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Reagents and services

Fluorine-18 labelled Ruppert-Prakash reagent ([ 18 F]Me 3 SiCF 3) for the synthesis of 18 F-trifluoromethylated compounds

By Dalton Hamster
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This text describes the primary synthesis and utility of fluorine-18 labelled Ruppert-Prakash reagent [18F]Me3SiCF3. [18F]Me3SiCF3 was synthesized from [18F]fluoroform with radiochemical yields of 85-95% and radiochemical purities of >95% inside 20 minutes. 18F-trifluoromethylated compounds had been efficiently ready by response of [18F]Me3SiCF3 with benzaldehydes, acetophenones and benzophenones.
Intact part II steroid metabolites have poor product ion mass spectra beneath collision induced dissociation (CID) situations. Due to this fact, we current herein the liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/(MS)) habits of intact part II metabolites of oxosteroids after derivatization.
Based mostly on the truth that Girard’s reagent T (GRT), as derivatization reagent, was each handy and environment friendly by way of the enhancement within the ionization effectivity and the manufacturing of diagnostic product ions associated to the steroid moiety, the latter was ideally chosen between methoxamine and hydroxylamine upon the mannequin compounds of androsterone glucuronide and androsterone sulfate.
16 totally different glucuronide and 29 sulfate conjugated metabolites of anabolic androgenic steroids (AAS), out there both as pure reference supplies, or synthesized/extracted from administration research, had been derivatized with GRT and their product ion spectra are offered. Product ion spectra embrace in all instances excessive variety of product ions that in some instances are attribute for sure constructions of the steroid spine.
Extra particularly, preliminary outcomes have proven main variations in fragmentation sample for 17α/17β-isomers of the sulfate-conjugates, however restricted differentiation for 17α/17β-isomers of glucuronide-conjugates and for 3α/3β- and 5α/5β-stereoisomers of each sulfate and glucuronide conjugates. Additional to the suggestion of the present work, utility on mesterolone administration research confirmed -according to the WADA TD2015IDCR- the presence of seven intact part II metabolites, one glucuronide and 6 sulfates with use of LC-ESI-MS/(MS).

Dedication of nitrofuran metabolites in honey utilizing a brand new derivatization reagent, magnetic solid-phase extraction and LC-MS/MS

On this research, 5-nitro-2-furaldehyde (5-NFA) was proposed as a brand new derivatizing agent for nitrofuran metabolites. It reacts with nitrofuran metabolites producing the guardian nitrofurans (furazolidone, furaltadone, nitrofurantoin, and nitrofurazone). Magnetic hypercrosslinked polystyrene (HCP/Fe3O4) was first used for magnetic stable part extraction (MSPE) clean-up earlier than the dedication of nitrofuran metabolite derivatives in honey by way of LC-MS/MS.
Primary parameters affecting the derivatization and MSPE effectivity had been investigated intimately and the optimum situations had been discovered. The tactic was validated utilizing honey spiked with the 4 metabolites at 1, 2 and 200 μg kg-1. Recoveries of >85% had been achieved for the all analytes. The matrix calibration curve was fitted with the correlation coefficient (R2) > 0.99 within the vary of 1-200 μg kg-1.
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Precision values expressed as relative commonplace deviation (RSD) had been <12% and <15% for intra-day and inter-day precision, respectively. The boundaries of detection (LODs) for the nitrofuran metabolites had been of 0.1-0.Three μg kg-1 and the bounds of quantitation (LOQs) had been of 0.3-1.Zero μg kg-1. The proposed LC-MS/MS technique was utilized to the evaluation of actual honey samples.

Peptide Bond Formation of Amino Acids by Transient Masking with Silylating Reagents

A one-pot peptide bond-forming response has been developed utilizing unprotected amino acids and peptides. Two totally different silylating reagents, HSi[OCH(CF3)2]3 and MTBSTFA, are instrumental for the profitable implementation of this strategy, getting used for the activation and transient masking of unprotected amino acids and peptides at C-termini and N-termini, respectively.
Moreover, CsF and imidazole are used as catalysts, activating HSi[OCH(CF3)2]3 and in addition accelerating chemoselective silylation. This technique is flexible because it tolerates facet chains that bear a variety of useful teams, whereas offering as much as >99% yields of corresponding peptides with none racemization or polymerization.

Hydrothermal Crystallization of Bismuth Oxychlorides (BiOCl) Utilizing Completely different Form Management Reagents

Bismuth oxychloride photocatalysts had been obtained utilizing solvothermal synthesis and totally different components (CTAB-cetyltrimethylammonium bromide, CTAC-cetyltrimethylammonium chloride, PVP-polyvinylpyrrolidone, SDS-sodium dodecylsulphate, U-urea and TU-thiourea). The impact of the beforehand talked about compounds was analyzed making use of structural (main crystallite measurement, crystal part composition, and so forth.), morphological (particle geometry), optical (band hole vitality) parameters, floor associated properties (floor atoms’ oxidation states), and the resulted photocatalytic exercise.
A powerful dependency was discovered between the floor pressure of the synthesis options and the general morpho-structural parameters. The primary discovering was that the traits of the semiconductors could be tuned by modifying the floor pressure of the synthesis combination. It was noticed after the photocatalytic degradation, that the white semiconductor turned to gray. Moreover, we tried to clarify the grey shade of BiOCl catalysts after the photocatalytic decompositions by Raman and XPS research.

Difluorocarbene-Mediated Cascade Cyclization: The Multifunctional Function of Ruppert-Prakash Reagent

A difluorocarbene-mediated cascade cyclization response for fast entry to gem-difluorinated 3-coumaranone derivatives was developed. The difluorocarbene acts as a bipolar CF2 constructing block, which permits a homologation cyclization course of by way of sequentially reacting with the phenolate and the ester group on the identical substrate.

GeneGlide? siRNA Transfection Reagent
M1081-1000 each
EUR 534

GeneGlide? siRNA Transfection Reagent
M1081-300 each
EUR 318

GeneGlide? siRNA Transfection Reagent
M1081-500 each
EUR 364.8

HighGene transfection reagent
RM09014 1000μl
EUR 324

NanoFect Transfection Reagent
NF100 1 ml
EUR 189.63

Convoy™ Transfection Reagent
1110 1ml
EUR 251.55

Transfectamineâ„¢ 5000 Transfection Reagent
60020-05mL 0.5 mL
EUR 222
Description: Transfectamine™ 5000 Transfection Reagent is a powerful and versatile transfection reagent for the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells.

Transfectamineâ„¢ 5000 Transfection Reagent
60021-1mL 1 mL
EUR 334
Description: Transfectamine™ 5000 Transfection Reagent is a powerful and versatile transfection reagent for the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells.

Transfectamineâ„¢ 5000 Transfection Reagent
60022-5mL 5 mL
EUR 846
Description: Transfectamine™ 5000 Transfection Reagent is a powerful and versatile transfection reagent for the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells.

ExFect2000 Transfection Reagent
T202-01 0.5 ml
EUR 272.4

ExFect2000 Transfection Reagent
T202-02 1 ml
EUR 379.2

ExFect2000 Transfection Reagent
T202-03 5 ml
EUR 1262.4

PureFection Transfection Reagent
LV750A-1 1 ml
EUR 303

Transfectamineâ„¢ mRNA Transfection Reagent
60030-500ul 500 ul
EUR 203
Description: Transfectamineâ„¢ mRNA Transfection Reagent is a powerful and versatile transfection reagent designed to introduce a higher amount of mRNA into eukaryotic cells, or more specifically, into animal cells.

Transfectamineâ„¢ mRNA Transfection Reagent
60031-5ml 5 ml
EUR 989
Description: Transfectamineâ„¢ mRNA Transfection Reagent is a powerful and versatile transfection reagent designed to introduce a higher amount of mRNA into eukaryotic cells, or more specifically, into animal cells.

iMFectin DNA Transfection Reagent
I7100-100 100ul
EUR 176.4

iMFectin DNA Transfection Reagent
I7100-101 1ml
EUR 530.4

iMFectin DNA Transfection Reagent
I7100-105 5x1ml
EUR 2247.6

iMFectin DNA Transfection Reagent
I7100-120 20ml
EUR 8144.4

RNAifectin™ Transfection Reagent
E36G073 1.0 ml (100-500 transfections)
EUR 228.57

Transfectamine™ mRNA Transfection Reagent
60030 500 ul
EUR 203

MAXgene® GMP Transfection Reagent
26406-1 1L Ask for price

Lentifectin (TM) Transfection Reagent
DNAF003 1ml
EUR 334.57

Lentifectin™ Transfection Reagent
E36G074 1.0 ml
EUR 214.29

DNAfectin™ 2100 Transfection Reagent
E36G2100 1.0 mg/1.0 ml
EUR 200

iMFectin 2000 DNA Transfection Reagent
I7300-101 1ml Ask for price

iMFectin 2000 DNA Transfection Reagent
I7300-105 5x1ml Ask for price

Transporter 5® Transfection Reagent
26008-1A 1ml
EUR 65

Transporter 5® Transfection Reagent
26008-5 5ml
EUR 304

Transporter 5® Transfection Reagent
26008-50 50ml
EUR 1226

Sapphire Insect Transfection Reagent
ABP-BVD-10003 75ul, 25 transfections Ask for price

EnoGeneFec™ 2000 Transfection Reagent
EGF2000-1000 1000μl
EUR 220

EnoGeneFec™ 2000 Transfection Reagent
EGF2000-1500 1500μl
EUR 330

EnoGeneFec™ 2000 Transfection Reagent
EGF2000-500 500μl
EUR 130

EnoGeneFec™ 2100 Transfection Reagent
EGF2100-1000 1000μl
EUR 240

EnoGeneFec™ 2100 Transfection Reagent
EGF2100-1500 1500μl
EUR 350

EnoGeneFec™ 2100 Transfection Reagent
EGF2100-500 500μl
EUR 150

EnoGeneFec™ 2200 Transfection Reagent
EGF2200-1000 1000μl
EUR 260

EnoGeneFec™ 2200 Transfection Reagent
EGF2200-1500 1500μl
EUR 370

EnoGeneFec™ 2200 Transfection Reagent
EGF2200-500 500μl
EUR 170

EnoGeneFec™ 2300 Transfection Reagent
EGF2300-1000 1000μl
EUR 270

EnoGeneFec™ 2300 Transfection Reagent
EGF2300-1500 1500μl
EUR 380

EnoGeneFec™ 2300 Transfection Reagent
EGF2300-500 500μl
EUR 180

T-Pro P-Fect Transfection Reagent
JT97-N005M 1.0ml/vial
EUR 266.4

PureFection™ Transfection reagent
LV750A-5 5 ml
EUR 1287

iMFectin Poly DNA Transfection Reagent
I7200-100 100ul
EUR 176.4

iMFectin Poly DNA Transfection Reagent
I7200-101 1ml
EUR 530.4

iMFectin Poly DNA Transfection Reagent
I7200-105 5x1ml
EUR 2247.6

iMFectin Poly DNA Transfection Reagent
I7200-120 20ml
EUR 8144.4

DNAfectin™ Plus Transfection Reagent
E36G2500 1.0 ml
EUR 271.43

RNAfection, RNA transfection reagent 50 ul
MR750A-1 50 ul
EUR 157

QuickShuttle-Basic Transfection Reagent
E28X0110041 0.8ml
EUR 266.67

MAXgene® GMP Transfection Reagent, Powder
26435-1 1g Ask for price

QuickShuttle-Enhanced Transfection Reagent
E28X0110042 0.8ml
EUR 266.67

QuickShuttle-Superfast Transfection Reagent
E28X0110043 0.8ml
EUR 266.67

QuickShuttle-293(293 cell Transfection Reagent)
E28X0110044 0.8ml
EUR 266.67

T-Pro Nonliposomal Transfection Reagent I (NTR I)
JT97-N001M 1.0ml/vial
EUR 224.4

T-Pro Nonliposomal Transfection Reagent II (NTR II)
JT97-N002M 1.0ml/vial
EUR 244.8

QuickShuttle-Hela(Hela cell Transfection Reagent)
E28X0110045 0.8ml
EUR 266.67

T-Pro Nonliposomal Transfection Reagent III (NTR III)
JT97-N006M 1.0ml/vial
EUR 266.4

QuickShuttle-BHK-21(BHK-21 cell Transfection Reagent)
E28X0110046 0.8ml
EUR 266.67

pPACK-SPIKE Combo Kit, includes Cat# CVD19-500A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-520A-KIT 1 kit
EUR 1141

pPACK-SPIKE D614G Combo Kit, includes Cat# CVD19-530A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-550A-KIT 1 kit
EUR 1141

pPACK-SPIKE N501Y Combo Kit, includes Cat# CVD19-560A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-575A-KIT 1 kit
EUR 1141

pPACK-SPIKE Alpha Combo Kit, includes Cat# CVD19-590A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-599A-KIT 1 kit
EUR 1141

pPACK-SPIKE Gamma Combo Kit, includes Cat# CVD19-630A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-639A-KIT 1 kit
EUR 1141

pPACK-SPIKE Beta Combo Kit, includes Cat# CVD19-640A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-649A-KIT 1 kit
EUR 1141

pPACK-SPIKE Delta Combo Kit, includes Cat# CVD19-650A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-659A-KIT 1 kit
EUR 1141

pPACK-SPIKE Omicron Combo Kit, includes Cat# CVD19-660A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-669A-KIT 1 kit
EUR 1162

pPACK-SPIKE B.1.429 Combo Kit, includes Cat# CVD19-610A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-619A-KIT 1 kit
EUR 1141

pPACK-SPIKE B.1.525 Combo Kit, includes Cat# CVD19-620A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-629A-KIT 1 kit
EUR 1141

Sapphire Baculovirus DNA and Transfection Kit
ABP-BVD-10002 1 kit Ask for price

pPACK-SPIKE B.1.351 RBD Mutations Combo Kit, includes Cat# CVD19-580A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-589A-KIT 1 kit
EUR 1141

pPACK-SPIKE B.1.351 S1 Mutations Combo Kit, includes Cat# CVD19-600A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-609A-KIT 1 kit
EUR 1141

OET Transfection Medium
500312 50 ml
EUR 68.2

293T Transfection Kit (1 mL)
P902 - Ask for price

293T Transfection Kit (0.2 mL)
P902S - Ask for price

293T Transfection Kit (1 mL)
P903 - Ask for price

293T Transfection Kit (0.2 mL)
P903S - Ask for price

FBS, Transfection Optimized
F0640-050 500ml
EUR 902.4

Calcium Phosphate Transfection Kit
GR103070 100 preps
EUR 219

Exo-Fect Exosome Transfection Kit
EXFT10A-1 10 reactions
EUR 238

Exo-Fect Exosome Transfection Kit
EXFT20A-1 20 reactions
EUR 429

Protoplast Solution for Transfection
GR103052 10 mL
EUR 119

Calcium Phosphate Cell Transfection Kit
K1028-100 100T
EUR 30
Description: Transfection Kit, Calcium phosphate transfection method

Calcium Phosphate Cell Transfection Kit
K1028-200 200T
EUR 50
Description: Transfection Kit, Calcium phosphate transfection method

Exo-Fect siRNA/miRNA Transfection Kit
EXFT200A-1 20 reactions
EUR 412

Transfection-ready Cas9 SmartNuclease AAVS1 gRNA
CAS520A-1 10 ug
EUR 281

Transfection Collection™ : PDE4D Transient Pack
79286 500 rxns.
EUR 845
Description: The PDE4D Transient Pack is designed to provide the tools necessary for transiently transfecting and for screening inhibitors of PDE4D7 in cultured HEK293 cells._x000D_The assay is based on transfecting cells with the CRE luciferase reporter. CRE reporter contains the firefly luciferase gene under the control of cAMP response element (CRE). Elevation of intracellular cAMP activates CRE binding protein (CREB) to bind CRE and induce the expression of luciferase. Forskolin is commonly used to raise the intracellular level of cAMP in cell physiology studies. When cells transiently transfected with CRE reporter are activated by forskolin, the intracellular level of cAMP is upregulated, which induces the expression of CRE luciferase reporter._x000D_However, when cells are co-transfected with PDE4D7 expression vector and CRE reporter, the level of forskolin-induced cAMP is reduced, resulting in lower expression level of luciferase. When cells are treated with PDE4D inhibitor to inhibit PDE4D7 activity, cAMP level is restored, resulting in higher luciferase activity. The PDE4D Transient Pack contains transfection-ready CRE luciferase reporter. This reporter contains transfection-ready vectors for PDE4D7 expression and a CRE vector containing firefly luciferase as a cAMP pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control._x000D_It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells. The key to the PDE4D Cell-Based Activity Reporter Detection Valuepack is the CRE luciferase reporter vector, which is a cAMP/PKA Cell Signaling Pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized cAMP response elements (CRE) located upstream of a minimal promoter. The vector is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The pack also includes the PDE4D7 expression vector, and the adenylate cyclase activator, forskolin. Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 and HeLa cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep._x000D_Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293 or HeLa, but an alternate cell culture medium may be required for optimal cell growth

Phosphate-buffered Sucrose for Transfection
GR103050 1 L
EUR 119

Plant Electroporation Buffer for Transfection
GR103051 1 L
EUR 119

HR based Kit in HeLa Requiring CRISPR Transfection (100ug DNA)
DR3000-HRHeLa-CPR2 100ug DNA
EUR 3610.8

HR based Kit in HeLa Requiring CRISPR Transfection (250ug DNA)
DR3000-HRHeLa-CPR3 250ug DNA
EUR 4234.8

NHEJ Based HeLa Requires I-Sce1 Transfection (100ug DNA)
DR5001-HNHela-Sce2 100ug DNA
EUR 3486

NHEJ Based HeLa Requires I-Sce1 Transfection (250ug DNA)
DR5001-HNHela-Sce3 250ug DNA
EUR 4110

HR based Kit in HeLa Requiring I-Sce1 Transfection (100ug DNA)
DR3000-HRHeLa-Sce2 100ug DNA
EUR 3486

HR based Kit in HeLa Requiring I-Sce1 Transfection (250ug DNA)
DR3000-HRHeLa-Sce3 250ug DNA
EUR 4110

100 x DEAE-dextran Stock Solution for Transfection
GR103049 10 x 10 mL
EUR 119

Polyethylenimine, Linear, MW 25000, Transfection Grade (PEI 25K„¢)
23966-1 1g
EUR 1093
Description: 9002-98-6

Polyethylenimine, Linear, MW 25000, Transfection Grade (PEI 25K„¢)
23966-100 100mg
EUR 209
Description: 9002-98-6

HR based Kit in HeLa Requiring CRISPR Transfection (50ug DNA)
DR3000-HRHeLa-CPR 50ug DNA
EUR 3298.8

NHEJ Based HeLa Requires I-Sce1 Transfection (50ug DNA)
DR5001-HNHela-Sce 50ug DNA
EUR 3174

Transfection-ready Cas9 SmartNuclease mRNA (Eukaryotic version)
CAS500A-1 20 ug
EUR 387

"N" SARS-CoV-2 Nucleocapsid mRNA (10 µg, Transfection-ready)
CVD19-420A-1 10 µg
EUR 328

"N" SARS-CoV-2 Nucleocapsid mRNA (20 µg, Transfection-ready)
CVD19-425A-1 20 µg
EUR 468

HR in human Neuroblastoma Cells req. I-Sce1 Transfection (100ug DNA)
DR3000-HRSH-Sce2 100ug DNA
EUR 3735.6

HR in human Neuroblastoma Cells req. I-Sce1 Transfection (250ug DNA)
DR3000-HRSH-Sce3 250ug DNA
EUR 4359.6

NHEJ Based Mouse 3T3 cells Requires I-Sce1 Transfection (100ug DNA)
DR5000-HN3T3-Sce2 100ug DNA
EUR 3486

NHEJ Based Mouse 3T3 cells Requires I-Sce1 Transfection (250ug DNA)
DR5000-HN3T3-Sce3 250ug DNA
EUR 4110

"S" SARS-CoV-2 Spike Omicron mRNA (10 µg, Transfection-ready)
CVD19-440A-1 10 µg
EUR 383

"S" SARS-CoV-2 Spike Omicron mRNA (20 µg, Transfection-ready)
CVD19-445A-1 20 µg
EUR 553

HR based Kit in MOUSE 3T3 Cells Requiring I-Sce1 Transfection (100ug DNA)
DR3000-HR3T3-Sce2 100ug DNA
EUR 3486

HR based Kit in MOUSE 3T3 Cells Requiring I-Sce1 Transfection (250ug DNA)
DR3000-HR3T3-Sce3 250ug DNA
EUR 4110

HR based Kit in HeLa Requiring I-Sce1 Transfection (50ug DNA)
DR3000-HRHeLa-Sce 50ug DNA
EUR 3174

"S" SARS-CoV-2 Spike Glycoprotein mRNA (10 µg, Transfection-ready)
CVD19-430A-1 10 µg
EUR 360

"S" SARS-CoV-2 Spike Glycoprotein mRNA (20 µg, Transfection-ready)
CVD19-435A-1 20 µg
EUR 520

HR based Kit in Mouse Neuronal Cells Requiring I-Sce1 Transfection (100ug DNA)
DR3000-HRCN1.4-Sce2 100ug DNA
EUR 3735.6

HR based Kit in Mouse Neuronal Cells Requiring I-Sce1 Transfection (250ug DNA)
DR3000-HRCN1.4-Sce3 250ug DNA
EUR 4359.6

Transfection Collection™ : AP1 Transient Pack (JNK Signaling Pathway)
79266 500 rxns.
EUR 715
Description: The AP1 Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the JNK signaling pathway and the transcriptional activity of AP1 in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a JNK pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the AP1 Transient Pack is the AP1 luciferase reporter vector. This reporter contains the firefly luciferase gene under the control of multimerized AP1 responsive elements located upstream of a minimal promoter. The AP1 reporter is premixed with a constitutively-expressing Renilla luciferase vector that serves as an internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively- expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 and HeLa cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. _x000D_*Note: the kit may be used with other cell lines than HEK293 or HeLa, but an alternate cell culture medium may be required for optimal cell growth

Transfection Collection™ : Myc Transient Pack (Myc Signaling Pathway)
79284 500 rxns.
EUR 750
Description: The Myc Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the Myc signaling pathway in cultured HCT116 cells. The Myc Transient Pack contains transfection-ready vectors containing firefly luciferase as a Myc pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HCT116 cells._x000D_.The key to the Myc Transient Pack is the expression vectors for c-Myc and Myc luciferase reporter vector. Inside the cells, c-Myc will bind to Max, translocate to the nucleus, and induce expression of the Myc luciferase reporter vector. This reporter contains the firefly luciferase gene under the control of multimerized Myc responsive elements located upstream of a minimal promoter. The Myc reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. This negative control is critical for determining pathway-specific effects and background luciferase activity. An "empty" expression vector without the c-Myc gene is also provided as an additional negative control._x000D_Additionally, the pack includes cell culture medium that has been optimized for use with HCT116 cells*. HCT116 is a human colon cancer cell line with a mutated β -catenin which leads to the accumulation of β-catenin and constitutive activation of the Myc signaling pathway. This medium includes 10% fetal bovine serum, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HCT116, but an alternate cell culture medium may be required for optimal cell growth. For HEK293 cells, we recommend using BPS Medium 1 (BPS Bioscience, #79259).

NHEJ Based in Human Neuroblastoma Line requires I-Sce1 transfection (100ug DNA)
DR5000-HNSH-Sce2 100ug DNA
EUR 3610.8

NHEJ Based in Human Neuroblastoma Line requires I-Sce1 transfection (250ug DNA)
DR5000-HNSH-Sce3 250ug DNA
EUR 4234.8

NHEJ Based in Mouse Neuronal CN1.4 cells requires I-Sce1 transfection (100ug DNA)
DR5000-HNCN1.4-Sce2 100ug DNA
EUR 3486

NHEJ Based in Mouse Neuronal CN1.4 cells requires I-Sce1 transfection (250ug DNA)
DR5000-HNCN1.4-Sce3 250ug DNA
EUR 4110

HR in human Neuroblastoma Cells req. I-Sce1 Transfection (50ug DNA)
DR3000-HRSH-Sce 50ug DNA
EUR 3423.6

NHEJ Based Mouse 3T3 cells Requires I-Sce1 Transfection (50ug DNA)
DR5000-HN3T3-Sce 50ug DNA
EUR 3174

HR based Kit in MOUSE 3T3 Cells Requiring I-Sce1 Transfection (50ug DNA)
DR3000-HR3T3-Sce 50ug DNA
EUR 3174

Transfection Collection™ : SRE Transient Pack (MAPK/ERK Signaling Pathway)
79271 500 rxns.
EUR 750
Description: The SRE Reporter Kit Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the ERK signaling pathway and the transcriptional activity of SRE in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a MAPK/ERK pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the SRE Transient Pack is the SRE luciferase reporter vector, which is an ERK pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized SRE responsive elements located upstream of a minimal promoter. The SRE reporter is premixed with a constitutively-expressing Renilla luciferase vector that serves as an internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively- expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_* Other mammalian cell lines may also be used, but an alternate cell culture medium may be required for optimal cell growth.

Transfection-ready Cas9 SmartNickase mRNA (Eukaryotic Nickase mutant version)
CAS504A-1 20 ug
EUR 387

ExoFectin Plasmid DNA-into-Exosome Kit (DNA transfection kit for exosome)
P402 - Ask for price

Transfection Collection™ : SBE Transient Pack (TGFβ/SMAD Signaling Pathway)
79272 500 rxns.
EUR 750
Description: The SBE Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of TGFβ/SMAD signaling pathway in the cultured cells. The kit contains transfection-ready vectors containing firefly luciferase as a TGFβ/SMAD pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the SBE Transient Pack is the SBE luciferase reporter vector, which is a TGFβ pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized SBE responsive element located upstream of a minimal promoter. The SBE reporter is premixed with constitutively expressing Renilla luciferase vector that serves as internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. _x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth.

Transfection Collection™ : NF-κB Transient Pack ( NF-κB Signaling Pathway)
79268 500 rxns.
EUR 780
Description: The NF-κB Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the NF-κB signaling pathway in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a NF-κB pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the NF-κB Transient Pack is the NF-κB luciferase reporter vector. This reporter contains a firefly luciferase gene under the control of multimerized NF-κB responsive element located upstream of a minimal promoter. The NF-κB reporter is premixed with constitutively-expressing Renilla luciferase vector, which serves as an internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth.

Transfection Collection™ : Notch1/CSL Transient Pack (Notch Signaling Pathway)
79285 500 rxns.
EUR 715
Description: The Notch1/CSL Transient Packis designed to provide the tools necessary for transiently transfecting and monitoring the activity of the Notch signaling pathway in HEK293 cultured cells. The kit contains transfection-ready vectors containing firefly luciferase as a Notch pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the Notch1/CSL Transient Pack is the expression vector for NOTCH1 that has a deletion of the entire extracellular domain (Notch1DE). Inside the cells, the NOTCH1 DE can be cleaved by γ-secretase and active NOTCH1 NICD is released into the nucleus. The kit also contains CSL (CBF1/RBP-Jk) luciferase reporter vector, which is a Notch pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized CSL responsive elements upstream of a minimal promoter. The CSL (CBF1/RBP-Jk) reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. _x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth,

HR based Kit in Mouse Neuronal Cells Requiring I-Sce1 Transfection (50ug DNA)
DR3000-HRCN1.4-Sce 50ug DNA
EUR 3423.6

The Transfection Collection™ : ISRE Transient Pack JAK/STAT Signaling Pathway
79264 500 rxns.
EUR 715
Description: The ISRE Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of Type I interferon-induced JAK/STAT signaling pathway in cultured HEK293 cells. The pack contains transfection-ready vectors containing firefly luciferase as a JAK/STAT pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the ISRE Transient Pack is the ISRE luciferase reporter vector, which is a JAK/STAT pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized ISRE responsive element located upstream of a minimal promoter. The ISRE reporter is premixed with constitutively-expressing Renilla luciferase vector, which serves as an internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth.

PEI MAX® - Transfection Grade Linear Polyethylenimine Hydrochloride (MW 40,000)
24765-1 1g
EUR 1126
Description: 49553-93-7

PEI MAX® - Transfection Grade Linear Polyethylenimine Hydrochloride (MW 40,000)
24765-100 100mg
EUR 219
Description: 49553-93-7

Transfection Collection™ : CRE/CREB Transient Pack (cAMP/PKA Cell Signaling Pathway)
79267 500 rxns.
EUR 715
Description: The CRE/CREB Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the cAMP/PKA signaling pathway in cultured cells. The kit contains transfection-ready vectors containing firefly luciferase as a cAMP/PKA pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the CRE/CREB Transient Pack is the CRE/CREB luciferase reporter vector, which is a cAMP/PKA Cell Signaling Pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized cAMP response elements (CRE) located upstream of a minimal promoter. Elevation of the intracellular cAMP level activates cAMP response element binding protein (CREB) to bind CRE and induces the expression of luciferase._x000D_The CRE reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with the constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_* Other mammalian cell lines may also be used, but an alternate cell culture medium may be required for optimal cell growth.

NHEJ Based in Human Neuroblastoma Line requires I-Sce1 transfection (50ug DNA)
DR5000-HNSH-Sce 50ug DNA
EUR 3298.8

NHEJ Based in Mouse Neuronal CN1.4 cells requires I-Sce1 transfection (50ug DNA)
DR5000-HNCN1.4-Sce 50ug DNA
EUR 3174

The Transfection Collection™ : GAL4 Transient Pack Glucocorticoid Receptor Pathway
79265 500 rxns.
EUR 715
Description: The GAL4 Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the glucocorticoid signaling pathway in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a glucocorticoid pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the GAL4 Transient Pack is the expression vector for the glucocorticoid receptor ligand binding domain that is fused to the DNA binding domain (DBD) of GAL4 (GAL4 DBD-GR). This fusion construct activates firefly luciferase expression under the control of a multimerized GAL4 upstream activation sequence (UAS). This allows for specific detection of glucocorticoid-induced activation of the glucocorticoid receptor without the need for individual transcriptional targets and with low cross-reactivity for other nuclear receptor pathways. The GAL4/UAS reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway-specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth.

Transfection Collection™ : TCF/LEF Transient Pack Wnt / β-catenin Signaling Pathway
79273 500 rxns.
EUR 750
Description: The TCF/LEF Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of Wnt / Beta-catenin signaling pathway in the cultured HEK293 cells. The TCF/LEF Transient Pack contains transfection-ready vectors containing firefly luciferase as a Wnt pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the TCF/LEF Transient Pack is the TCF/LEF luciferase reporter vector, which is a Wnt pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized TCF/LEF responsive element located upstream of a minimal promoter. The TCF/LEF reporter is premixed with constitutively expressing Renilla luciferase vector that serves as internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth.

Transfection-ready hspCas9-T2A-GFP SmartNuclease mRNA (wildtype Cas9 with GFP marker)
CAS530G-1 10 ug
EUR 342

Transfection-ready hspCas9-T2A-RFP SmartNuclease mRNA (wildtype Cas9 with RFP marker)
CAS531R-1 10 ug
EUR 342

EZ-DNA Reagents
BS8202 100preps
EUR 105.67

EZ-DNA Reagents
SK8201 100preps
EUR 112.2

Transfection-ready hspCas9-nickase-T2A-GFP SmartNickase mRNA (Cas9 Nickase mutant with GFP marker)
CAS534G-1 10 ug
EUR 342

Transfection-ready hspCas9-nickase-T2A-RFP SmartNickase mRNA (Cas9 Nickase mutant with RFP marker)
CAS535R-1 10 ug
EUR 342

Ecl Detection Reagents
RPN2105 EACH
EUR 724.8

Ecl Detection Reagents
RPN3004 EACH
EUR 582

REAGENTS HIGH RANGE AMMONIA
HI73325 PK25
EUR 49.2

High Range Ammonia Reagents
HI9373301 EACH
EUR 116.4

CryoProtX Mix Reagents 1.5 mL
M-MDSR-61 1.5 ml ml
EUR 37
Description: CryoProtX Mix Reagents 1.5 mL

Alkphos Direct Labelling Reagents
RPN3680 EACH
EUR 813.6

Set of 10 Biolipidure Reagents
Biolipidure-set 10mLx10
EUR 1820.4
Description: Set of 10 Biolipidure Reagents, whose applications include Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

CryoProtX Mix Eco Reagents 1.5 mL
M-MDSR-61-ECO 1.5 ml ml
EUR 37
Description: CryoProtX Mix Eco Reagents 1.5 mL

Free Chlorine Reagents 0-5mg/L
WAT1028 EACH
EUR 44.4

Bradford(Coomassie) Protein Assay Plus Reagents
P7201-050 450ml
EUR 225.6

Uncoated Ancillary Reagent Kits
RK04587 20Plates
EUR 280

Amersham ECL Western Blotting Detection Reagents for 4000cm2 membrane
RPN2106 EACH
EUR 374.4

Amersham ECL Western Blotting Detection Reagents for 1000cm2 membrane
RPN2109 EACH
EUR 188.4

Amersham ECL Western Blotting Detection Reagents for 6000cm2 membrane
RPN2134 1KIT
EUR 572.4

Amersham ECL Western Blotting Detection Reagents for 2000cm2 membrane
RPN2209 EACH
EUR 291.6

ImmunoGold labeling reagents for (TEM) - Unconjugated gold colloid (GC), 5nm
22716-100 100ml
EUR 932
Description: 7732-18-5

ImmunoGold labeling reagents for (TEM) - Unconjugated gold colloid (GC), 10nm
22717-100 100ml
EUR 919
Description: 7732-18-5

ImmunoGold labeling reagents for (TEM) - Unconjugated gold colloid (GC), 15nm
22718-100 100ml
EUR 920
Description: 7732-18-5

ImmunoGold labeling reagents for (SEM) - Unconjugated gold colloid (GC) 20nm
22719-100 100ml
EUR 932

Gentodenz
19-DENZ-500 500 g
EUR 416

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)
CAS510A-KIT 1 Kit
EUR 724

Exo-Flow 2.0 Basic Kit without antibody (Streptavidin beads + reagents) - for Serum or Plasma
EXOFLOW2-BASICA-SP 30 rxn
EUR 1201.2

TruStrip WBV Tubulin protein quantitation & Western Blot Validation Kit (contains actin strips, primary antibodies and Western reagents 100 strips or 10 miniblot)
WBTUB100-K 1 Kit
EUR 634.8

Exo-Flow 2.0 Basic Kit without antibody (Streptavidin beads + reagents) - for Tissue Culture Media
EXOFLOW2-BASICA-TC 30 rxn
EUR 1201.2

cAMP Biotrak(TM) EIA; (Non-Acetylation Protocol; Lysis Reagents not Included); Cytiva; RPN2251
GERPN2251 EACH
EUR 685.2

TruStrip WBV Human Beta Actin Protein Quantitation & Western Blot Validation Kit (contains actin strips, primary antibodies and Western reagents 100 strips or 10 miniblot)
WBACT100-K 1 Kit
EUR 634.8

TransDux virus transduction reagent (200x)
LV850A-1 80 transductions
EUR 257

Monkeypox Virus Real Time PCR Kit
ZD-0076-01 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids. The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long. The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of the Monkeypox Virus DNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channel 530nm with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the 560nm fluorescence of the internal control (IC). An external positive control defined as 1×10^7 copies/ml is supplied which allow the determination of the gene load.

Monkeypox Virus Real Time PCR Kit
ZD-0076-02 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids.The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long.The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of theMonkeypox VirusDNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channelFAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load.

TruStrip WBV Mouse Glyceraldehyde phosphate dehydrogenase (GAPDH/G3PDH) protein quantitation & Western Blot Validation Kit (contains actin strips, primary antibodies and Western reagents 100 strips or 10 miniblot)
WBGPD100-K 1 Kit
EUR 634.8

TruStrip WBV Human Glyceraldehyde phosphate dehydrogenase (GAPDH/G3PDH) protein quantitation & Western Blot Validation Kit (contains actin strips, primary antibodies and Western reagents 100 strips or 10 miniblot)
WBGPD200-K 1 Kit
EUR 634.8

Sample Transport Reagent
SSFIN-0007 20mL
EUR 32

ASTM Type I and ACS Reagent Grade Water
H2OASTM1 1L
EUR 36

EP Reagent 0.1M Ammonium and Cerium Nitrate
3000100 1L
EUR 354

EP Reagent Potassium Iodide and Starch Sol.
1070501 125ML
EUR 63.6

EP Reagent 0.01M Ammonium and Cerium Nitrate
3000200 1L
EUR 121.2

EP Reagent 0.1M Ammonium and Cerium Sulphate
R3000300 1L
EUR 474

Antibody Dilution and Stabilizer Reagent 10X
22050007-1 25 mL
EUR 32.46

Antibody Dilution and Stabilizer Reagent 10X
22050007-2 100 mL
EUR 69.62

Albumin Depletion Reagent for Plasma and Serum (20 ml)
P5W13 - Ask for price

Ceratase, Gentle Non-animal Source Dissociation Reagent
CA096-010 100ml Ask for price

QuantiChrom Hemoglobin Assay Kit
65-DIHB-250 250 tests
EUR 473

Malachite Green Phosphate Assay kit
65-POMG-25H 2500 tests
EUR 333

Porcine Parvovirus Antibody Elisa Test Kit
767-LSY-30009 192 Wells/kit
EUR 382

Genorise® GSI RNA Reagent, 200 ml
GR102015 200
EUR 342

Genorise® GSI RNA Reagent, 100 ml
GR102034 100
EUR 193

50ml TC Tubes, Conical, 440 units/box
04-5540150 440 units/box
EUR 85.2

GMP IL4, 50µg
04-GMP-HU-IL4-50UG 50µg
EUR 579.6
Description: Recombinant Human IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton. The rHuIL-4 is purified by proprietary chromatographic techniques.
The potential utility of this artificial strategy is demonstrated by a late-stage modification of diethylstilbestrol. Mechanistic research revealed the a number of essential roles performed by the Ruppert-Prakash reagent.

Dalton

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