Fluorine-18 labelled Ruppert-Prakash reagent ([ 18 F]Me 3 SiCF 3) for the synthesis of 18 F-trifluoromethylated compounds

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This text describes the primary synthesis and utility of fluorine-18 labelled Ruppert-Prakash reagent [18F]Me3SiCF3. [18F]Me3SiCF3 was synthesized from [18F]fluoroform with radiochemical yields of 85-95% and radiochemical purities of >95% inside 20 minutes. 18F-trifluoromethylated compounds had been efficiently ready by response of [18F]Me3SiCF3 with benzaldehydes, acetophenones and benzophenones.
Intact part II steroid metabolites have poor product ion mass spectra beneath collision induced dissociation (CID) situations. Due to this fact, we current herein the liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/(MS)) habits of intact part II metabolites of oxosteroids after derivatization.
Based mostly on the truth that Girard’s reagent T (GRT), as derivatization reagent, was each handy and environment friendly by way of the enhancement within the ionization effectivity and the manufacturing of diagnostic product ions associated to the steroid moiety, the latter was ideally chosen between methoxamine and hydroxylamine upon the mannequin compounds of androsterone glucuronide and androsterone sulfate.
16 totally different glucuronide and 29 sulfate conjugated metabolites of anabolic androgenic steroids (AAS), out there both as pure reference supplies, or synthesized/extracted from administration research, had been derivatized with GRT and their product ion spectra are offered. Product ion spectra embrace in all instances excessive variety of product ions that in some instances are attribute for sure constructions of the steroid spine.
Extra particularly, preliminary outcomes have proven main variations in fragmentation sample for 17α/17β-isomers of the sulfate-conjugates, however restricted differentiation for 17α/17β-isomers of glucuronide-conjugates and for 3α/3β- and 5α/5β-stereoisomers of each sulfate and glucuronide conjugates. Additional to the suggestion of the present work, utility on mesterolone administration research confirmed -according to the WADA TD2015IDCR- the presence of seven intact part II metabolites, one glucuronide and 6 sulfates with use of LC-ESI-MS/(MS).

Dedication of nitrofuran metabolites in honey utilizing a brand new derivatization reagent, magnetic solid-phase extraction and LC-MS/MS

On this research, 5-nitro-2-furaldehyde (5-NFA) was proposed as a brand new derivatizing agent for nitrofuran metabolites. It reacts with nitrofuran metabolites producing the guardian nitrofurans (furazolidone, furaltadone, nitrofurantoin, and nitrofurazone). Magnetic hypercrosslinked polystyrene (HCP/Fe3O4) was first used for magnetic stable part extraction (MSPE) clean-up earlier than the dedication of nitrofuran metabolite derivatives in honey by way of LC-MS/MS.
Primary parameters affecting the derivatization and MSPE effectivity had been investigated intimately and the optimum situations had been discovered. The tactic was validated utilizing honey spiked with the 4 metabolites at 1, 2 and 200 μg kg-1. Recoveries of >85% had been achieved for the all analytes. The matrix calibration curve was fitted with the correlation coefficient (R2) > 0.99 within the vary of 1-200 μg kg-1.
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Precision values expressed as relative commonplace deviation (RSD) had been <12% and <15% for intra-day and inter-day precision, respectively. The boundaries of detection (LODs) for the nitrofuran metabolites had been of 0.1-0.Three μg kg-1 and the bounds of quantitation (LOQs) had been of 0.3-1.Zero μg kg-1. The proposed LC-MS/MS technique was utilized to the evaluation of actual honey samples.

Peptide Bond Formation of Amino Acids by Transient Masking with Silylating Reagents

A one-pot peptide bond-forming response has been developed utilizing unprotected amino acids and peptides. Two totally different silylating reagents, HSi[OCH(CF3)2]3 and MTBSTFA, are instrumental for the profitable implementation of this strategy, getting used for the activation and transient masking of unprotected amino acids and peptides at C-termini and N-termini, respectively.
Moreover, CsF and imidazole are used as catalysts, activating HSi[OCH(CF3)2]3 and in addition accelerating chemoselective silylation. This technique is flexible because it tolerates facet chains that bear a variety of useful teams, whereas offering as much as >99% yields of corresponding peptides with none racemization or polymerization.

Hydrothermal Crystallization of Bismuth Oxychlorides (BiOCl) Utilizing Completely different Form Management Reagents

Bismuth oxychloride photocatalysts had been obtained utilizing solvothermal synthesis and totally different components (CTAB-cetyltrimethylammonium bromide, CTAC-cetyltrimethylammonium chloride, PVP-polyvinylpyrrolidone, SDS-sodium dodecylsulphate, U-urea and TU-thiourea). The impact of the beforehand talked about compounds was analyzed making use of structural (main crystallite measurement, crystal part composition, and so forth.), morphological (particle geometry), optical (band hole vitality) parameters, floor associated properties (floor atoms’ oxidation states), and the resulted photocatalytic exercise.
A powerful dependency was discovered between the floor pressure of the synthesis options and the general morpho-structural parameters. The primary discovering was that the traits of the semiconductors could be tuned by modifying the floor pressure of the synthesis combination. It was noticed after the photocatalytic degradation, that the white semiconductor turned to gray. Moreover, we tried to clarify the grey shade of BiOCl catalysts after the photocatalytic decompositions by Raman and XPS research.

Difluorocarbene-Mediated Cascade Cyclization: The Multifunctional Function of Ruppert-Prakash Reagent

A difluorocarbene-mediated cascade cyclization response for fast entry to gem-difluorinated 3-coumaranone derivatives was developed. The difluorocarbene acts as a bipolar CF2 constructing block, which permits a homologation cyclization course of by way of sequentially reacting with the phenolate and the ester group on the identical substrate.

GeneGlide? siRNA Transfection Reagent

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GeneGlide? siRNA Transfection Reagent

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GeneGlide? siRNA Transfection Reagent

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GENIUS DNA Transfection Reagent - 1.0 ml

WB7-1010 each
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Transfection Reagent (1 mL)

P901 1 mL
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Transfection Reagent (0.1 mL)

P901S 0.1 mL
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Convoy? Transfection Reagent

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EUR 409.2

Transfection Reagent (in vivo)

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Transfection Reagent (in vivo)

MBS635395-5x012mL 5x0.12mL
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NanoFect Transfection Reagent

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EUR 189.63

HighGene transfection reagent

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EUR 324

Transfection Reagent (in vitro)

MBS635268-1mL 1mL
EUR 755

Transfection Reagent (in vitro)

MBS635268-5x1mL 5x1mL
EUR 3240

p-Fect, Transfection reagent

MBS555424-5x75Reactions 5x75Reactions
EUR 1780

p-Fect, Transfection reagent

MBS555424-75Reactions 75Reactions
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Liposomal Transfection Reagent

abx298031-100g 100 µg Ask for price

Liposomal Transfection Reagent

abx298031-20g 20 µg
EUR 575

Liposomal Transfection Reagent

abx298031-50g 50 µg
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Susfectin Transfection Reagent

MBS4160451-1mL 1mL
EUR 270

Susfectin Transfection Reagent

MBS4160451-5x1mL 5x1mL
EUR 960

GENIUS DNA Transfection Reagent-5 x WB 7-1010 (5x1.0ml)

WB7-1050 each
EUR 970.1

Convoy™ Transfection Reagent

1110 1ml
EUR 251.55

n-Blast Transfection Reagent

MBS555427-075mL 0.75mL
EUR 460

n-Blast Transfection Reagent

MBS555427-5x075mL 5x0.75mL
EUR 1910

Transfectamineâ„¢ 5000 Transfection Reagent

60020-05mL 0.5 mL
EUR 222
Description: Transfectamine™ 5000 Transfection Reagent is a powerful and versatile transfection reagent for the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells.

Transfectamineâ„¢ 5000 Transfection Reagent

60021-1mL 1 mL
EUR 334
Description: Transfectamine™ 5000 Transfection Reagent is a powerful and versatile transfection reagent for the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells.

Transfectamineâ„¢ 5000 Transfection Reagent

60022-5mL 5 mL
EUR 846
Description: Transfectamine™ 5000 Transfection Reagent is a powerful and versatile transfection reagent for the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells.

ExFect2000 Transfection Reagent

T202-01 0.5 ml
EUR 272.4

ExFect2000 Transfection Reagent

T202-02 1 ml
EUR 379.2

ExFect2000 Transfection Reagent

T202-03 5 ml
EUR 1262.4

RNAifectin Transfection Reagent

MBS8580098-1mL 1mL
EUR 305

RNAifectin Transfection Reagent

MBS8580098-5x1mL 5x1mL
EUR 1235

PureFection Transfection Reagent

LV750A-1 1 ml
EUR 303

Lentifectin Transfection Reagent

MBS8580099-1mL 1mL
EUR 290

Lentifectin Transfection Reagent

MBS8580099-5x1mL 5x1mL
EUR 1160

DNAfectin 2100 Transfection Reagent

MBS8580096-1mg 1mg
EUR 280

DNAfectin 2100 Transfection Reagent

MBS8580096-5x1mg 5x1mg
EUR 1115

Transfectamineâ„¢ mRNA Transfection Reagent

60030-500ul 500 ul
EUR 203
Description: Transfectamineâ„¢ mRNA Transfection Reagent is a powerful and versatile transfection reagent designed to introduce a higher amount of mRNA into eukaryotic cells, or more specifically, into animal cells.

Transfectamineâ„¢ mRNA Transfection Reagent

60031-5ml 5 ml
EUR 989
Description: Transfectamineâ„¢ mRNA Transfection Reagent is a powerful and versatile transfection reagent designed to introduce a higher amount of mRNA into eukaryotic cells, or more specifically, into animal cells.

Susfectin™ Transfection Reagent

G4000 1.0 ml
EUR 195

iMFectin DNA Transfection Reagent

I7100-100 100ul
EUR 176.4

iMFectin DNA Transfection Reagent

I7100-101 1ml
EUR 530.4

iMFectin DNA Transfection Reagent

I7100-105 5x1ml
EUR 2247.6

iMFectin DNA Transfection Reagent

I7100-120 20ml
EUR 8144.4

EnoGeneFec 2000 Transfection Reagent

MBS8579497-05mL 0.5mL
EUR 220

EnoGeneFec 2000 Transfection Reagent

MBS8579497-15mL 1.5mL
EUR 410

EnoGeneFec 2000 Transfection Reagent

MBS8579497-1mL 1mL
EUR 300

EnoGeneFec 2000 Transfection Reagent

MBS8579497-5x15mL 5x1.5mL
EUR 1685

EnoGeneFec 2100 Transfection Reagent

MBS8579500-05mL 0.5mL
EUR 240

EnoGeneFec 2100 Transfection Reagent

MBS8579500-15mL 1.5mL
EUR 430

EnoGeneFec 2100 Transfection Reagent

MBS8579500-1mL 1mL
EUR 315

EnoGeneFec 2100 Transfection Reagent

MBS8579500-5x15mL 5x1.5mL
EUR 1780

EnoGeneFec 2200 Transfection Reagent

MBS8579503-05mL 0.5mL
EUR 255

EnoGeneFec 2200 Transfection Reagent

MBS8579503-15mL 1.5mL
EUR 450

EnoGeneFec 2200 Transfection Reagent

MBS8579503-1mL 1mL
EUR 335

EnoGeneFec 2200 Transfection Reagent

MBS8579503-5x15mL 5x1.5mL
EUR 1870

EnoGeneFec 2300 Transfection Reagent

MBS8579506-05mL 0.5mL
EUR 260

EnoGeneFec 2300 Transfection Reagent

MBS8579506-15mL 1.5mL
EUR 460

EnoGeneFec 2300 Transfection Reagent

MBS8579506-1mL 1mL
EUR 345

EnoGeneFec 2300 Transfection Reagent

MBS8579506-5x15mL 5x1.5mL
EUR 1920

Lipomaster 2000 Transfection Reagent

TL201-00-50ul 50 μl
EUR 1.09

Lipomaster 2000 Transfection Reagent

TL201-01-15ml 1.5 ml
EUR 305.75

Lipomaster 2000 Transfection Reagent

TL201-02-415ml 4 × 1.5 ml
EUR 1035.5

Lipomaster 3000 Transfection Reagent

TL301-00-50ul 50 μl
EUR 1.09

Lipomaster 3000 Transfection Reagent

TL301-01-15ml 1.5 ml
EUR 414.2

Lipomaster 3000 Transfection Reagent

TL301-02-315ml 3 ×1.5 ml
EUR 912.33

RNAifectin™ Transfection Reagent

E36G073 1.0 ml (100-500 transfections)
EUR 228.57

Lentifectin (TM) Transfection Reagent

DNAF003 1ml
EUR 334.57

Lentifectin™ Transfection Reagent

E36G074 1.0 ml
EUR 214.29

DNAfectin Plus Transfection Reagent

MBS8580097-1mL 1mL
EUR 345

DNAfectin Plus Transfection Reagent

MBS8580097-5x1mL 5x1mL
EUR 1395

DNAfectin Plus Transfection Reagent

MBS4160450-1mL 1mL
EUR 270

DNAfectin Plus Transfection Reagent

MBS4160450-5x1mL 5x1mL
EUR 960

jetOPTIMUS Transfection Reagent, 1.5mL

PO101000006 each
EUR 1132.51

jetOPTIMUS Transfection Reagent, 0.1mL

PO101000051 each
EUR 98.1

MAXgene® GMP Transfection Reagent

26406-1 1L Ask for price

DNAfectin™ 2100 Transfection Reagent

E36G2100 1.0 mg/1.0 ml
EUR 200

baculoFECTIN II Transfection Reagent 150 ul

GWB-300101 150 ul Ask for price

i-Fect si RNA Transfection Reagent

MBS555708-5x075mL 5x0.75mL
EUR 2475

Sapphire Insect Transfection Reagent

ABP-BVD-10003 75ul, 25 transfections Ask for price

baculoFECTIN II transfection reagent

GWB-300106 1 ml Ask for price

Non-Liposomal Transfection Reagent

abx098880-100l 100 µl
EUR 575

Non-Liposomal Transfection Reagent

abx098880-1ml 1 ml Ask for price

Non-Liposomal Transfection Reagent

abx098880-200l 200 µl
EUR 875

jetOPTIMUS Transfection Reagent, 0.75mL

PO101000025 each
EUR 665.99

iMFectin 2000 DNA Transfection Reagent

I7300-101 1ml Ask for price

iMFectin 2000 DNA Transfection Reagent

I7300-105 5x1ml Ask for price

Transporter 5® Transfection Reagent

26008-1A 1ml
EUR 65

Transporter 5® Transfection Reagent

26008-5 5ml
EUR 304

Transporter 5® Transfection Reagent

26008-50 50ml
EUR 1226

EnoGeneFec™ 2000 Transfection Reagent

EGF2000-1000 1000μl
EUR 220

EnoGeneFec™ 2000 Transfection Reagent

EGF2000-1500 1500μl
EUR 330

EnoGeneFec™ 2000 Transfection Reagent

EGF2000-500 500μl
EUR 130

EnoGeneFec™ 2100 Transfection Reagent

EGF2100-1000 1000μl
EUR 240

EnoGeneFec™ 2100 Transfection Reagent

EGF2100-1500 1500μl
EUR 350

EnoGeneFec™ 2100 Transfection Reagent

EGF2100-500 500μl
EUR 150

EnoGeneFec™ 2200 Transfection Reagent

EGF2200-1000 1000μl
EUR 260

EnoGeneFec™ 2200 Transfection Reagent

EGF2200-1500 1500μl
EUR 370

EnoGeneFec™ 2200 Transfection Reagent

EGF2200-500 500μl
EUR 170

EnoGeneFec™ 2300 Transfection Reagent

EGF2300-1000 1000μl
EUR 270

EnoGeneFec™ 2300 Transfection Reagent

EGF2300-1500 1500μl
EUR 380

EnoGeneFec™ 2300 Transfection Reagent

EGF2300-500 500μl
EUR 180

T-Pro P-Fect Transfection Reagent

JT97-N005M 1.0ml/vial
EUR 266.4

PureFection™ Transfection reagent

LV750A-5 5 ml
EUR 1287

DNAfectin™ Plus Transfection Reagent

E36G2500 1.0 ml
EUR 271.43

DNAfectin™ Plus Transfection Reagent

G2500 1.0 ml
EUR 195

iMFectin Poly DNA Transfection Reagent

I7200-100 100ul
EUR 176.4

iMFectin Poly DNA Transfection Reagent

I7200-101 1ml
EUR 530.4

iMFectin Poly DNA Transfection Reagent

I7200-105 5x1ml
EUR 2247.6

iMFectin Poly DNA Transfection Reagent

I7200-120 20ml
EUR 8144.4

QuickShuttle-Basic Transfection Reagent

E28X0110041 0.8ml
EUR 266.67

RNAfection, RNA transfection reagent 50 ul

MR750A-1 50 ul
EUR 157

in vivo-jetRNA+ transfection reagent, 1ml

PO101000122 each
EUR 1503.11

MAXgene® GMP Transfection Reagent, Powder

26435-1 1g Ask for price

QuickShuttle-Enhanced Transfection Reagent

E28X0110042 0.8ml
EUR 266.67

jetMESSENGER mRNA Transfection Reagent - 0.75 ml

PO101000005 each
EUR 662.72

jetMESSENGER mRNA Transfection Reagent - 0.1 ml

PO101000056 each
EUR 115.54

QuickShuttle-Superfast Transfection Reagent

E28X0110043 0.8ml
EUR 266.67

QuickShuttle-293(293 cell Transfection Reagent)

E28X0110044 0.8ml
EUR 266.67

ProFectin. Transfection reagent for insect cells.

T10 50 ul
EUR 160
Description: Transfection

ProFectin. Transfection reagent for insect cells.

T20 100 ul
EUR 295
Description: Transfection

SuperKine™ Lipo3.0 Efficient Transfection Reagent

BMU111-EN-05mL 0.5 mL
EUR 49

SuperKine™ Lipo3.0 Efficient Transfection Reagent

BMU111-EN-15mL 1.5 mL
EUR 99

SuperKine™ Lipo3.0 Efficient Transfection Reagent

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EUR 399

SuperKine™ Lipo3.0 Efficient Transfection Reagent

BMU111-EN 0.5 mL Ask for price

T-Pro Nonliposomal Transfection Reagent I (NTR I)

JT97-N001M 1.0ml/vial
EUR 224.4

T-Pro Nonliposomal Transfection Reagent II (NTR II)

JT97-N002M 1.0ml/vial
EUR 244.8

QuickShuttle-Hela(Hela cell Transfection Reagent)

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EUR 266.67

T-Pro Nonliposomal Transfection Reagent III (NTR III)

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EUR 266.4

QuickShuttle-BHK-21(BHK-21 cell Transfection Reagent)

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EUR 266.67

pPACK-SPIKE Combo Kit, includes Cat# CVD19-500A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-520A-KIT 1 kit
EUR 1141

pPACK-SPIKE D614G Combo Kit, includes Cat# CVD19-530A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-550A-KIT 1 kit
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pPACK-SPIKE N501Y Combo Kit, includes Cat# CVD19-560A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-575A-KIT 1 kit
EUR 1141

pPACK-SPIKE Alpha Combo Kit, includes Cat# CVD19-590A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-599A-KIT 1 kit
EUR 1141

pPACK-SPIKE Gamma Combo Kit, includes Cat# CVD19-630A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-639A-KIT 1 kit
EUR 1141

pPACK-SPIKE Beta Combo Kit, includes Cat# CVD19-640A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-649A-KIT 1 kit
EUR 1141

pPACK-SPIKE Delta Combo Kit, includes Cat# CVD19-650A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-659A-KIT 1 kit
EUR 1141

pPACK-SPIKE Omicron Combo Kit, includes Cat# CVD19-660A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-669A-KIT 1 kit
EUR 1162

pPACK-SPIKE B.1.429 Combo Kit, includes Cat# CVD19-610A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-619A-KIT 1 kit
EUR 1141

pPACK-SPIKE B.1.525 Combo Kit, includes Cat# CVD19-620A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-629A-KIT 1 kit
EUR 1141

pPACK-SPIKE B.1.351 RBD Mutations Combo Kit, includes Cat# CVD19-580A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-589A-KIT 1 kit
EUR 1141

pPACK-SPIKE B.1.351 S1 Mutations Combo Kit, includes Cat# CVD19-600A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-609A-KIT 1 kit
EUR 1141

Sapphire Baculovirus DNA and Transfection Kit

ABP-BVD-10002 1 kit Ask for price

µ-Transfection Kit VI

K010-0.1 each
EUR 628

EZ-DNA Reagents

BS8202 100preps
EUR 105.67

EZ-DNA Reagents

SK8201 100preps
EUR 112.2

i-Fect Transfection Kit

MBS555425-075mL 0.75mL
EUR 645

i-Fect Transfection Kit

MBS555425-5x075mL 5x0.75mL
EUR 2755

OET Transfection Medium

500312 50 ml
EUR 68.2

Ecl Detection Reagents

RPN3004 EACH
EUR 552.9

293T Transfection Kit (1 mL)

P902 1 kit
EUR 369

293T Transfection Kit (0.2 mL)

P902S -
EUR 119

293T Transfection Kit (1 mL)

P903 1 kit
EUR 369

293T Transfection Kit (0.2 mL)

P903S -
EUR 119

FBS, Transfection Optimized

F0640-050 500ml
EUR 902.4

µ-Transfection Kit VI FluoR

K020-0.1 each
EUR 720

Calcium Phosphate Transfection Kit

GR103070 100 preps
EUR 219

Exo-Fect Exosome Transfection Kit

EXFT10A-1 10 reactions
EUR 238

Exo-Fect Exosome Transfection Kit

EXFT20A-1 20 reactions
EUR 429

Ecl Detection Reagents - EACH

RPN2105 EACH
EUR 815.4

Calcium Phosphate Cell Transfection Kit

K1028-100 100T
EUR 24
Description: Transfection Kit, Calcium phosphate transfection method

Calcium Phosphate Cell Transfection Kit

K1028-200 200T
EUR 40
Description: Transfection Kit, Calcium phosphate transfection method

Protoplast Solution for Transfection

GR103052 10 mL
EUR 119

Calcium Phasphate Transfection Kit (Small)

TBS8201 Kit
EUR 118

Calcium Phasphate Transfection Kit (Large)

TBS8201-2 Kit
EUR 328

Exo-Fect siRNA/miRNA Transfection Kit

EXFT200A-1 20 reactions
EUR 412

CryoProtX Mix Reagents 1.5 mL

M-MDSR-61 1.5 ml ml
EUR 37
Description: CryoProtX Mix Reagents 1.5 mL

MEXi-TM transfection medium 1 L

IB2-6011-010 each
EUR 130.8

Set of 10 Biolipidure Reagents

Biolipidure-set 10mLx10
EUR 1820.4
Description: Set of 10 Biolipidure Reagents, whose applications include Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

K4® Transfection System 1 x 200 µl

T080-0.2 200 ul Ask for price

K4® Transfection System 1 x 1 ml

T080-1.0 1 ml
EUR 260

K4® Transfection System 2 x 1 ml

T080-2.0 1 ml
EUR 491

K4® Transfection System 5 x 1 ml

T080-5.0 1 m;
EUR 1155

K2® Transfection System 1 x 200 µl

T060-0.2 200 ul Ask for price

K2® Transfection System 1 x 750 µl

T060-0.75 750 ul
EUR 231

K2® Transfection System 1 x 1.5 ml

T060-1.0 1.5 ml
EUR 393

K2® Transfection System 2 x 1.5 ml

T060-2.0 1.5ml
EUR 739

K2® Transfection System 5 x 1.5 ml

T060-5.0 1.5 ml
EUR 1733

REAGENTS HIGH RANGE AMMONIA - PK25

HI73325 PK25
EUR 55.35

High Range Ammonia Reagents - EACH

HI9373301 EACH
EUR 130.95

CryoProtX Mix Eco Reagents 1.5 mL

M-MDSR-61-ECO 1.5 ml ml
EUR 37
Description: CryoProtX Mix Eco Reagents 1.5 mL

Transfection-ready Cas9 SmartNuclease AAVS1 gRNA

CAS520A-1 10 ug
EUR 281

Phosphate-buffered Sucrose for Transfection

GR103050 1 L
EUR 119

Plant Electroporation Buffer for Transfection

GR103051 1 L
EUR 119

PeliKine tool set, additional reagents

M1980 1 unit
EUR 169.8

Transfection Collection™ : PDE4D Transient Pack

79286 500 rxns.
EUR 845
Description: The PDE4D Transient Pack is designed to provide the tools necessary for transiently transfecting and for screening inhibitors of PDE4D7 in cultured HEK293 cells._x000D_The assay is based on transfecting cells with the CRE luciferase reporter. CRE reporter contains the firefly luciferase gene under the control of cAMP response element (CRE). Elevation of intracellular cAMP activates CRE binding protein (CREB) to bind CRE and induce the expression of luciferase. Forskolin is commonly used to raise the intracellular level of cAMP in cell physiology studies. When cells transiently transfected with CRE reporter are activated by forskolin, the intracellular level of cAMP is upregulated, which induces the expression of CRE luciferase reporter._x000D_However, when cells are co-transfected with PDE4D7 expression vector and CRE reporter, the level of forskolin-induced cAMP is reduced, resulting in lower expression level of luciferase. When cells are treated with PDE4D inhibitor to inhibit PDE4D7 activity, cAMP level is restored, resulting in higher luciferase activity. The PDE4D Transient Pack contains transfection-ready CRE luciferase reporter. This reporter contains transfection-ready vectors for PDE4D7 expression and a CRE vector containing firefly luciferase as a cAMP pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control._x000D_It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells. The key to the PDE4D Cell-Based Activity Reporter Detection Valuepack is the CRE luciferase reporter vector, which is a cAMP/PKA Cell Signaling Pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized cAMP response elements (CRE) located upstream of a minimal promoter. The vector is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The pack also includes the PDE4D7 expression vector, and the adenylate cyclase activator, forskolin. Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 and HeLa cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep._x000D_Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293 or HeLa, but an alternate cell culture medium may be required for optimal cell growth

Alkphos Direct Labelling Reagents - EACH

RPN3680 EACH
EUR 915.3

HR based Kit in HeLa Requiring CRISPR Transfection (100ug DNA)

DR3000-HRHeLa-CPR2 100ug DNA
EUR 3610.8

HR based Kit in HeLa Requiring CRISPR Transfection (250ug DNA)

DR3000-HRHeLa-CPR3 250ug DNA
EUR 4234.8

Detection Reagent A for Dot Blot and Western Blot Kits

WB-B006 30 ml
EUR 86.4

Detection Reagent B for Dot Blot and Western Blot Kits

WB-B007 30 ml
EUR 86.4

HR based Kit in HeLa Requiring I-Sce1 Transfection (100ug DNA)

DR3000-HRHeLa-Sce2 100ug DNA
EUR 3486

HR based Kit in HeLa Requiring I-Sce1 Transfection (250ug DNA)

DR3000-HRHeLa-Sce3 250ug DNA
EUR 4110

NHEJ Based HeLa Requires I-Sce1 Transfection (100ug DNA)

DR5001-HNHela-Sce2 100ug DNA
EUR 3486

NHEJ Based HeLa Requires I-Sce1 Transfection (250ug DNA)

DR5001-HNHela-Sce3 250ug DNA
EUR 4110

Free Chlorine Reagents 0-5mg/L - EACH

WAT1028 EACH
EUR 49.95
The potential utility of this artificial strategy is demonstrated by a late-stage modification of diethylstilbestrol. Mechanistic research revealed the a number of essential roles performed by the Ruppert-Prakash reagent.

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