Properties
Quality
Not suitable for clinical diagnostic use. It will not detect clinically relevant species such as M. pneumoniae and U. urealyticum
Quality level: 100
Packaging: 1 kit pack
Storage condition: dry at room temperature
Application(s)
PCR: adequate
detection: adequate
microbiology: adequate
Compatibility
Optimized for use with JumpStart ™ Taq DNA Polymerase, D9307.
Storage temperature: 2-8 ° C
General description
The LookOut® Mycoplasma PCR Detection Kit uses the polymerase chain reaction (PCR), which is established as the method of choice for the highest sensitivity in detecting Mycoplasma, Acholeplasma, and Ureaplasma contamination in cell cultures and other products. biological products derived from cell cultures.
The kit has been successfully tested for the ability to amplify Mycoplasma fermentans genomic DNA at concentrations of 20,000 to 20 copies. The reaction tubes included with the kit are precoated with appropriate dNTPs, primers, and loading dye. Total assay time is significantly reduced compared to general protocols that require individual loading of reaction tubes.
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Mycoplasma PCR Detection Kit Video Protocol
The LookOut Mycoplasma PCR Detection Kit has been used for the detection of mycoplasma infections.
Compatibility
The kit has been optimized for use with Product # D9307. Any DNA polymerase with 3′-5 ‘exonuclease activity is NOT recommended. These include Tli, Pfu, and Pwo. DNA polymerases that can be used include Taq, Tfl, and Tth.
