Product name: HRI, polyclonal antibody

Full Product Name: EIF2AK1 polyclonal antibody

Product synonym names

EIF2AK1, HRI, KIAA1369, eukaryotic translation initiation factor 2-alpha kinase 1, heme-controlled repressor, HCR, eukaryotic heme-regulated initiation factor eIF-2-alpha kinase, heme-regulated inhibitor, heme-sensitive initiation factor two

Product gene name: anti-HRI antibody

3D structure: ModBase 3D structure for Q9BQI3

Clonality: Polyclonal

Isotype: IgG

Host: Rabbit

Species reactivity: Human, Mouse, Monkey

Purity / Purification: Affinity purification

Shape / Format

PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH 7.4

Immunogen

Synthesized peptide derived from the C-terminal region of human HRI

Note

Centrifuge before opening to ensure complete recovery of the vial contents.

Preparation and storage

Store at -20 ° C. Avoid freeze/thaw cycles.

ISO certification

Manufactured in an ISO 9001: 2015 certified laboratory.

Other notes

Occasionally, small volumes of anti-HRI antibody vials may become trapped in the product vial seal during shipping and storage. If necessary, briefly centrifuge the vial in a benchtop centrifuge to dislodge any liquid on the lid of the container. Some products may need to be shipped with dry ice and an additional fee may apply for dry ice.

Related Product Information for anti-HRI antibody

Inhibits protein synthesis at the level of translation initiation, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock, and heat shock. It exerts its function through the phosphorylation of EIF2S1 in ‘Ser-48’ and ‘Ser-51’, thus avoiding their recycling. It binds to hemin forming a 1: 1 complex through nitrogen coordination of cysteine ​​thiolate and histidine. This binding occurs with moderate affinity, allowing you to detect the concentration of heme within the cell.

Thanks to this unique heme detection ability, plays a crucial role in stopping protein synthesis during acute heme deficiency conditions. In red blood cells (RBC), it controls the synthesis of hemoglobin, ensuring a coordinated regulation of the synthesis of its heme and globin residues. Therefore, it plays an essential protective role in the survival of red blood cells in iron deficiency anemia. Similarly, hepatocytes, involved in the heme-mediated translation control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. It can also contain ER stress during acute heme deficiency conditions.

Molecular weight

Calculated MW: 71 kDa
Observed MW: 71 kDa

Precautions

All MyBioSource products are for scientific laboratory research purposes and not for diagnostic, therapeutic, prophylactic, or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Product purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order when we reasonably believe that the intended use will be outside of our acceptable guidelines.

Disclaimer

While every effort has been made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be responsible for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.