml/vial
EUR 266.4

iMFectin Poly DNA Transfection Reagent
I7200-100 100ul
EUR 176.4

iMFectin Poly DNA Transfection Reagent
I7200-101 1ml
EUR 310

iMFectin Poly DNA Transfection Reagent
I7200-105 5x1ml
EUR 1386

iMFectin Poly DNA Transfection Reagent
I7200-120 20ml
EUR 8144.4

DNAfectin™ Plus Transfection Reagent
G2500 1.0 ml
EUR 195

DNAfectin™ Plus Transfection Reagent
E36G2500 1.0 ml
EUR 271.43

NutriCulture EcoTRAX Transfection Reagent
ETRA1 100 μl
EUR 86.9
Description: NutriCulture EcoTRAX Transfection Reagent is formulated as a powerful transfection reagent that ensures effective and reproducible transfection with low cytotoxicity. After NutriCulture EcoTRAX Transfection Reagent and plasmid or siRNA or microRNA mixed, the NutriCulture EcoTRAX Transfection Reagent protects its cargo from degradation and facilitate efficient delivery into eukaryotic cells. The entire procedure can be completed in 30 minutes.

NutriCulture EcoTRAX Transfection Reagent
ETRA3 3x100 μl
EUR 218.9
Description: NutriCulture EcoTRAX Transfection Reagent is formulated as a powerful transfection reagent that ensures effective and reproducible transfection with low cytotoxicity. After NutriCulture EcoTRAX Transfection Reagent and plasmid or siRNA or microRNA mixed, the NutriCulture EcoTRAX Transfection Reagent protects its cargo from degradation and facilitate efficient delivery into eukaryotic cells. The entire procedure can be completed in 30 minutes.

QuickShuttle-Basic Transfection Reagent
E28X0110041 0.8ml
EUR 266.67

MAXgene® GMP Transfection Reagent, Powder
26435-1 1g Ask for price

Hieff TransTM Liposomal Transfection Reagent
40802ES02 0.5 mL
EUR 212

Hieff TransTM Liposomal Transfection Reagent
40802ES03 1 mL
EUR 342

Hieff Trans® PEI Transfection Reagent-GMP
40821ES10 10 mL
EUR 2155

Hieff Trans® PEI Transfection Reagent-GMP
40821ES60 100 mL
EUR 8625

QuickShuttle-Enhanced Transfection Reagent
E28X0110042 0.8ml
EUR 266.67

QuickShuttle-Superfast Transfection Reagent
E28X0110043 0.8ml
EUR 266.67

QuickShuttle-293(293 cell Transfection Reagent)
E28X0110044 0.8ml
EUR 266.67

ProFectin. Transfection reagent for insect cells.
T10 50 ul
EUR 160
Description: Transfection

ProFectin. Transfection reagent for insect cells.
T20 100 ul
EUR 295
Description: Transfection

Hieff TransTM PEI Transfection Reagent Hieff TransTM
40820ES04 1.5 mL
EUR 225

Hieff TransTM PEI Transfection Reagent Hieff TransTM
40820ES10 10 mL
EUR 865

Hieff TransTM PEI Transfection Reagent Hieff TransTM
40820ES60 100 mL
EUR 3445

SuperKine™ Lipo3.0 Efficient Transfection Reagent
BMU111-EN 0.5 mL Ask for price

SuperKine™ Lipo3.0 Efficient Transfection Reagent
BMU111-EN-05mL 0.5 mL
EUR 49

SuperKine™ Lipo3.0 Efficient Transfection Reagent
BMU111-EN-15mL 1.5 mL
EUR 99

SuperKine™ Lipo3.0 Efficient Transfection Reagent
BMU111-EN-15mL5 1.5 mL×5
EUR 399

T-Pro Nonliposomal Transfection Reagent I (NTR I)
JT97-N001M 1.0ml/vial
EUR 110

T-Pro Nonliposomal Transfection Reagent II (NTR II)
JT97-N002M 1.0ml/vial
EUR 130

QuickShuttle-Hela(Hela cell Transfection Reagent)
E28X0110045 0.8ml
EUR 266.67

Hieff TransTM in vitro siRNA/miRNA Transfection Reagent
40806ES01 0.1 mL
EUR 62

Hieff TransTM in vitro siRNA/miRNA Transfection Reagent
40806ES02 0.5 mL
EUR 362

Hieff TransTM in vitro siRNA/miRNA Transfection Reagent
40806ES03 1 mL
EUR 482

T-Pro Nonliposomal Transfection Reagent III (NTR III)
JT97-N006M 1.0ml/vial
EUR 150

QuickShuttle-BHK-21(BHK-21 cell Transfection Reagent)
E28X0110046 0.8ml
EUR 266.67

Sapphire Baculovirus DNA and Transfection Kit
ABP-BVD-10002 1 kit Ask for price

EVfect Transfection Kit
JOT-EV-T1 each
EUR 239.53

µ-Transfection Kit VI
K010-0.1 each
EUR 439.6

i-Fect Transfection Kit
MBS555425-075mL 0.75mL
EUR 645

i-Fect Transfection Kit
MBS555425-5x075mL 5x0.75mL
EUR 2755

OET Transfection Medium
500312 50 ml
EUR 48.51

293T Transfection Kit (1 mL)
P902 1 kit
EUR 369

293T Transfection Kit (0.2 mL)
P902S -
EUR 119

293T Transfection Kit (1 mL)
P903 1 kit
EUR 369

293T Transfection Kit (0.2 mL)
P903S -
EUR 119

FBS, Transfection Optimized
F0640-050 500ml
EUR 902.4

µ-Transfection Kit VI FluoR
K020-0.1 each
EUR 504

K2® Transfection System
T060-0.2 200 ul Ask for price

K4® Transfection System
T080-0.2 200 ul Ask for price

EZ-DNA Reagents
SK8201 100preps
EUR 112.2

Calcium Phosphate Transfection Kit
GR103070 100 preps
EUR 219

Transfection Grade (<0.05 EU/ug)
GC0046 100ug
EUR 48.3

Transfection Grade (<0.05 EU/ug)
GC0047 200ug
EUR 69.3

Transfection Grade (<0.05 EU/ug)
GC0048 500ug
EUR 111.3

Transfection Grade (<0.05 EU/ug)
GC0049 1 mg
EUR 132.3

Transfection Grade (<0.05 EU/ug)
GC0050 2 mg
EUR 167.3

Transfection Grade (<0.05 EU/ug)
GC0051 5mg
EUR 272.3

Transfection Grade (<0.05 EU/ug)
GC0052 10 mg (High-copy)
EUR 349.3

Transfection Grade (<0.05 EU/ug)
GC0053 10 mg (Low-copy)
EUR 559.3

Transfection Grade (<0.05 EU/ug)
GC0054 > 10 mg Ask for price

Reagents Module Assy.
U10071 1 Each Ask for price

Ecl Detection Reagents
RPN3004 EACH
EUR 552.9

Calcium Phosphate Cell Transfection Kit
K1028-100 100T
EUR 40
Description: Cell Biology|Cell Transfection

Calcium Phosphate Cell Transfection Kit
K1028-200 200T
EUR 56
Description: Cell Biology|Cell Transfection

Protoplast Solution for Transfection
GR103052 10 mL
EUR 119

Calcium Phasphate Transfection Kit (Small)
TBS8201 Kit
EUR 118

Calcium Phasphate Transfection Kit (Large)
TBS8201-2 Kit
EUR 328

Reagents Refrigiration Assy.
U10072 1 Each Ask for price

RANGE 13 - Ventilated cabinet for reagents and toxic products - 250 L
MF4V each
EUR 2377.76

RANGE 13 - Cabinet for reagents and toxic products (without fan) - 250 L
MF4SV. each
EUR 2158.24

K2® Transfection System 1 x 750 µl
T060-0.75 750 ul
EUR 161.7

K2® Transfection System 1 x 1.5 ml
T060-1.0 1.5 ml
EUR 275.1

K2® Transfection System 2 x 1.5 ml
T060-2.0 1.5ml
EUR 517.3

K2® Transfection System 5 x 1.5 ml
T060-5.0 1.5 ml
EUR 1212.4

K4® Transfection System 1 x 1 ml
T080-1.0 1 ml
EUR 182

K4® Transfection System 2 x 1 ml
T080-2.0 1 ml
EUR 343.7

K4® Transfection System 5 x 1 ml
T080-5.0 1 m;
EUR 808.5

Ecl Detection Reagents - EACH
RPN2105 EACH
EUR 815.4

Phosphate-buffered Sucrose for Transfection
GR103050 1 L
EUR 119

Plant Electroporation Buffer for Transfection
GR103051 1 L
EUR 119

Transfection Collection™ : PDE4D Transient Pack
79286 500 rxns.
EUR 845
Description: The PDE4D Transient Pack is designed to provide the tools necessary for transiently transfecting and for screening inhibitors of PDE4D7 in cultured HEK293 cells._x000D_The assay is based on transfecting cells with the CRE luciferase reporter. CRE reporter contains the firefly luciferase gene under the control of cAMP response element (CRE). Elevation of intracellular cAMP activates CRE binding protein (CREB) to bind CRE and induce the expression of luciferase. Forskolin is commonly used to raise the intracellular level of cAMP in cell physiology studies. When cells transiently transfected with CRE reporter are activated by forskolin, the intracellular level of cAMP is upregulated, which induces the expression of CRE luciferase reporter._x000D_However, when cells are co-transfected with PDE4D7 expression vector and CRE reporter, the level of forskolin-induced cAMP is reduced, resulting in lower expression level of luciferase. When cells are treated with PDE4D inhibitor to inhibit PDE4D7 activity, cAMP level is restored, resulting in higher luciferase activity. The PDE4D Transient Pack contains transfection-ready CRE luciferase reporter. This reporter contains transfection-ready vectors for PDE4D7 expression and a CRE vector containing firefly luciferase as a cAMP pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control._x000D_It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells. The key to the PDE4D Cell-Based Activity Reporter Detection Valuepack is the CRE luciferase reporter vector, which is a cAMP/PKA Cell Signaling Pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized cAMP response elements (CRE) located upstream of a minimal promoter. The vector is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The pack also includes the PDE4D7 expression vector, and the adenylate cyclase activator, forskolin. Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 and HeLa cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep._x000D_Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293 or HeLa, but an alternate cell culture medium may be required for optimal cell growth

HR based Kit in HeLa Requiring CRISPR Transfection (100ug DNA)
DR3000-HRHeLa-CPR2 100ug DNA
EUR 3610.8

HR based Kit in HeLa Requiring CRISPR Transfection (250ug DNA)
DR3000-HRHeLa-CPR3 250ug DNA
EUR 4234.8

CryoProtX Mix Reagents 1.5 mL
M-MDSR-61 1.5 ml ml
EUR 37
Description: CryoProtX Mix Reagents 1.5 mL

HR based Kit in HeLa Requiring I-Sce1 Transfection (100ug DNA)
DR3000-HRHeLa-Sce2 100ug DNA
EUR 3486

HR based Kit in HeLa Requiring I-Sce1 Transfection (250ug DNA)
DR3000-HRHeLa-Sce3 250ug DNA
EUR 4110

NHEJ Based HeLa Requires I-Sce1 Transfection (100ug DNA)
DR5001-HNHela-Sce2 100ug DNA
EUR 3486

NHEJ Based HeLa Requires I-Sce1 Transfection (250ug DNA)
DR5001-HNHela-Sce3 250ug DNA
EUR 4110

Set of 10 Biolipidure Reagents
Biolipidure-set 10mLx10
EUR 1820.4
Description: Set of 10 Biolipidure Reagents, whose applications include Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

REAGENTS HIGH RANGE AMMONIA - PK25
HI73325 PK25
EUR 55.35

High Range Ammonia Reagents - EACH
HI9373301 EACH
EUR 130.95

CryoProtX Mix Eco Reagents 1.5 mL
M-MDSR-61-ECO 1.5 ml ml
EUR 37
Description: CryoProtX Mix Eco Reagents 1.5 mL

PeliKine tool set, additional reagents
M1980 1 unit
EUR 169.8

100 x DEAE-dextran Stock Solution for Transfection
GR103049 10 x 10 mL
EUR 119

Alkphos Direct Labelling Reagents - EACH
RPN3680 EACH
EUR 915.3

HR based Kit in HeLa Requiring CRISPR Transfection (50ug DNA)
DR3000-HRHeLa-CPR 50ug DNA
EUR 3298.8

HR based Kit in MOUSE 3T3 Cells Requiring I-Sce1 Transfection (100ug DNA)
DR3000-HR3T3-Sce2 100ug DNA
EUR 3486
The potential utility of this artificial strategy is demonstrated by a late-stage modification of diethylstilbestrol. Mechanistic research revealed the a number of essential roles performed by the Ruppert-Prakash reagent.

Dalton

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Monoclonal Antibodies
Polyclonal Antibodies