Fluorine-18 labelled Ruppert-Prakash reagent ([ 18 F]Me 3 SiCF 3) for the synthesis of 18 F-trifluoromethylated compounds

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This text describes the primary synthesis and utility of fluorine-18 labelled Ruppert-Prakash reagent [18F]Me3SiCF3. [18F]Me3SiCF3 was synthesized from [18F]fluoroform with radiochemical yields of 85-95% and radiochemical purities of >95% inside 20 minutes. 18F-trifluoromethylated compounds had been efficiently ready by response of [18F]Me3SiCF3 with benzaldehydes, acetophenones and benzophenones.
Intact part II steroid metabolites have poor product ion mass spectra beneath collision induced dissociation (CID) situations. Due to this fact, we current herein the liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/(MS)) habits of intact part II metabolites of oxosteroids after derivatization.
Based mostly on the truth that Girard’s reagent T (GRT), as derivatization reagent, was each handy and environment friendly by way of the enhancement within the ionization effectivity and the manufacturing of diagnostic product ions associated to the steroid moiety, the latter was ideally chosen between methoxamine and hydroxylamine upon the mannequin compounds of androsterone glucuronide and androsterone sulfate.
16 totally different glucuronide and 29 sulfate conjugated metabolites of anabolic androgenic steroids (AAS), out there both as pure reference supplies, or synthesized/extracted from administration research, had been derivatized with GRT and their product ion spectra are offered. Product ion spectra embrace in all instances excessive variety of product ions that in some instances are attribute for sure constructions of the steroid spine.
Extra particularly, preliminary outcomes have proven main variations in fragmentation sample for 17α/17β-isomers of the sulfate-conjugates, however restricted differentiation for 17α/17β-isomers of glucuronide-conjugates and for 3α/3β- and 5α/5β-stereoisomers of each sulfate and glucuronide conjugates. Additional to the suggestion of the present work, utility on mesterolone administration research confirmed -according to the WADA TD2015IDCR- the presence of seven intact part II metabolites, one glucuronide and 6 sulfates with use of LC-ESI-MS/(MS).

Dedication of nitrofuran metabolites in honey utilizing a brand new derivatization reagent, magnetic solid-phase extraction and LC-MS/MS

On this research, 5-nitro-2-furaldehyde (5-NFA) was proposed as a brand new derivatizing agent for nitrofuran metabolites. It reacts with nitrofuran metabolites producing the guardian nitrofurans (furazolidone, furaltadone, nitrofurantoin, and nitrofurazone). Magnetic hypercrosslinked polystyrene (HCP/Fe3O4) was first used for magnetic stable part extraction (MSPE) clean-up earlier than the dedication of nitrofuran metabolite derivatives in honey by way of LC-MS/MS.
Primary parameters affecting the derivatization and MSPE effectivity had been investigated intimately and the optimum situations had been discovered. The tactic was validated utilizing honey spiked with the 4 metabolites at 1, 2 and 200 μg kg-1. Recoveries of >85% had been achieved for the all analytes. The matrix calibration curve was fitted with the correlation coefficient (R2) > 0.99 within the vary of 1-200 μg kg-1.
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Precision values expressed as relative commonplace deviation (RSD) had been <12% and <15% for intra-day and inter-day precision, respectively. The boundaries of detection (LODs) for the nitrofuran metabolites had been of 0.1-0.Three μg kg-1 and the bounds of quantitation (LOQs) had been of 0.3-1.Zero μg kg-1. The proposed LC-MS/MS technique was utilized to the evaluation of actual honey samples.

Peptide Bond Formation of Amino Acids by Transient Masking with Silylating Reagents

A one-pot peptide bond-forming response has been developed utilizing unprotected amino acids and peptides. Two totally different silylating reagents, HSi[OCH(CF3)2]3 and MTBSTFA, are instrumental for the profitable implementation of this strategy, getting used for the activation and transient masking of unprotected amino acids and peptides at C-termini and N-termini, respectively.
Moreover, CsF and imidazole are used as catalysts, activating HSi[OCH(CF3)2]3 and in addition accelerating chemoselective silylation. This technique is flexible because it tolerates facet chains that bear a variety of useful teams, whereas offering as much as >99% yields of corresponding peptides with none racemization or polymerization.

Hydrothermal Crystallization of Bismuth Oxychlorides (BiOCl) Utilizing Completely different Form Management Reagents

Bismuth oxychloride photocatalysts had been obtained utilizing solvothermal synthesis and totally different components (CTAB-cetyltrimethylammonium bromide, CTAC-cetyltrimethylammonium chloride, PVP-polyvinylpyrrolidone, SDS-sodium dodecylsulphate, U-urea and TU-thiourea). The impact of the beforehand talked about compounds was analyzed making use of structural (main crystallite measurement, crystal part composition, and so forth.), morphological (particle geometry), optical (band hole vitality) parameters, floor associated properties (floor atoms’ oxidation states), and the resulted photocatalytic exercise.
A powerful dependency was discovered between the floor pressure of the synthesis options and the general morpho-structural parameters. The primary discovering was that the traits of the semiconductors could be tuned by modifying the floor pressure of the synthesis combination. It was noticed after the photocatalytic degradation, that the white semiconductor turned to gray. Moreover, we tried to clarify the grey shade of BiOCl catalysts after the photocatalytic decompositions by Raman and XPS research.

Difluorocarbene-Mediated Cascade Cyclization: The Multifunctional Function of Ruppert-Prakash Reagent

A difluorocarbene-mediated cascade cyclization response for fast entry to gem-difluorinated 3-coumaranone derivatives was developed. The difluorocarbene acts as a bipolar CF2 constructing block, which permits a homologation cyclization course of by way of sequentially reacting with the phenolate and the ester group on the identical substrate.

Basic Cytotoxicity Test Kits

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Set of 10 Biolipidure Reagents

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Convoy? Transfection Reagent

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MITOCHONDRIAL MEMBRANE POTENTIAL KIT Kits (OKSA11298)

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MITOCHONDRIAL MEMBRANE POTENTIAL KIT Kits (OKSA11300)

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I7200-101 1ml
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Transfection-ready Cas9 SmartNuclease AAVS1 gRNA

CAS520A-1 10 ug
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EXFT200A-1 20 reactions
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Multiplex ELISA Kits for IL1α, IL1β, IL6, IL8, GMCSF, INF-γ, MCAF and TNFα, human

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50ml TC Tubes, Conical, 440 units/box

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GMP IL4, 50µg

04-GMP-HU-IL4-50UG 50µg
EUR 483
Description: Recombinant Human IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton. The rHuIL-4 is purified by proprietary chromatographic techniques.

rHu IL 2 , 3MIU

04-RHIL2-02F01 1 vial
EUR 249
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.

rHu IL 2 , 3MIU , Lot 200908F02

04-RHIL2-08F02 1 vial
EUR 249
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.

PRE-GMP rHu GM-CSF, Molgramostim-Leukoma

04-RHUGM-CSF-7A10 300 µg
EUR 385
Description: Recombinant human GM-CSF produced in E.coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids, two pairs of disulfide bonds and having a molecular mass of approximately 14.5kD.

Transfection-ready Cas9 SmartNuclease mRNA (Eukaryotic version)

CAS500A-1 20 ug
EUR 445
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Mast/stem Cell Growth Factor Receptor Kit (Kits) Antibody

20-abx116620
  • EUR 732.00
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Rabbit ANTI 14-3-3 ISOFORM PANEL Kits (OKSA11258)

OKSA11258 1 u
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T-Pro Nonliposomal Transfection Reagent I (NTR I)

JT97-N001M 1.0ml/vial
EUR 187

T-Pro Nonliposomal Transfection Reagent II (NTR II)

JT97-N002M 1.0ml/vial
EUR 204

T-Pro Nonliposomal Transfection Reagent III (NTR III)

JT97-N006M 1.0ml/vial
EUR 222

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)

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NHEJ Based HeLa Requires I-Sce1 Transfection (50ug DNA)

DR5001-HNHela-Sce 50ug DNA
EUR 2645

NHEJ Based HeLa Requires I-Sce1 Transfection (100ug DNA)

DR5001-HNHela-Sce2 100ug DNA
EUR 2905

NHEJ Based HeLa Requires I-Sce1 Transfection (250ug DNA)

DR5001-HNHela-Sce3 250ug DNA
EUR 3425

Transfection-ready Cas9 SmartNickase mRNA (Eukaryotic Nickase mutant version)

CAS504A-1 20 ug
EUR 445
  • Category: Cas9

TruStrip WBV Tubulin protein quantitation & Western Blot Validation Kit (contains actin strips, primary antibodies and Western reagents 100 strips or 10 miniblot)

WBTUB100-K 1 Kit
EUR 529

HR based Kit in HeLa Requiring CRISPR Transfection (50ug DNA)

DR3000-HRHeLa-CPR 50ug DNA
EUR 2749

HR based Kit in HeLa Requiring CRISPR Transfection (100ug DNA)

DR3000-HRHeLa-CPR2 100ug DNA
EUR 3009

HR based Kit in HeLa Requiring CRISPR Transfection (250ug DNA)

DR3000-HRHeLa-CPR3 250ug DNA
EUR 3529

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EUR 1001
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TruStrip WBV Human Beta Actin Protein Quantitation & Western Blot Validation Kit (contains actin strips, primary antibodies and Western reagents 100 strips or 10 miniblot)

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EUR 529

HR based Kit in HeLa Requiring I-Sce1 Transfection (50ug DNA)

DR3000-HRHeLa-Sce 50ug DNA
EUR 2645

HR based Kit in HeLa Requiring I-Sce1 Transfection (100ug DNA)

DR3000-HRHeLa-Sce2 100ug DNA
EUR 2905

HR based Kit in HeLa Requiring I-Sce1 Transfection (250ug DNA)

DR3000-HRHeLa-Sce3 250ug DNA
EUR 3425

HR in human Neuroblastoma Cells req. I-Sce1 Transfection (50ug DNA)

DR3000-HRSH-Sce 50ug DNA
EUR 2853

HR in human Neuroblastoma Cells req. I-Sce1 Transfection (100ug DNA)

DR3000-HRSH-Sce2 100ug DNA
EUR 3113

HR in human Neuroblastoma Cells req. I-Sce1 Transfection (250ug DNA)

DR3000-HRSH-Sce3 250ug DNA
EUR 3633

NHEJ Based Mouse 3T3 cells Requires I-Sce1 Transfection (50ug DNA)

DR5000-HN3T3-Sce 50ug DNA
EUR 2645

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DR5000-HN3T3-Sce2 100ug DNA
EUR 2905

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EUR 3425

ExoFectin Plasmid DNA-into-Exosome Kit (DNA transfection kit for exosome)

P402 NULL
EUR 0

NHEJ Based in Human Neuroblastoma Line requires I-Sce1 transfection (50ug DNA)

DR5000-HNSH-Sce 50ug DNA
EUR 2749

NHEJ Based in Human Neuroblastoma Line requires I-Sce1 transfection (100ug DNA)

DR5000-HNSH-Sce2 100ug DNA
EUR 3009

NHEJ Based in Human Neuroblastoma Line requires I-Sce1 transfection (250ug DNA)

DR5000-HNSH-Sce3 250ug DNA
EUR 3529

Transfection-ready hspCas9-T2A-GFP SmartNuclease mRNA (wildtype Cas9 with GFP marker)

CAS530G-1 10 ug
EUR 400
  • Category: Cas9

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CAS531R-1 10 ug
EUR 400
  • Category: Cas9

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EUR 529

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EUR 834

HR based Kit in MOUSE 3T3 Cells Requiring I-Sce1 Transfection (50ug DNA)

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EUR 2645

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EUR 2905

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EUR 2645

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DR5000-HNCN1.4-Sce2 100ug DNA
EUR 2905

NHEJ Based in Mouse Neuronal CN1.4 cells requires I-Sce1 transfection (250ug DNA)

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EUR 3425

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910-805-THM 1 Kit
EUR 712

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910-815-TMM 1 Kit
EUR 834

Transfection-ready hspCas9-nickase-T2A-GFP SmartNickase mRNA (Cas9 Nickase mutant with GFP marker)

CAS534G-1 10 ug
EUR 400
  • Category: Cas9

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CAS535R-1 10 ug
EUR 400
  • Category: Cas9

Tissue and Cell Extraction and Purification

EP10011 100 Tests
EUR 636

p5 Ligand for Dnak and and DnaJ

5-01680 4 x 5mg Ask for price

Linsmaier and Skoog, With macro- and micronutrients

CP021-010 10X1L
EUR 101

Linsmaier and Skoog, With macro- and micronutrients

CP021-500 50L
EUR 126

Murashige and Skoog, With Vitamins and Glycine

CP031-010 10X1L
EUR 99

Murashige and Skoog, With Vitamins and Glycine

CP031-500 50L
EUR 126

p5 Ligand for Dnak and and DnaJ Peptide

20-abx266082
  • EUR 411.00
  • EUR 662.00
  • EUR 314.00
  • 10 mg
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  • 5 mg
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Murashige and Skoog

CP023-010 10X1L
EUR 99

Murashige and Skoog

CP023-500 50L
EUR 126

Nitsch and Nitsch

CP036-010 10X1L
EUR 101

Nitsch and Nitsch

CP036-500 50L
EUR 126

Shenk and Hildebrandt

CP038-010 10X1L
EUR 101

Shenk and Hildebrandt

CP038-500 50L
EUR 126

Recombinant Human AND

P0015 100ug
EUR 522.36
  • Formulation: pH7.4, Lyophilized from a 0.2um filtered solution in PBS with 5% trehalose
  • Reconstitution: Sterile distilled water
  • Purity: Greater than 95% by SDS-PAGE gel analyses
  • Uniprot ID: Q15848
Description: Recombinant Human protein for AND

Mouse Ariway PrimaCell3: Normal Bronchial and and Tracheal Fibroblasts

2-82003 1 Kit Ask for price

Rat Ariway PrimaCell3: Normal Bronchial and and Tracheal Fibroblasts

2-82503 1 Kit Ask for price

Human Airway PrimaCell4: Normal Bronchial and and Tracheal Fibroblasts

2-96004 1 Kit Ask for price

Murashige and Skoog, 1/2 Micronutrients and 1/2 Iron

CP024-010 10X1L
EUR 99

Murashige and Skoog, 1/2 Micronutrients and 1/2 Iron

CP024-500 50L
EUR 126

Murashige and Skoog, With Vitamins, Glycine, and 30g/L Sucrose

CP032-010 10X1L
EUR 101

Murashige and Skoog, With Vitamins, Glycine, and 30g/L Sucrose

CP032-500 50L
EUR 126

Phosphoserine and Phosphothreonine antibody

70R-10686 1 ml
EUR 373
Description: Rabbit polyclonal Phosphoserine and Phosphothreonine antibody

5-(and-6)-carboxyfluorescein

51013 100MG
EUR 100
Description: Minimum order quantity: 1 unit of 100MG

Protease, Phosphatase and PMSF

abx090639-1ml 1 ml
EUR 230
  • Shipped within 5-10 working days.

cool-pack and platfom

EHS1100-COOL ea
EUR 90

cool-pack and platfom

EHS1200-COOL ea
EUR 107

cool-pack and platfom

EHS1300-COOL ea
EUR 124

cool-pack and platfom

EHS1400-COOL ea
EUR 124

cool-pack and platfom

EHS1500-COOL ea
EUR 149

OX1R and OX2R Antibody

abx431748-200ul 200 ul
EUR 384
  • Shipped within 1-3 working days.

BAF53A and BAF53B Antibody

abx432390-200ul 200 ul
EUR 384
  • Shipped within 1-3 working days.

CBX6 and CBX8 Antibody

abx432458-200ul 200 ul
EUR 286
  • Shipped within 1-3 working days.

CCL3 and CCL3L1 Antibody

abx432463-200ul 200 ul
EUR 286
  • Shipped within 1-3 working days.

RFPL2 and RFPL3 Antibody

abx433225-200ul 200 ul
EUR 286
  • Shipped within 1-3 working days.

SUR1 and SUR2B Antibody

abx440192-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SUR1 and SUR2B Antibody

abx440473-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SUR1 and SUR2B Antibody

abx440754-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SUR1 and SUR2B Antibody

abx441035-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SUR1 and SUR2B Antibody

abx441316-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SUR1 and SUR2B Antibody

abx441597-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SUR1 and SUR2B Antibody

abx441878-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SUR1 and SUR2B Antibody

abx442159-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SUR1 and SUR2B Antibody

abx442440-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SUR1 and SUR2B Antibody

abx442721-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SUR1 and SUR2B Antibody

abx443001-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SUR1 and SUR2B Antibody

abx443281-100ug 100 ug
EUR 565
  • Shipped within 5-12 working days.

SUR1 and SUR2B Antibody

abx443562-100ug 100 ug
EUR 565
  • Shipped within 5-12 working days.

SUR1 and SUR2B Antibody

abx443843-100ug 100 ug
EUR 592
  • Shipped within 5-12 working days.

SUR1 and SUR2B Antibody

abx444124-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SUR1 and SUR2B Antibody

abx444405-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SUR1 and SUR2B Antibody

abx444686-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SUR1 and SUR2B Antibody

abx445043-100ug 100 ug
EUR 523
  • Shipped within 5-12 working days.

USB MOUSE AND KEYBOARD

GD-USBMK 1/pk
EUR 201
Description: Lab Equipment; Axygen Branded EQ

BRCA2 and CDKH1A Interacting

PR27247 2 ug
EUR 191

Endomorphin 1 and 2

RA21002 50 ug
EUR 265

Blunting and Ligation Kit

BS243 10Preps
EUR 102.2
  • Product category: Molecular Biology Kits/Blunting/Ligation

RNase and DNAase Away

DB0339 200ml
EUR 72.62
  • Product category: Biochemicals/Biology Reagents/DNase/RNase Related

Linsmaier and Skoog, pH buffered to 5.7 +/-0.1 With macro- and micronutrients and 30 g/L sucrose

CP019-010 10X1L
EUR 101

Linsmaier and Skoog, pH buffered to 5.7 +/-0.1 With macro- and micronutrients and 30 g/L sucrose

CP019-500 50L
EUR 126

SIDEARM FITTING, DELIVERY AND VENT, 1/8 AND 1/4 INLET

4519-104 1/pk
EUR 458
Description: Bioprocess Vessels; Spinner Accessories

Neural Dissociation System 3 (Neurons, DRG and SCG), Mouse and Rat

4-20353 ea Ask for price

Skin Dissociation System 1 (Dermal fibroblast and keratinocytes), Mouse and Rat

4-20431 ea Ask for price

Rat Ariway PrimaCell3: Normal Bronchial and and Tracheal Fibroblasts Growth Medium

9-25003 5 x 100 ml Ask for price

Mouse Ariway PrimaCell3: Normal Bronchial and and Tracheal Fibroblasts Growth Medium

9-32003 5 x 100 ml Ask for price

Human Airway PrimaCell4: Normal Bronchial and and Tracheal Fibroblasts Growth Medium

9-46004 5 x 100 ml Ask for price

DMEM, With high-glucose and L-glutamine. and 25 mM HEPES,

CMP04-001 10x1L
EUR 99

DMEM, With high-glucose and L-glutamine. and 25 mM HEPES,

CMP04-010 10L
EUR 84

DMEM, With high-glucose and L-glutamine. and 25 mM HEPES,

CMP04-050 50L
EUR 142

Murashige and Skoog, With 1/2 X (NH4NO3, KNO3, and CaCL2)

CP026-010 10X1L
EUR 99

Murashige and Skoog, With 1/2 X (NH4NO3, KNO3, and CaCL2)

CP026-500 50L
EUR 126

ExoQuick Exosome Isolation and RNA Purification kit (for Serum and Plasma)

EQ806A-1 20 preps
EUR 554

Streptococcus group A and pyogenic group C and G PCR kit

PCR-H705-48D 50T
EUR 425.8
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Streptococcus group A and pyogenic group C and G

Streptococcus group A and pyogenic group C and G PCR kit

PCR-H705-96D 100T
EUR 521.5
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Streptococcus group A and pyogenic group C and G

XCF COMPLETE Exosome and cfDNA Isolation Kit (for Serum and Plasma)

XCF100A-1 20 rxn
EUR 647
  • Category: Exosome

Epithelial Dissociation System 9 (Mesothelial and surface epithelial, Ovaries), Mouse and Rat

4-20259 ea Ask for price

Neural Dissociation System 2 (Neurons, Neurons and glial,Retina), Mouse and Rat

4-20352 ea Ask for price

Human Airway Tissue Preparation Buffer 4: Normal Bronchial and and Tracheal Fibroblasts

9-80004 1 x 100 ml Ask for price

Mouse Ariway Tissue Preparation Buffer 3: Normal Bronchial and and Tracheal Fibroblasts

9-80117 1 x 100 ml Ask for price
The potential utility of this artificial strategy is demonstrated by a late-stage modification of diethylstilbestrol. Mechanistic research revealed the a number of essential roles performed by the Ruppert-Prakash reagent.

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